Patologia e clínica de equinos

O presente relatório é baseado no estágio curricular em clínica de equinos, realizado entre 1 de Setembro de 2015 e 29 de Fevereiro de 2016, com a orientação da Dra. Maria Teresa Félix da Cruz Falcão. É composto por três partes: casuística, monografia e apresentação de dois casos clínicos. A primeira parte descreve a casuística que se acompanhou durante o estágio, na área de patologia e clínica de equinos. A monografia aborda o tema de intoxicação por amitraz em equinos. A última parte consiste na descrição de dois casos clínicos de intoxicação por amitraz que foram acompanhados durante o estágio curricular; ABSTRACT: Externship report – Equine pathology and medicine The following report is based on my externship between the 1st of Setember of 2015 and the 29th of February of 2016, orientated by Dr. Maria Teresa Félix da Cruz Falcão in equine clinics. It is composed of three parts: casuistics, monography and the presentation of two clinical cases. The first part describes the casuistics that were encountered during the externship, in equine pathology and medicine. The monography discusses the theme of amitraz intoxication in horses. The last part is composed of a description and discussion of two clinical cases of amitraz intoxication, accompanied during the externship.

Establishment of antibody panels and histochemical techniques in routine tumor diagnosis in Veterinary Pathology

In Portugal, Veterinary Pathology is developing rapidly, and in recent years we assist to the emergence of private laboratories and the restructuring of universities,polytechnics and public laboratories.The Portuguese Society of Animal Pathology,through its actions and its associates has been keeping the discussion among its peers in order to standardizethe criteria of description,classification and evaluation of cases which are the subject of our daily work.One of the last challenges is associated with the use of routine histochemical techniques and immunohistochemistry, in an effort to establish standardized panels for tumour diagnosis, which could eventually reduce each analysis cost.For this purpose a simple survey was built, in which all collaborators answered questions about the markers used for carcinoma, sarcoma and round cell tumour diagnosis, as well as general questions related with the subject. We obtained twenty-one answered to the questions, from public and private laboratories.In general, in most cases immunohistochemical and histochemical methods are used for diagnosis.The wide spectrum cytokeratins are universally used to confirm carcinoma, and vimentin for sarcoma. The CD3 marker is used by all laboratories to identify T lymphocytes. For the diagnosis of B-cell lymphoma, the marker used is not consensual. In each laboratory there are different markers for more specific situations and only two labs perform PCR techniques for diagnosis. These data will be presented to promote extended discussion,namely to reach a consensus when different markers are used.