Release of insulin from PLGA-alginate dressing stimulates regenerative healing of burn wounds in rats

Burn wound healing involves a complex set of overlapping processes in an environment conducive to ischemia, inflammation, and infection costing $7.5 billion/year in the US alone, in addition to the morbidity and mortality that occur when the burns are extensive. We previously showed that insulin, when topically applied to skin excision wounds, accelerates re-epithelialization, and stimulates angiogenesis. More recently, we developed an alginate sponge dressing (ASD) containing insulin encapsulated in PLGA microparticles that provides a sustained release of bioactive insulin for >20days in a moist and protective environment. We hypothesized that insulin-containing ASD accelerates burn healing and stimulates a more regenerative, less scarring, healing. Using a heat-induced burn injury in rats, we show that burns treated with dressings containing 0.04mg insulin/cm2, every three days for 9 days, have faster closure, faster rate of disintegration of dead tissue, and decreased oxidative stress.In addition, in insulin-treated wounds the pattern of neutrophil inflammatory response suggests faster clearing of the burn dead tissue. We also observe faster resolution of the pro-inflammatory macrophages. We also found that insulin stimulates collagen deposition and maturation with the fibers organized more like a basket weave (normal skin) than aligned and crosslinked (scar tissue). In summary , application of ASD-containing insulin-loaded PLGA particles on burns every three days stimulates faster and more regenerative healing. These results suggest insulin as a potential therapeutic agent in burn healing and, because of its long history of safe use in humans, insulin could become one of the treatments of choice when repair and regeneration are critical for proper tissue function.

Transições e percursos de alunos distinguidos na escola pública

A implementação de quadros de valor, mérito e excelência nas escolas portuguesas, nos quais se distinguem os alunos com comportamentos e desempenhos académicos exemplares, constitui o ponto de partida desta comunicação. Num primeiro momento, pretendemos abordar o processo de transição dos alunos distinguidos para o ensino superior. No momento seguinte, incidiremos o nosso olhar sobre as disposições destes jovens face ao ofício de aluno, aos atores e às instituições que marcaram os seus percursos e às expectativas que nortearam os diversos investimentos académicos. Os dados empíricos foram recolhidos no âmbito de um estudo de caso numa escola secundária do Norte de Portugal. Em relação aos alunos já ingressados no ensino superior e para aqueles que já concluíram este nível de ensino, tentaremos refletir sobre o grau de ajustamento destas experiências às expectativas inicialmente formuladas e/ou subsumidas nos sentidos das respostas e nas opções declaradas face ao futuro. Dos dados já obtidos, concluímos que a distinção académica parece escapar às lógicas do destino social, sendo a excelência escolar também visível em jovens de classes sociais mais baixas; concluímos também que estes percursos de excelência não têm na atualidade uma recompensa efetiva no acesso ao mercado de trabalho; e dentro do foco principal desta comunicação denotamos ainda a existência de transições não-lineares entre o secundário e o superior, designadamente no acesso ao curso pretendido e ao desempenho académico neste nível de ensino.

ACL injuries identifiable for pre-participation imagiological analysis: Risk factors

Identification of pre-participation risk factors for noncontact anterior cruciate ligament (ACL) injuries has been attracting a great deal of interest in the sports medicine and traumatology communities. Appropriate methods that enable predicting which patients could benefit from pre- ventive strategies are most welcome. This would enable athlete-specific training and conditioning or tailored equipment in order to develop appropriate strategies to reduce incidence of injury. In order to accomplish these goals, the ideal system should be able to assess both anatomic and functional features. Complementarily, the screening method must be cost-effective and suited for widespread application. Anatomic study protocol requiring only standard X rays could answer some of such demands. Dynamic MRI/CT evaluation and electronically assisted pivot-shift evaluation can be powerful tools providing complementary information. These upcoming insights, when validated and properly combined, envision changing pre-participation knee examination in the near future. Herein different methods (validated or under research) aiming to improve the capacity to identify persons/athletes with higher risk for ACL injury are overviewed. 

A prevenção de Lesões por Esforço Repetitivo (LER) nas aulas de saxofone

Relatório de estágio de mestrado em Ensino de Música