Cante alentejano: entre o quotidiano e a patrimonialização de uma prática cultural.(O caso de Santo Aleixo da Restauração)

A presente dissertação pretende contribuir para a compreensão dos processos de patrimonialização. Especificamente, exploram-se contextos quotidianos associados à prática do Cante Alentejano, delineando as suas reconfigurações e manifestações contemporâneas. A pesquisa é ancorada metodologicamente em investigação etnográfica na localidade de Santo Aleixo da Restauração (Moura). Neste contexto empírico, caracterizam-se os espaços sociais onde é desenvolvido o quotidiano do Cante Alentejano, de forma a perceber o dualismo entre a sua prática quotidiana e aquilo que é a sua patrimonialização, tal como é percepcionada pelos atores sociais locais; Abstract: This dissertation aims to contribute to the understanding of processes of heritagization. Specifically, we explore contexts of daily practice of Cante Alentejano and identify the current manifestations and reconfigurations of this practice. Methodologically, research is based in an ethnography conducted in the locality of Santo Aleixo da Restauração (Moura). In this empirical context, we analyse daily social spaces where Cante Alentejano has been developed, with the purpose of understanding the dualism between this daily practice and the process of patrimonialization, as i tis perceived by local social actors.

Green mitigation strategy for cultural heritage: bacterial potential for biocide production

Several biosurfactants with antagonistic activity are produced by a variety of microorganisms. Lipopeptides (LPPs) produced by some Bacillus strains, including surfactin, fengycin and iturin are synthesized nonribosomally by mega-peptide synthetase (NRPS) units and they are particularly relevant as antifungal agents. Characterisation, identification and evaluation of the potentials of several bacterial isolates were undertaken in order to establish the production of active lipopeptides against biodeteriogenic fungi from heritage assets. Analysis of the iturin operon revealed four open reading frames (ORFs) with the structural organisation of the peptide synthetases. Therefore, this work adopted a molecular procedure to access antifungal potential of LPP production by Bacillus strains in order to exploit the bioactive compounds synthesis as a green natural approach to be applied in biodegraded cultural heritage context. The results reveal that the bacterial strains with higher antifungal potential exhibit the same morphological and biochemical characteristics, belonging to the genera Bacillus. On the other hand, the higher iturinic genetic expression, for Bacillus sp. 3 and Bacillus sp. 4, is in accordance with the culture antifungal spectra. Accordingly, the adopted methodology combining antifungal screening and molecular data is represent a valuable tool for quick identification of iturin-producing strains, constituting an effective approach for confirming the selection of lipopeptides producer strains.