14 resultados para Miettinen, Timo: Pyhän merkkejä kivessä

em Universidade do Minho


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A urbanização de Vila d’Este é uma das mais importantes urbanizações de “habitação de custos controlados” em Portugal, com um total de 109 edifícios, alojando cerca de 17 mil habitantes. Tendo por base o processo de reabilitação em curso, é realizada uma análise de custos de ciclo de vida. O objetivo é identificar os níveis ótimos de rentabilidade dos requisitos energéticos aplicáveis na reabilitação dos edifícios e em cada um dos seus componentes com relevância no desempenho energético, bem como identificar os níveis de desempenho energético compatíveis com o conceito de edifícios de necessidades quase nulas de energia. Os resultados desta avaliação serão de utilidade relevante para o desenvolvimento do plano nacional para a reabilitação do parque edificado existente como previsto na Diretiva Europeia para o Desempenho Energético dos Edifícios de 2010.

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Poly(vinylidene fluoride) (PVDF) is a biocompatible material with excellent electroactive properties. Non-electroactive α-PVDF and electroactive β-PVDF were used to investigate the substrate polarization and polarity influence on the focal adhesion size and number as well as on human adipose stem cells (hASCs) differentiation. hASCs were cultured on different PVDF surfaces adsorbed with fibronectin and focal adhesion size and number, total adhesion area, cell size, cell aspect ratio and focal adhesion density were estimated using cells expressing EGFP-vinculin. Osteogenic differentiation was also determined using a quantitative alkaline phosphatase assay. The surface charge of the poled PVDF films (positive or negative) influenced the hydrophobicity of the samples, leading to variations in the conformation of adsorbed extracellular matrix (ECM) proteins, which ultimately modulated the stem cell adhesion on the films and induced their osteogenic differentiation.

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This work reports on the influence of the substrate polarization of electroactive β-PVDF on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and “poled -”) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. “Poled -” β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.

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Dissertação de mestrado em Economia Monetária, Bancária e Financeira

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Dissertação de mestrado Engenharia e Gestão da Qualidade

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Dissertação de mestrado integrado em Engenharia Biomédica (área de especialização em Eletrónica Médica)

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In this article, we develop a specification technique for building multiplicative time-varying GARCH models of Amado and Teräsvirta (2008, 2013). The variance is decomposed into an unconditional and a conditional component such that the unconditional variance component is allowed to evolve smoothly over time. This nonstationary component is defined as a linear combination of logistic transition functions with time as the transition variable. The appropriate number of transition functions is determined by a sequence of specification tests. For that purpose, a coherent modelling strategy based on statistical inference is presented. It is heavily dependent on Lagrange multiplier type misspecification tests. The tests are easily implemented as they are entirely based on auxiliary regressions. Finite-sample properties of the strategy and tests are examined by simulation. The modelling strategy is illustrated in practice with two real examples: an empirical application to daily exchange rate returns and another one to daily coffee futures returns.

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Dissertação de mestrado integrado em Engenharia Mecânica

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Doctoral Programme in Telecommunication - MAP-tele

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Projeto de investigação integrado de International Master in Sustainable Built Environment

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Dissertação de mestrado integrado em Engenharia Civil

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Tese de Doutoramento em Ciências da Saúde