Progress in the use of scanning electron microscopy (SEM) applied to the taxonomy of the genus Bursaphelenchus (Nematoda: Parasitaphelenchidae)

The scanning electron microscope (SEM) has been a major tool in detailed morphological observations of plant parasitic nematodes during the last 30 years, efficiently complementing light microscopical (LM) studies. Nematodes are extremely difficult to observe and characterize due to their small size (aprox. 1 mm long) and paucity of morphological characters, so detailed surface observations of several organs and nematode regions are of the highest value. Among plant parasitic nematodes, one of the most devastating species is the “pinewood nematode” (PWN), Bursaphelenchus xylophilus, which has been a major problem for forest species, and in particular pines, in Asia (Japan, China, Korea) and has been recently detected in the European Union (Portugal). B. xylophilus belongs to a closely related, morphologically similar group of species, within the genus Bursaphelenchus, and designated by the “xylophilus group”. SEM has become a crucial tool in observing several genital characters of males and females, such as male genital papillae, male copulatory spicules, female vulval flap and female genital papillae.s In this presentation, we will show how SEM has been utilized to observe and characterize the shape of the vulval flap, the presence/ absence of papillae near the flap, and confirm the presence and the arrangement of the male genital papillae. LM is also used in this work to show its value as a complementary tool to SEM, in both genital characteristics and other, general, characters of the genus Bursaphelenchus, such as the male bursa and cephalic region.

TARDI VARISCAN DEFORMATION IN THE IBERIAN PENINSULA; A LATE FEATURE IN THE LAURASIA-GONDWANA DEXTRAL COLLISION

The Late Variscan deformation event in Iberia, is characterized by an intraplate deformation regime induced by the oblique collision between Laurentia and Gondwan. This episode in Iberia is characterized by NNE-SSW strike-slip faults, which are considered by the classic works as sinistral strike-slips. However, the absence of Mesozoic formations constraining the age of this sinistral kinematics, led some authors to consider it as the result of Alpine reworking. Structural studies in Almograve and Ponta Ruiva sectors (SW Portugal), not only shows that NNE-SSW faults presents a clear sinistral kinematics and are occasionally associated with E-W dextral shears, but also that this kinematics is related to the late deformation episodes of Variscan Orogeny. In Almograve sector, the late Variscan structures are characterized by NNE-SSW sinistral kink-bands, spatially associated with E-W dextral faults. These structures are contemporaneous and affect the previously deformed Carboniferous units. The Ponta Ruiva Sector constrains the age of deformation because the E-W dextral shears affect the Late Carboniferous (late Moscovian) units, but not the overlying Triassic series. The new exposed data shows that the NNE-SSW and the E-W faults are dynamically associated and results from the same deformation event. The NNE-SSW sinistral faults could be considered as second order dominoes structures related with first order E-W dextral shears, related with Laurasia-Gondwana collision during Late Carboniferous-Permian Times.