Genome selection in fruit breeding: application to table grapes.

Genomic selection (GS) has recently been proposed as a new selection strategy which represents an innovative paradigm in crop improvement, now widely adopted in animal breeding. Genomic selection relies on phenotyping and high-density genotyping of a sufficiently large and representative sample of the target breeding population, so that the majority of loci that regulate a quantitative trait are in linkage disequilibrium with one or more molecular markers and can thus be captured by selection. In this study we address genomic selection in a practical fruit breeding context applying it to a breeding population of table grape obtained from a cross between the hybrid genotype D8909-15 (Vitis rupestris × Vitis arizonica/girdiana), which is resistant to dagger nematode and Pierce?s disease (PD), and ?B90-116?, a susceptible Vitis vinifera cultivar with desirable fruit characteristics. Our aim was to enhance the knowledge on the genomic variation of agronomical traits in table grape populations for future use in marker-assisted selection (MAS) and GS, by discovering a set of molecular markers associated with genomic regions involved in this variation. A number of Quantitative Trait Loci (QTL) were discovered but this method is inaccurate and the genetic architecture of the studied population was better captured by the BLasso method of genomic selection, which allowed for efficient inference about the genetic contribution of the various marker loci. The technology of genomic selection afforded greater efficiency than QTL analysis and can be very useful in speeding up the selection procedures for agronomic traits in table grapes.

Marker-assisted selection integrated to the embrapa common bean breeding program.

The establishment of a specific Marker-Assisted Selection Facility at the Embrapa Rice and Beans Biotechnology Laboratory, in 2014, has better supported the routine analysis with molecular markers demanded by the Embrapa Common Bean Breeding Program. In addition, it has also supported other Embrapa plant breeding programs, such as rice and cotton.

Triticale breeding program for fusarium head blight resistance.

2016

Use of doubled haploid lines via isolated microspore culture for the introgression of resistance to Fusarium head blight into wheat elite cultivars.

2016

Estimates of genetic parameters using RELM/BLUP for intrapopulational genetic breeding of Platonia insignis Mart.

The aim of this study was to estimate genetic parameters to support the selection of bacuri progenies for a first cycle of recurrent selection, using the REML/BLUP (restricted maximum likelihood/best linear unbiased prediction) procedure to estimate the variance components and genotypic values. Were evaluated twelve variables in a total of 210 fruits from 39 different seed trees, from a field trial with an experimental design of incomplete blocks with clonal replies among subplots. The three variables related with the fruit development (weight, diameter, length) showed strong correlation, and where fruit length showed higher heritability and potential to be used for indirect selection. Among the 39 progenies evaluated in this study, five present potential to compose the next cycle of recurrent selection, due they hold good selection differential either to agrotechnological variables as to development of bacuri fruit.

Selection of mango rosa genotypes in a breeding population using the multivariate-biplot method.

Mango (Mangifera indica L.) trees stand out among the main fruit trees cultivated in Brazil. The mango rosa fruit is a very popular local variety (landrace), especially because of their superior technological characteristics such as high contents of Vitamin C and soluble solids (SS), as well as attractive taste and color. The objective of this study was to select a breeding population of mango rosa (polyclonal variety; ≥5 individuals) that can simultaneously meet the fresh and processed fruit Vmarkets, using the multivariate method of principal components and the biplot graphic.

Recovery of reproductive activity and fertility of Saanen goats affected by hydrometra after cloprostenol treatment and estrus induction during the non-breeding season (preliminary data).

2016

Grape breeding and genetic studies in Brazil.

2012

Interspecific hybrids between Paspalum plicatulum and P. oteroi: a key tool for forage breeding.

2016

Breeding calabrian pepper lines (capsicum annuum L.) for Brazilian agriculture from sui generis introduction of germplasm.

This work aimed to report on the introduction of germplasm in a sui generis way and the initial results of Calabrian pepper breeding at Embrapa Vegetables.