Evidence for inter- and intra-species biofilm formation variability among a small group of coagulase-negative staphylococci

Coagulase-negative staphylococci (CoNS) are common bacterial colonisers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS species. Here, biofilms from six different CoNS species were characterised in terms of biofilm composition and architecture. Interestingly, the ability to form a thick biofilm was not associated with any particular species, and high variability on biofilm accumulation was found within the same species. Cell viability assays also revealed different proportions of live and dead cells within biofilms formed by different species, although this parameter was particularly similar at the intra-species level. On the other hand, biofilm disruption assays demonstrated important inter- and intra-species differences regarding extracellular matrix composition. Lastly, confocal laser scanning microscopy (CLSM) experiments confirmed this variability, highlighting important differences and common features of CoNS biofilms. We hypothesised that the biofilm formation heterogeneity observed was rather associated with biofilm matrix composition than with cells themselves. Additionally, our results indicate that polysaccharides, DNA and proteins are fundamental pieces in the process of CoNS biofilm formation.

Intra and inter-individual differences in social intelligence of Portuguese students

Social intelligence is a favorable condition for career decision-making and development. The social intelligence indices of Portuguese students in school years prior to a career transition are characterized and intra and interindividual differences are analyzed. Participants were 1095 students (552, 50.4% women) with a mean age of 14.78 years (SD = 1.86), in the 8th (542, 49.5%), 10th (295, 26.9%) and 11th (258, 23.6%) grades. The Cognitive Test of Social Intelligence (PCIS) was administered at two moments, six months apart. Results indicate that the 8th grade obtained higher average scores in Problem Solving, Motivation and Self-confidence (time 1), while the 10th grade obtained better results in Problem Solving, Motivation and Familiarity (time 2). Between the assessment moments, all school years register an increase in Problem Solving and Self-confidence in social situations. These results constitute favorable psychological conditions for the promotion of ethical questioning in career guidance interventions.

Challenges and confusion in media regulation: a four-country comparison

[Excerto] ln this chapter we discuss recent developments and challenges in European media and communication policy, focusing on the period following the 2008 global financial crisis. We are especially interested in the implications of the financial crisis and its political repercussions nationally (austerity measures and cuts to public services, growing anti-politics sentiments and widespread dissatisfaction with free-market capitalism and representative democracy) for media and communication policy, understood here in a broad sense, so as to include ali electronic communications, such as the Internet, mobile communications, social media etc. Our overarching concern is with the implications of developments in media and communication policy for the democratic functions of the media in Europe (...).

O papel da diplomacia económica na dinamização da economia: o caso de Cabo Verde

Dissertação de mestrado em Negócios Internacionais

Global validation of the WSES Sepsis Severity Score for patients with complicated intra-abdominal infections: a prospective multicentre study (WISS Study).

BACKGROUND To validate a new practical Sepsis Severity Score for patients with complicated intra-abdominal infections (cIAIs) including the clinical conditions at the admission (severe sepsis/septic shock), the origin of the cIAIs, the delay in source control, the setting of acquisition and any risk factors such as age and immunosuppression. METHODS The WISS study (WSES cIAIs Score Study) is a multicenter observational study underwent in 132 medical institutions worldwide during a four-month study period (October 2014-February 2015). Four thousand five hundred thirty-three patients with a mean age of 51.2 years (range 18-99) were enrolled in the WISS study. RESULTS Univariate analysis has shown that all factors that were previously included in the WSES Sepsis Severity Score were highly statistically significant between those who died and those who survived (p < 0.0001). The multivariate logistic regression model was highly significant (p < 0.0001, R2 = 0.54) and showed that all these factors were independent in predicting mortality of sepsis. Receiver Operator Curve has shown that the WSES Severity Sepsis Score had an excellent prediction for mortality. A score above 5.5 was the best predictor of mortality having a sensitivity of 89.2 %, a specificity of 83.5 % and a positive likelihood ratio of 5.4. CONCLUSIONS WSES Sepsis Severity Score for patients with complicated Intra-abdominal infections can be used on global level. It has shown high sensitivity, specificity, and likelihood ratio that may help us in making clinical decisions.

Emigrantes portugueses low cost: as perspetivas de ontem e de hoje

Dissertação de mestrado em Sociologia (área de especialização em Organizações e Trabalho)

Imagem de destinos turísticos: um estudo sobre as publicações brasileiras

Os estudos referentes ao turismo dedicam-se à compreensão de diferentes temas, sendo que um bastante analisado à escala internacional é a questão da imagem de um destino turístico. O interesse por este tema é percebido em diversas áreas do conhecimento, como a sociologia e o marketing. No Brasil, há diversos pesquisadores dedicando-se à imagem dos destinos turísticos. Assim, buscando compreender de que forma este tema tem sido trabalhado no Brasil, este estudo de caráter bibliográfico teve como objetivo analisar e compreender as publicações sobre imagem de destinos turísticos em periódicos brasileiros, nos últimos seis anos (2008-2014). A partir de um levantamento dos artigos publicados neste período, usando como ferramenta de busca o site “Publicações de Turismo”, foram identificados vinte e oito artigos. A análise permitiu verificar que os estudos estão concentrados na compreensão da imagem de um determinado destino. Os artigos analisados são predominantemente estudos empíricos e há um equilíbrio entre os de caráter quantitativo e qualitativo. No que diz respeito à bibliografia, foi possível identificar que os autores brasileiros que têm trabalhado com o tema de imagem de destinos, já buscam bibliografias internacionais para fundamentar os seus estudos. Também nos trabalhos sobre este tema, identificou-se uma maior maturidade dos autores no que diz respeito à utilização das ferramentas estatísticas. Alguns estudos fazem uma análise aprofundada dos estudos internacionais e aplicam estas teorias no Brasil. Conclui-se que este tema tem sido estudado com maior profundidade no país, se comparado com os temas como o da percepção de residentes.