34 resultados para Nanoparticulas de dióxido de titânio e prata
em Universidade do Minho
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Dissertação de mestrado integrado em Engenharia de Materiais
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Dissertação de mestrado em Engenharia de Materiais
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Dissertação de mestrado integrado em Engenharia de Materiais
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Tese de Doutoramento em Engenharia Eletrónica e de Computadores.
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Dissertação de mestrado integrado em Engenharia Civil
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Dissertação de mestrado integrado em Engenharia Biomédica
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Dissertação de mestrado integrado em Engenharia de Materiais
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Tese de Doutoramento em Biologia de Plantas
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Tese de Doutoramento (Programa Doutoral em Engenharia de Materiais)
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Cell Sheets of hASCs (hASCs-CS) have been previously proposed for wound healing applications(1, 2) and despite the concern for production time reduction, the possibility of having these hASCs-CS off-the-shelf is appealing. The goal of this work was to define a cryopreservation methodology allowing to preserve cells viability and the properties CS matrix. hASCs-CS obtained from three different donors were created in UP-cell thermoresponsive dishes(Nunc, Germany) as previously reported(1,2). Different cryopreservation conditions were considered: i)FBS plus DMSO(5% and10%); ii)0.4M of Trehalose plus DMSO (5% and 10%); iii)cryosolution PLL (Akron Biotech, USA); and iv)vitrification. The cryopreservation effect was first assessed for cellular viability by flow cytometry using 7-AAD, and after dissociating the hASCs-CS with collagenase and trypsin-EDTA 0.25%. The expression (RT-PCR) and deposition (western blot and immunocytochemistry) of collagen type I, laminin and fibronectin, and the organization (TEM) of the extracellular matrix was further assessed before and after hASCs-CS cryopreservation to determine a potential effect of the method over matrix composition and integrity. The obtained results confirmed that cell viability is affected by the cryopreservation methodology, as shown before for different CS(3). Interestingly, the matrix properties were not significantly altered and the typical cell sheetâ s easiness of manipulation for transplantation was not lost.
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PhD thesis in Biomedical Engineering
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Dissertação de mestrado integrado em Engenharia Mecânica
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Tese de Doutoramento Biologia Molecular e Ambiental - Especialidade em Biologia Celular e Saúde
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[Excerto] Incluído numa prática salutar de pesquisa que nos últimos anos tem unido pesquisadores brasileiros e portugueses em torno de temáticas da área da Comunicação, o livro organizado por Madalena Oliveira e Nair Prata coloca o rádio como centro de reflexão oriunda dos estudos realizados nos dois países. Enfocando a trajetória e os cenários do rádio no Brasil e em Portugal, entretanto, procura realizar um estudo que foge, estrito senso, às perspectivas de algumas pesquisas comparadas que particularizam aspectos relevantes e destoantes do objeto foco da reflexão. Como um primeiro movimento, antes da realização de pesquisas comparadas em novas bases metodológicas, tal como é especificado na Nota de Apresentação, esta obra objetiva produzir inicialmente uma cartografia do rádio, a partir dos resultados de pesquisas e reflexões de pesquisadores inseridos na comunidade lusófona. O esforço, cujos resultados são primorosos, resultou da articulação política possibilitada pela existência do Grupo de Rádio e Mídia Sonora da Sociedade de Estudos Interdisciplinares de Comunicação – Intercom (Brasil) e do Grupo de Rádio e Meios Sonoros da Associação Portuguesa de Ciências da Comunicação – Sopcom (Portugal).
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The Great Lakes lie within a region of East Africa with very high human genetic diversity, home of many ethno-linguistic groups usually assumed to be the product of a small number of major dispersals. However, our knowledge of these dispersals relies primarily on the inferences of historical, linguistics and oral traditions, with attempts to match up the archaeological evidence where possible. This is an obvious area to which archaeogenetics can contribute, yet Uganda, at the heart of these developments, has not been studied for mitochondrial DNA (mtDNA) variation. Here, we compare mtDNA lineages at this putative genetic crossroads across 409 representatives of the major language groups: Bantu speakers and Eastern and Western Nilotic speakers. We show that Uganda harbours one of the highest mtDNA diversities within and between linguistic groups, with the various groups significantly differentiated from each other. Despite an inferred linguistic origin in South Sudan, the data from the two Nilotic-speaking groups point to a much more complex history, involving not only possible dispersals from Sudan and the Horn but also large-scale assimilation of autochthonous lineages within East Africa and even Uganda itself. The Eastern Nilotic group also carries signals characteristic of West-Central Africa, primarily due to Bantu influence, whereas a much stronger signal in the Western Nilotic group suggests direct West-Central African ancestry. Bantu speakers share lineages with both Nilotic groups, and also harbour East African lineages not found in Western Nilotic speakers, likely due to assimilating indigenous populations since arriving in the region ~3000 years ago.