Utilização de glúten de trigo na alimentação de dourada, Sparus aurata (Linnaeus, 1758)

A incorporação de fontes proteicas alternativas à farinha de peixe na alimentação de espécies piscícolas tem sido objecto de variados estudos no âmbito da nutrição animal. Neste contexto realizou-se o presente trabalho, cujo objectivo foi determinar o nível de substituição ideal de farinha de peixe por glúten de trigo na alimentação de dourada. Foram realizados dois ensaios, um de crescimento e um de digestibilidade. O ensaio de crescimento efectuou-se para determinar qual o nível máximo de substituição sem que os animais apresentassem diferenças significativas em termos de taxa de crescimento. O ensaio de digestibilidade teve como principal objectivo determinar qual das dietas testadas proporcionaria melhores valores de utilização digestiva por parte dos animais. Tendo em conta os resultados dos ensaios e as condições experimentais em que se realizaram, verificou-se que a substituição até 40% não afecta significativamente o desempenho zootécnico das douradas. ABSTRACT; The incorporation of alternative protein sources in fish diets has been the aim of many scientific experiments in animal nutrition. ln this study, the main objective was to evaluate the ideal level of wheat gluten incorporation in substitution of fishmeal in diets for gilthead sea bream (Sparus aurata L). To reach this objective, two experiments were carried out, in which growth and digestibility were studied. The aim of the growth experiment was to evaluate the maximum level of fish meal substitution by wheat gluten (20%, 40%, 60% and 80%), without affecting significantly the animals growth rate. The digestibility experiment took place to evaluate which animals presented better food utilization with the tested diets (20%, 40%, 60% and 80%). Observing the results of these two experiments, it was concluded that the substitution until 40% did not affect significantly the performance of the fishes.

Dual layer solid phase extraction based on molecular imprinting technology: Seeking a route to enhance selectivity for trace analysis of pesticide residues in olive oil.

Aiming to introduce a multiresidue analysis for the trace detection of pesticide residues belonging to organophosphorus and triazine classes from olive oil samples, a new sample preparation methodology comprising the use of a dual layer of “tailor-made” molecularly imprinted polymers (MIPs) SPE for the simultaneous extraction of both pesticides in a single procedure has been attempted. This work has focused on the implementation of a dual MIP-layer SPE procedure (DL-MISPE) encompassing the use of two MIP layers as specific sorbents. In order to achieve higher recovery rates, the amount of MIP layers has been optimized as well as the influence of MIP packaging order. The optimized DL-MISPE approach has been used in the preconcentration of spiked organic olive oil samples with concentrations of dimethoate and terbuthylazine similar to the maximum residue limits and further quantification by HPLC. High recovery rates for dimethoate (95%) and terbuthylazine (94%) have been achieved with good accuracy and precision. Overall, this work constitutes the first attempt on the development of a dual pesticide residue methodology for the trace analysis of pesticide residues based on molecular imprinting technology. Thus, DL-MISPE constitutes a reliable, robust, and sensitive sample preparation methodology that enables preconcentration of the target pesticides in complex olive oil samples, even at levels similar to the maximum residue limits enforced by the legislation.

Espanto: Adaptação de excertos do romance "As aventuras de joão sem medo" de José Gomes Ferreira para um espectáculo de formas animadas, duas actrizes e uma máquina de "encontar"

A partir de excertos do romance "As Aventuras de João Sem Medo" (publicado em 1963) de José Gomes Ferreira (1900-1985), criou-se um espectáculo para duas actrizes manipuladoras de bonecos, interagindo com uma máquina de cena. De início, identificaram-se no romance uma série de atmosferas e pequenas unidades de acção, que serviram de base para o guião do espectáculo orientado para um público heterogéneo. Partindo dos recursos existentes, procurou-se uma dramaturgia do texto com dois níveis de representação: o jogo entre as actrizes que, enquanto fadas competem entre si pelo uso da máquina de "encontar"; e o jogo entre os bonecos que, embora autónomo do ponto de vista narrativo, acaba por funcionar como a materialização do conflito entre as manipuladoras. Do texto para o teatro, assistimos ao espanto que nos provoca uma marioneta rebelde. /ABSTRACT: From excerpts of the "The Adventures of Fearless John" a romance (published in 1963) by Jose Gomes Ferreira (1900-1985), a show was created for two actresses I puppeteers who interact with an onstage prop. From the beginning, a series of different moods and small units of action were identified, and serve as the basis for the show oriented to a general audience. Trough the existing resources, two different levels of representation were found to dramatize the text: the interplay between the actresses who, while portraying fairies, compete for the use of the storytelling box; and the interplay between the puppets who, although they are independent from the narrative point of view, end up functioning as a materialization of the conflict between the puppeteers. From the text to the theatre, we witness the wonder that a rebellious puppet inspires.

“Costa, Manuel Gomes da”

Manuel Gomes da Costa