Use of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil

Brazil is one the largest producers and exporters of food commodities in the world. The evaluation of fungi capable of spoilage and the production mycotoxins in these commodities is an important issue that can be of help in bioeconomic development. The present work aimed to identify fungi of the genus Aspergillus section Flavi isolated from different food commodities in Brazil. Thirty-five fungal isolates belonging to the section Flavi were identified and characterised. Different classic phenotypic and genotypic methodologies were used, as well as a novel approach based on proteomic profiles produced by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Type or reference strains for each taxonomic group were included in this study. Three isolates that presented discordant identification patterns were further analysed using the internal transcribed spacer (ITS) region and calmodulin gene sequences. The data obtained from the phenotypic and spectral analyses divide the isolates into three groups, corresponding to taxa closely related to Aspergillus flavus, Aspergillus parasiticus, and Aspergillus tamarii. Final polyphasic fungal identification was achieved by joining data from molecular analyses, classical morphology, and biochemical and proteomic profiles generated by MALDI-TOF MS.

Further results on the inverse along an element in semigroups and rings

In this paper, we introduce a new notion in a semigroup $S$ as an extension of Mary's inverse. Let $a,d\in S$. An element $a$ is called left (resp. right) invertible along $d$ if there exists $b\in S$ such that $bad=d$ (resp. $dab=b$) and $b\leq_\mathcal{L}d$ (resp. $b\leq_\mathcal{R}d$). An existence criterion of this type inverse is derived. Moreover, several characterizations of left (right) regularity, left (right) $\pi$-regularity and left (right) $*$-regularity are given in a semigroup. Further, another existence criterion of this type inverse is given by means of a left (right) invertibility of certain elements in a ring. Finally we study the (left, right) inverse along a product in a ring, and, as an application, Mary's inverse along a matrix is expressed.

The inverse along a product and its applications

In this paper, we study the recently defined notion of the inverse along an element. An existence criterion for the inverse along a product is given in a ring. As applications, we present the equivalent conditions for the existence and expressions of the inverse along a matrix.

Percursos formativos e transições profissionais: o caso dos diplomados de um mestrado na área dos recursos humanos

Publicado em "40 anos de democracia(s): progressos, contradições e prospetivas: atas do VIII Congresso Português de Sociologia". ISBN 978-989-97981-2-0

Relatório de atividade profissional

Dissertação de mestrado em Ciências - Formação Contínua de Professores (área de especialização em Biologia e Geologia)

A supervisão pedagógica como prática de transformação: o lugar das narrativas profissionais

Este texto tem como objetivo discutir o lugar das narrativas profissionais, de natureza autobiográfica, produzidas por professores em contexto de formação pós-graduada em supervisão pedagógica. Começando pela discussão do conceito de qualidade em educação, e que radica numa concepção de educação para o desenvolvimento sustentável e para a transformação de pessoas e de contextos, a autora passa para uma análise do papel dos professores no desenvolvimento de práticas pedagógicas de qualidade. Embora os contextos de educação e de formação tenham um papel determinante, os professores, principalmente através das expectativas que detêm face aos seus alunos, têm um papel crucial no combate a formas de discriminação e de exclusão nas escolas. O conceito de supervisão que informa este texto também se coloca ao serviço da qualidade em educação enunciada, ao ser tomado enquanto teoria e prática de regulação crítica e colaborativa da pedagogia e do desenvolvimento profissional, numa visão da educação para a transformação e para a sustentabilidade. O texto finaliza com a ilustração do modo como as narrativas profissionais se constituem enquanto estratégias de autossupervisão do trabalho docente num contexto de formação pós-graduada. Desenvolvidas em contextos profissionais, e estabelecendo pontes entre o conhecimento acadêmico e experiencial, elas incidem na análise dos modos como o trabalho docente se coloca (ou não) ao serviço de uma pedagogia e de um desenvolvimento profissional docente assente em valores de sustentabilidade e de transformação, identificando os constrangimentos estruturais e apontando rumos de ação.

Dano reflexo nos acidentes de viação

Relatório de atividade profissional de mestrado em Direito dos Contratos e da Empresa

Formação de professores: uma prática pedagógica frente aos desafios das necessidades educativas especiais: um estudo de caso numa IES Particular em Manaus-Am

Dissertação de mestrado em Educação Especial (área de especialização em Dificuldades de Aprendizagem Específicas)

Cutinase promotes dry esterification of cotton cellulose

Cutinase from Thermobifida fusca was used to esterify the hydroxyl groups of cellulose with the fatty acids from triolein. Cutinase and triolein were pre-adsorbed on cotton and the reaction proceeded in a dry state during 48 hours at 35ºC. The cutinase-catalyzed esterification of the surface of cotton fabric resulted in the linkage of the oleate groups to the glycoside units of cotton cellulose. The superficial modification was confirmed by performing ATR-FTIR on treated cotton samples and by MALDI-TOF analysis of the liquors from the treatment of the esterified cotton with a crude cellulase mixture. Modified cotton fabric also showed a significant increase of hydrophobicity. This work proposes a novel bio-based approach to obtain hydrophobic cotton. This article is protected by copyright. All rights reserved.

Dislexia: conhecimentos de educadores e professores do 1º ciclo do ensino básico na Zona Norte de Portugal

Dissertação de mestrado em Educação Especial (área de especialização em Dificuldades de Aprendizagem Específicas)

A produção de espetáculo e a formação de públicos na Companhia de Teatro de Braga

Projeto de mestrado em Mediação Cultural e Literária

Enzymatic hydrophobic modification of jute fibers via grafting to reinforce composites

Horseradish peroxidase (HRP)/H2O2 system catalyzes the free-radical polymerization of aromatic compounds such as lignins and gallate esters. In this work, dodecyl gallate (DG) was grafted onto the surfaces of lignin-rich jute fabrics by HRP-mediated oxidative polymerization with an aim to enhance the hydrophobicity of the fibers. The DG-grafted jute fibers and reaction products of their model compounds were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The results clearly indicated the grafting of DG to the jute fiber by HRP. Furthermore, the hydrophobicity of jute fabrics was determined by measuring the wetting time and static contact angle. Compared to the control sample, the wetting time and static contact angle of the grated fabrics changed from ~1 s to 1 h and from ~0° to 123.68°, respectively. This clearly proved that the hydrophobicity of jute fabrics improved considerably. Conditions of the HRP-catalyzed DG-grafting reactions were optimized in terms of the DG content of modified jute fabrics. Moreover, the results of breaking strength and elongation of DG-grafted jute/ polypropylene (PP) composites demonstrated improved reinforcement of the composite due to enzymatic hydrophobic modification of jute fibers.