The History of Expansion of the Genus Bursaphelenchus (Nematoda: Aphelenchida: Parasitaphelenchidae)

Because of globalization and removal of geographical barriers, frequent biological invasions of introduced species become an urgent environmental problem. According to the Convention on Biological Diversity (CBD), precise identification of dangerous aggressive species at the early stages of their invasion to new regions is the most important component of the environmental control and monitoring. To resist the potential environmental hazard, the precise data are required on the current distribution and history of expansion of pests that are of global economic importance.

O papel das estruturas lineares, artificiais e naturais, como locais de refúgio de micromamíferos

Avaliámos a importância das bermas das estradas como áreas de refúgio para pequenos mamíferos, em paisagens Mediterrânicas intensivamente pastoreadas, e comparámos esta possível função das estradas como refúgio com o papel fundamental das galerias ripícolas como reservatórios de diversidade biológica. Para esse efeito, foram realizadas capturas de micromamíferos em dois segmentos de estrada e em duas ribeiras da região de Évora. Foram capturados 457 indivíduos de cinco espécies diferentes. Mus spretus foi a espécie mais capturada, seguida de Crocidura russula e Apodemus sylvaticus. M. spretus apresentou uma maior abundância nas bermas de estrada do que na vegetação ripicola, enquanto que a abundância de C. russula e A. sylvaticus era semelhante para ambos os habitats. O número de capturas das três espécies foi bastante superior dentro dos habitats lineares do que na matriz circundante. Os indivíduos de M. spretus eram maiores nas ribeiras, mas significativamente menores fora dos habitats lineares, e os indivíduos de C. russula apresentavam uma melhor condição corporal nas bermas das estradas. Tanto as estradas como as ribeiras exerceram um forte efeito de barreira aos movimentos dos micromamíferos. Concluímos então que as bermas das estradas actuam como habitat de refúgio em áreas sub-óptimas das paisagens Mediterrânicas. ABSTRACT: We assessed the importance of road verges as refuge areas for small mammals, in highly intensified grazed pastures on a Mediterranean landscape, and compared road function as refuge with the fundamental role of riparian galleries as reservoirs of biological diversity. For this purpose, a small mammal trapping study was undertaken on road verges and on small stream sides. We sampled two road segments and two streams in the vicinity of Évora, Portugal. We captured a total of 457 individuals of five different species. Mus spretus was the most common species captured, followed by Crocidura russula and Apodemus sylvaticus. M. spretus was more abundant on road verges than on riparian strips, whilst the abundance of C. russula and A. sylvaticus were similar in the two habitats. Captures of the three species were much higher inside both linear habitats than on the surrounding matrix. M. spretus were bigger on stream sites but significantly smaller outside the linear habitats and C. russula had better body conditions on roads. 8oth roads and streams exerted a strong barrier effect to small mammals' movements. We conclude that roadside verges act as refuge habitat in sub-optimal Mediterranean landscapes.

Can birds play a role as High Nature Value indicators of montado system?

Montados form a heterogeneous landscape of wooded matrix dominated by cork and/or holm oak with open areas characterized by fuzzy boundaries. Montado supports a high biological diversity associated to low intensity management and a landscape diversity provided by a continuous gradient of land cover. Among other features this permits the classification of montados as a High Nature Value (HNV) system. We assessed the role of birds as HNV indicators for montado, and tested several bird groups—farmland, edge, forest generalists and forest specialists species; and some universal indicators such as species conservation status, Shannon’s diversity index and species richness. Our study areas covered the North–South distribution of cork oak in Portugal, and we surveyed the breeding bird communities across 117 sampling sites. In addition to variables related to management and sanitary status, we considered variables that characterize the landscape heterogeneity inside the montado—trees and shrub density and richness of woody vegetation. Our results suggest that specific bird guilds can be used as HNV indicators of particular typologies of montado, and highlight the need to develop an indicator that could be transversally applied to all types of montado.

Adventitious rooting of conifers: influence of biological factors

Vegetative propagation of superior conifer trees can be achieved e.g. through rooted cuttings or rooted microshoots, the latter predominantly through in vitro tissue culture. Both techniques are used to achieve rapid multiplication of trees with favorable genetic combinations and to capture a large proportion of the genetic diversity in a single generation cycle. However, adventitious rooting of shoots (cuttings) is often not efficient due to various problems such as scarcity of roots and cessation of their growth, both of which limit the application of vegetative propagation in some conifer species. Many factors are involved in the adventitious rooting of shoots including physical and chemical ones such as plant growth regulators, carbohydrates, light quality, temperature and rooting substrates or media (reviewed by Ragonezi et al. 2010). The focus of this review is on biological factors, such as inoculations with Agrobacterium rhizogenes, plant- growth-promoting rhizobacteria and other endophytes, and mycorrhizal fungi, which were found to stimulate adventitious rooting. These microorganisms could contribute not only to adventitious root development but also help in protecting conifer plants against pathogenic microorganisms, facilitate acclimation and transplanting, and contribute to more sustainable, chemical-free forests.

Development of molecular approaches to assess the diversity and abundance of marine nematodes assemblages for assessment of Good Environmental Status of the estuarine and marine ecosystems

In Europe, the concerns with the status of marine ecosystems have increased, and the Marine Directive has as main goal the achievement of Good Environmental Status (GES) of EU marine waters by 2020. Molecular tools are seen as promising and emerging approaches to improve ecosystem monitoring, and have led ecology into a new era, representing perhaps the most source of innovation in marine monitoring techniques. Benthic nematodes are considered ideal organisms to be used as biological indicator of natural and anthropogenic disturbances in aquatic ecosystems underpinning monitoring programmes on the ecological quality of marine ecosystems, very useful to assess the GES of the marine environment. dT-RFLP (directed Terminal-Restriction Fragment Length Polymorphism) allows to assess the diversity of nematode communities, but also allows studying the functioning of the ecosystem, and combined with relative real-time PCR (qPCR), provides a high-throughput semi-quantitative characterization of nematode communities. These characteristics make the two molecular tools good descriptors for the good environmental status assessment. The main aim of this study is to develop and optimize the dT-RFLP and qPCR in Mira estuary (SW coast, Portugal). A molecular phylogenetic analysis of marine and estuarine nematodes is being performed combining morphological and molecular analysis to evaluate the diversity of free-living marine nematodes in Mira estuary. After morphological identification, barcoding of 18S rDNA and COI genes are being determined for each nematode species morphologically identified. So far we generated 40 new sequences belonging to 32 different genus and 17 families, and the study has shown a good degree of concordance between traditional morphology-based identification and DNA sequences. These results will improve the assessment of marine nematode diversity and contribute to a more robust nematode taxonomy. The DNA sequences are being used to develop the dT-RFLP with the ability to easily process large sample numbers (hundreds and thousands), rather than typical of classical taxonomic or low throughput molecular analyses. A preliminary study showed that the digest enzymes used in dT-RFLP for terrestrial assemblages separated poorly the marine nematodes at taxonomic level for functional group analysis. A new digest combination was designed using the software tool DRAT (Directed Terminal Restriction Analysis Tool) to distinguished marine nematode taxa. Several solutions were provided by DRAT and tested empirically to select the solution that cuts most efficiently. A combination of three enzymes and a single digest showed to be the best solution to separate the different clusters. Parallel to this, another tool is being developed to estimate the population size (qPCR). An improvement in qPCR estimation of gene copy number using an artificial reference is being performed for marine nematodes communities to quantify the abundance. Once developed, it is proposed to validate both methodologies by determining the spatial and temporal variability of benthic nematodes assemblages across different environments. The application of these high-throughput molecular approaches for benthic nematodes will improve sample throughput and their implementation more efficient and faster as indicator of ecological status of marine ecosystems.