Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion.

Twenty endophytic bacteria were isolated from the meristematic tissues of three varieties of strawberry cultivated in vitro, and further identified, by FAME profile, into the genera Bacillus and Sphingopyxis. The strains were also characterized according to indole acetic acid production, phosphate solubilization and potential for plant growth promotion. Results showed that 15 strains produced high levels of IAA and all 20 showed potential for solubilizing inorganic phosphate. Plant growth promotion evaluated under greenhouse conditions revealed the ability of the strains to enhance the root number, length and dry weight and also the leaf number, petiole length and dry weight of the aerial portion. Seven Bacillus spp. strains promoted root development and one strain of Sphingopyxis sp. promoted the development of plant shoots. The plant growth promotion showed to be correlated to IAA production and phosphate solubilization. The data also suggested that bacterial effects could potentially be harnessed to promote plant growth during seedling acclimatization in strawberry

Desempenho agronômico de linhas de melancia com resistência ao oídio.

O objetivo deste trabalho foi avaliar o desempenho agronômico em termos de produtividade e características de frutos em 32 genótipos de melancia. O ensaio foi conduzido na Embrapa Semi-Árido, em Petrolina-PE, durante o período de agosto a outubro de 2005, utilizando o delineamento experimental de blocos ao acaso, com quatro repetições e parcelas com 10 plantas. As mudas foram transplantadas para o campo no espaçamento de 3 x 0,8 m. A cv ?C. Sweet? e as 31 linhas originadas do cruzamento entre ?C. Sweet? e ?CPATSA 85-030? foram avaliadas quanto a rendimento e aspectos externos e internos dos frutos. Verificou-se alto rendimento dos genótipos (33,6 a 66,7 t/ha). Na maioria das linhas, predominaram os frutos com casca rajada, com listras verde-claras alternadas com verde-escuras (96,8%), polpa vermelha (83,9%), alto teor de sólidos solúveis (10 a 12,3o Brix) (93,5%), frutos grandes (8,60 a 11,70 kg) (54,8%) e formato arredondado. Algumas linhas (9,7%) apresentaram alta prolificidade (2,9 a 2,5 frutos/planta), frutos pequenos e arredondados (4,30 a 4,60 kg) e casca rajada verde escura (3,2%). Entre os genótipos de melancia, 05.1168.003, 05.1172.004, 05.1185.001, 05.1189.003, 05.1203.007/1, 05.1194.001/1, 05.1194.005 e 05.1194.006 foram os mais promissores para a obtenção de cultivares resistentes ao oídio e com padrões de frutos para os mercados interno e externo.

Porta-enxertos clonais para pessegueiro: estabelecimento da unidade de observação com a cultivar maciel na colônia são Manoel, em Pelotas-RS.

2015

Identification and effects of mixed infection of Potyvirus isolates with Cucumber mosaic virus in cucurbits.

Mixed infections in cucurbits are frequently observed in natural conditions between viruses from the Potyvirus genus and Cucumber mosaic virus (CMV), which significantly decreases productivity. The objectives of the present study was to compare the host range of PRSV-W, WMV, and ZYMV isolates and evaluate the effects of mixed infections with CMV in zucchini plants (Cucurbita pepo L.). Host range studies comprising 23 plant species confirmed some similarities and biological differences among the isolates of PRSV-W, ZYMV, and WMV. RT-PCR confirmed the amplification of DNA fragments of the PRSV-W, WMV, and ZYMV coat protein gene (cp) and cytoplasm inclusion gene (ci). The virus interaction studies in zucchini Caserta plants indicated synergistic interactions, particularly among species from the Potyvirus genus, and some CMV interference with some virus combinations.