Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).

2014

Nomuraea rileyi (Hypocreales: Clavicipitaceae) in Helicoverpa armigera (Lepidoptera: Noctuidae) Larvae in Brazil.

2015

Farm water productivity in broiler production: case studies in Brazil.

2016

Identification of common bean resistant sources to angular leaf spot disease in a brazilian germplasm collection.

The aim of this study was to identify sources of resistance in the germplasm collection providing information of potential sources of resistance to introduce in breeding programs.

Reaction of common bean lines to Pseudocercospora griseola in different enviromental conditions.

This study aimed to compare the reaction of common bean lines to Pseudocercospora griseola in different enviromental conditions.

Genome-wide copy number variation (CNV) detection in Nelore cattle reveals highly frequent variants in genome regions harboring QTLs affecting production traits.

Background: Copy number variations (CNVs) have been shown to account for substantial portions of observed genomic variation and have been associated with qualitative and quantitative traits and the onset of disease in a number of species. Information from high-resolution studies to detect, characterize and estimate population-specific variant frequencies will facilitate the incorporation of CNVs in genomic studies to identify genes affecting traits of importance. Results: Genome-wide CNVs were detected in high-density single nucleotide polymorphism (SNP) genotyping data from 1,717 Nelore (Bos indicus) cattle, and in NGS data from eight key ancestral bulls. A total of 68,007 and 12,786 distinct CNVs were observed, respectively. Cross-comparisons of results obtained for the eight resequenced animals revealed that 92 % of the CNVs were observed in both datasets, while 62 % of all detected CNVs were observed to overlap with previously validated cattle copy number variant regions (CNVRs). Observed CNVs were used for obtaining breed-specific CNV frequencies and identification of CNVRs, which were subsequently used for gene annotation. A total of 688 of the detected CNVRs were observed to overlap with 286 non-redundant QTLs associated with important production traits in cattle. All of 34 CNVs previously reported to be associated with milk production traits in Holsteins were also observed in Nelore cattle. Comparisons of estimated frequencies of these CNVs in the two breeds revealed 14, 13, 6 and 14 regions in high (>20 %), low (<20 %) and divergent (NEL > HOL, NEL < HOL) frequencies, respectively. Conclusions: Obtained results significantly enriched the bovine CNV map and enabled the identification of variants that are potentially associated with traits under selection in Nelore cattle, particularly in genome regions harboring QTLs affecting production traits.

Juvenile-mature genetic correlations in Pinus taeda clones propagated via somatic embryogenesis.

Objetivou-se com o presente trabalho, estimar a correlação genética entre idades de seleção (juvenil-adulta) e eficiência da seleção precoce para as características altura, diâmetro e volume de indivíduos de famílias de Pinus taeda propagados via embriogênese somática. O estudo foi realizado por meio de análise genético-estatística pelo procedimento de estimação de componentes de variância (Reml) e de predição de valores genéticos (Blup), usando-se o software Selegen-Reml/Blup. As correlações genéticas entre idades juvenis e idade de rotação foram realizadas aplicando o modelo linear desenvolvido por Lambeth (1980). Segundo os resultados do modelo estabelecido, a seleção precoce pode ser realizada em clones de Pinus taeda com alta eficiência de seleção. As idades de 4 a 6 anos são suficientes para selecionar clones de Pinus taeda propagados via embriogênese somática para colheita aos 8 e 12 anos e, as idades de 6 a 10 anos são suficientes para selecionar para colheita aos 20 anos. De acordo com as estimativas de correlação genotípicaa partir dos ambientes, a seleção de clones de Pinus taeda propagados via embriogênese somática deve ser praticada de forma específica para cada ambiente. Pode-se realizar a seleção de clones considerando o diâmetro, visto a alta correlação observada entre volume e diâmetro.

Internal Temperature of Girolando (Holstein×Gir) heifers in integrated crop, livestock (ICLS) and forestry (ICLFS) systems.

The objective of this work was to evaluate the internal temperature (IT) of Girolando heifers in integrated crop, livestock (ICLS) and forestry (ICLFS) systems. Trial was carried out at experimental field of Embrapa Rondônia, Porto Velho, Rondônia, Brazil.

Milk composition of Murrah buffalo cows supplemented with cupuassu byproduct in Western Amazon.

The aim of this study was to evaluate milk composition of Murrah buffalo cows supplemented with rations containing cupuassu byproduct in substitution of ground corn. Isoprotein and isoenergy rations were balanced with 22.0% of crude protein (CP) and 80.1% of total digestible nutrient (TDN).

Cupuassu byproduct in supplements for buffalo cows: milk yield and calf performance.

The aim of this study was to evaluate milk yield and calf performances of Murrah buffalo cows supplemented with rations containing cupuassu byproduct in substitution of ground corn.

Detection of Rupestris stem pitting associated virus in seedlings of virus - infected maternal grapevine plants.

Rupestris stem pitting associated virus (RSPaV) is a species in the genus Foveavirus (Martelli and Jelkman, 1998) and the family Flexiviridae. The virion has a positive sense, single stranded, polyadenylated RNA genome of 8.7kb in size and a coat protein of 28kD (Martelli and Jelkman, 1998). The virus has been reported to be present in pollen (Rowhani et aI., 2000) and seeds (Stewart and Nassuth, 2001), however, it has not been proved to be seed-transmitted. In our investigation reported here we have proven that RSPaV transmits by seed from RSPaV-infected mother plants to their siblings.

Sorological evaluation of commercial vaccines against enterotoxemia in goats.

Enterotoxemia in sheep and in goats is caused by the effects of the epsilon toxin of C/ostridium perfringens type D, being considered the main infectious cause of mortality in those animal species. The main prophylactic measures include adequate nutritional management and vaccination of ali animais using vaccines of high immunogenic power. Six commercial vaccines containing in its formulation the epsilon toxoid of C. perfringens type D were sorogically evaluated. Eighty four female goat kids, whose mothers had no previous vaccination history against clostridioses were used. They were divided into six groups of 14 animais each. The animais of the control group didn't receive any vaccine dose and the animais from the groups 1 to 5 received two vaccine doses, The first vaccine dose was applied at 45 days of life (day zero) and the second dose at 75 days (30 days after the first dose). Blood samples were collected from the goat kids at the days zero, 30, 60, 90, 120 and 150 after the beginning of the experiment, in order to evaluate the immunologic response. The Indirect ELlSA technique was used for the quantification of the antibodies against epsilon toxin in the samples of blood serum of the animais. In day zero, no animal presented titre considered protector. The largest number of animais considered protected was found at day 60, in response to the two initial doses of the vaccine (days O and 30, first and second doses, respectively). Only tive animaIs which received the vaccine 1 and one animal which received the vaccine 3 stayed wilh titres of antibodies considered up to 150 days after the first vaccine dose. Based on the results, it was concluded lhat the evaluated vaccines showed small amount of epsilon toxoid in the commercial formulations, a crucial fact for lhe low efficiency of. the vaccines. For commercial reasons, the vaccines against the clostridioses present versatile formulations, with several toxoid types, used for various animal species, which certainly contributed to reduce their effectiveness in preventing the iIInesses caused by the clostridia or their toxins.

AGRIFIS - simulador de prospecção de cenários e avaliação de projetos de produção de sementes, óleo in natura e biodiesel de mamona.

Este trabalho propõe um modelo de prospecção de cenários e avaliação de projetos de atividades econômicas de produção agrícola de mamona caracterizada como agricultura familiar, extração de óleo vegetal in natura de sementes de mamona e produção de biodiesel (transesterificação), na forma de cadeia produtiva integrada. O modelo utiliza indicadores de avaliação econômico-flnanceiro, ambiental e social, como por exemplo, VPL, TIR, PBS, PBD, custo de produção, renda familiar, número de famílias assentadas e funcionários empregados, e outros, para identificar o desempenho de cada proposta de projeto. Para tanto, foi desenvolvido um ambiente computacional "AGRIFIS" para abrigar o modelo proposto nesse trabalho, que é uma ferramenta de apoio à tomada de decisão para investimentos em cadeia produtiva de mamona, pois se pode estimar o fluxo de caixa futuro de uma determinada proposta de projeto, incluindo as lucratividades, as despesas, a rentabilidade das famílias assentadas para determinadas condições pré-estabelecidas.

Water risk contamination trends by yellow-passion fruit pesticides in a small catchment in the eastern Amazon.

2009

Trends in vertical movement of hexazinone herbicide considering typic eutrorthox soil of Espraiado´s stream cultivated with sugar-cane crop - local and default data scenarios.

2009