The infection of soybean leaves by Phakopsora pachyrhizi during conditions of discontinuous wetness.

The ability of Phakopsora pachyrhizi to cause infection under conditions of discontinuous wetness was investigated. In in vitro experiments, droplets of a uredospore suspension were deposited onto the surface of polystyrene. After an initial wetting period of either 1, 2 or 4 h, the drops were dried for different time intervals and then the wetness was restored for 11, 10 or 8 h. Germination and appressorium formation were evaluated. In in vivo experiments, soybean plants were inoculated with a uredospore suspension. Leaf wetness was interrupted for 1, 3 or 6 h after initial wetting periods of 1, 2 or 4 h. Then, the wetting was re-established for 11, 10 or 8 h, respectively. Rust severity was evaluated 14 days after inoculation. The germination of the spores and the formation of the appressoria on the soybean leaves after different periods of wetness were also quantified in vivo by scanning electron microscopy. P. pachyrhizi showed a high infective capacity during short periods of time. An interruption of wetness after 1 h caused average reductions in germination from 56 to 75% and in appressorium formation from 84 to 96%. Rust severity was lower in all of the in vivo treatments with discontinuous wetness when compared to the control plants. Rust severity was zero when the interruption of wetness occurred 4 h after the initial wetting. Wetting interruptions after 1 and 2 h reduced the average rust severity by 83 and 77%, respectively. The germination of the uredospores on the soybean leaves occurred after 2 h of wetness, with a maximum germination appearing after 4 h of wetness. Wetness interruption affected mainly the spores that had initiated the germination.

Biophysical indicators based on satellite images in an irrigated area at the São Francisco River Basin, Brazil.

The Jaíba Irrigated Perimeter is a large irrigated agriculturearea, located in the region Forest Jaíba between the SãoFrancisco and Verde Grande rivers, in the Brazilian semi-arid region. In 2014, irrigators thisthe region face losses in theinterruption of new plantings in irrigated areas due to water scarcity. The objective ofthis study is combine the modelto estimate the Monteith BIO with the SAFER algorithm in the case of obtaining ET, to analyze the dynamics of naturalvegetation and irrigated crops in water scarcity period. For application of the model are necessary data frommeteorological stations and satellite images. Were used 23satellite images of MODIS withspatial resolution of 250mand temporal 16 days, of 2014 year. For analyze the results,we used central pivots irrigation mask of Minas Geraisstate, Brazil. In areas with irrigated agriculture with central pivot, the mean values of BIO over the year 2014 were88.96 kg.ha-1.d-1. The highest values occurred between April 23 and May 8, with BIO 139 kg.ha-1.d-1. For areas withnatural vegetation, the average BIO was 88.34 kg.ha-1.d-1with lower values in September. Estimates of ET varied withthe lowest values of ET observedin natural vegetation 1,91±1,22 mm.d-1and the highest values in irrigated area isobserved 3,51±0,97 mm.d-1. Results of this study can assist in monitoring of river basins, contributing to themanagement irrigated agriculture, with the trend of scarcity of water resources and increasing conflicts for the wateruse.