1 resultado para medium-range order
em Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa)
Filtro por publicador
- Aberystwyth University Repository - Reino Unido (4)
- Academic Archive On-line (Jönköping University; Sweden) (1)
- Academic Research Repository at Institute of Developing Economies (3)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (11)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (4)
- Aquatic Commons (2)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (17)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (9)
- Aston University Research Archive (44)
- Biblioteca de Teses e Dissertações da USP (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (23)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (16)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (28)
- Boston University Digital Common (1)
- Brock University, Canada (4)
- CaltechTHESIS (15)
- Cambridge University Engineering Department Publications Database (12)
- CentAUR: Central Archive University of Reading - UK (136)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (50)
- Cochin University of Science & Technology (CUSAT), India (5)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (7)
- CORA - Cork Open Research Archive - University College Cork - Ireland (7)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (38)
- Digital Commons - Michigan Tech (3)
- Digital Commons - Montana Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (6)
- DigitalCommons - The University of Maine Research (3)
- DigitalCommons@The Texas Medical Center (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (1)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Glasgow Theses Service (1)
- Helda - Digital Repository of University of Helsinki (5)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (2)
- Indian Institute of Science - Bangalore - Índia (77)
- Instituto Gulbenkian de Ciência (1)
- Instituto Politécnico do Porto, Portugal (4)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- National Center for Biotechnology Information - NCBI (6)
- Nottingham eTheses (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (4)
- Publishing Network for Geoscientific & Environmental Data (29)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (32)
- Queensland University of Technology - ePrints Archive (45)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico da Universidade de Évora - Portugal (2)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (8)
- Repositório Institucional da Universidade de Brasília (4)
- Repositório Institucional da Universidade Federal do Rio Grande - FURG (2)
- Repositório Institucional da Universidade Tecnológica Federal do Paraná (RIUT) (1)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (123)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- Universidad de Alicante (10)
- Universidad del Rosario, Colombia (1)
- Universidad Politécnica de Madrid (41)
- Universidade Complutense de Madrid (3)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade Federal do Pará (3)
- Universidade Federal do Rio Grande do Norte (UFRN) (4)
- Universita di Parma (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (2)
- Université de Montréal (1)
- Université de Montréal, Canada (9)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (3)
- University of Queensland eSpace - Australia (6)
- University of Washington (2)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Background:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 μ M 2,4-D +0.44 μ M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 μ M 2,4-D + 0.44 μ MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used.