Identification of endophytic bactéria from micropropagated yacon (Smallanthus sonchifolius) crowing under autotrophic and Heterotrophic systems.

Yacon, Smallanthus sonchifolius, an Andean species. is a rich source of dictetíc oligofructans with low glucose content. proteins and phenolic compounds. These constituents have shown efficacy in the prevention of diet-related ehronic diseases, including gastroin-testinal disorders and diabetes |1,2|. Yacon is part of a research program at the National Center for Natural Products Research (NCNPR) and University of Mississippi Field Station to develop new alternative root crops for Mississippi while attempting to im-prove the diet of low incorne families. Yacon can be easily propa-gated by cultings. Virus and nematode infections have been re-ported on plants propagated by cuttings in Brazil. a country that hás adopted Yacon as specialty crop [3|. We have developed two culture systems. autotrophic and heterotrophic, to produce healthy plants. Herem we describe the presence of endophytic bactéria m micropropagated Yacon. In auxin free media, new roots were induced. Overa 15day period. the average root mduction per expiam was 5.45 to 8.75 under autotrophic and heterotrophic cul-tures, respectively. Root lenglh vaned between 3 and 60mrn. The presence of root hairs and lateral roots was noticed only in auto-trophic condilions. These beneficiai bactéria were identified and chemically ctiaracterized. Acknowledgement: This research work was partially supported by the USDA/ARS Cooperative Research Agreement No. 58-6408-2-009. Referentes; |1) Terada S. et ai. (2006] Yakugaku Zasshi 126(8): 665-669. (2| Valentová K. Ulri-chová j. (2003) Biomedical Papers 147: 119-130. [3| Mogor C. et ai, (2003) Acta Horticulturea 597: 311 -313.

Flowering synchronization in hybrid rice parental lines.

The cultivation of hybrid rice is a technology that allows for an increase in grain yield of 30% relative to the grain yield of conventional cultivars. However, the main challenge for this technology is related to seed production, which has high production costs and low seed yields. Therefore, agronomic techniques that could enhance flowering synchrony of parental lines in the field are essential for an efficient production system of hybrid rice seeds. The objective of this work was to study the effects of sowing depth, plant density and fertilization with nitrogen or phosphorus as potential techniques to increase the pollen availability in the field and, consequently, the flowering synchrony between parental lines in the production of hybrid rice seeds. The experiments were conducted during two growing seasons in the Central Region of Brazil. All of the experiments were conducted as a randomized complete block in a split plot scheme; however, the experiment with P fertilization had a factorial design. Our research allow inferring that nitrogen fertilization technique applied to the soil or foliar at the time of panicle differentiation does not affect the time of onset of flowering of rice varieties INTA Puitá CL and L106R, which are potential R lines for the production of hybrid rice. Agronomic techniques of variation in sowing depth, seeding rate and the phosphate fertilization affect the time of onset of flowering from 10 to 19 degree-days, which could represent two days in the crop cycle, for the line L106R. Such techniques constitute potential alternatives for use in hybrid rice seed production systems and could be applied in alternated blocks of R lines in the field to obtain longer periods of pollen availability in the field.