1 resultado para ageing workforce
em Institutional Repository of Leibniz University Hannover
Filtro por publicador
- ABACUS. Repositorio de Producción Científica - Universidad Europea (2)
- Aberdeen University (3)
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (2)
- Academic Research Repository at Institute of Developing Economies (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (5)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (3)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (16)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (1)
- Aston University Research Archive (46)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (8)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (11)
- Brunel University (1)
- Cambridge University Engineering Department Publications Database (30)
- CentAUR: Central Archive University of Reading - UK (41)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (5)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (45)
- CORA - Cork Open Research Archive - University College Cork - Ireland (5)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (150)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons - Montana Tech (1)
- Digital Commons @ DU | University of Denver Research (3)
- Digital Commons @ Winthrop University (1)
- Digital Commons at Florida International University (11)
- DigitalCommons@The Texas Medical Center (3)
- Diposit Digital de la UB - Universidade de Barcelona (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (2)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (6)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (7)
- Helda - Digital Repository of University of Helsinki (2)
- Indian Institute of Science - Bangalore - Índia (14)
- Institutional Repository of Leibniz University Hannover (1)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Politécnico do Porto, Portugal (1)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Iowa Publications Online (IPO) - State Library, State of Iowa (Iowa), United States (7)
- Massachusetts Institute of Technology (2)
- Ministerio de Cultura, Spain (2)
- National Center for Biotechnology Information - NCBI (1)
- Nottingham eTheses (4)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- Portal do Conhecimento - Ministerio do Ensino Superior Ciencia e Inovacao, Cape Verde (1)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (107)
- Queensland University of Technology - ePrints Archive (186)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Científico da Universidade de Évora - Portugal (4)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (18)
- Royal College of Art Research Repository - Uninet Kingdom (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- Scielo España (1)
- South Carolina State Documents Depository (1)
- Universidad de Alicante (2)
- Universidad Politécnica de Madrid (2)
- Universidade Técnica de Lisboa (1)
- Université de Lausanne, Switzerland (5)
- Université de Montréal, Canada (3)
- University of Canberra Research Repository - Australia (2)
- University of Connecticut - USA (2)
- University of Michigan (45)
- University of Queensland eSpace - Australia (28)
- University of Washington (6)
- WestminsterResearch - UK (5)
- Worcester Research and Publications - Worcester Research and Publications - UK (2)
Resumo:
Understanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike. Yet the potential for regulation at the transcriptional level has not been fully investigated. Here, we studied the relationship between seed viability, gene expression and glutathione redox status during artificial ageing of pea (Pisum sativum) seeds. Transcriptome-wide analysis using microarrays was complemented with qRT-PCR analysis of selected genes and a multilevel analysis of the antioxidant glutathione. Partial degradation of DNA and RNA occurred from the onset of artificial ageing at 60% RH and 50 degrees C, and transcriptome profiling showed that the expression of genes associated with programmed cell death, oxidative stress and protein ubiquitination were altered prior to any sign of viability loss. After 25 days of ageing viability started to decline in conjunction with progressively oxidising cellular conditions, as indicated by a shift of the glutathione redox state towards more positive values (>-190 mV). The unravelling of the molecular basis of seed ageing revealed that transcriptome reprogramming is a key component of the ageing process, which influences the progression of programmed cell death and decline in antioxidant capacity that ultimately lead to seed viability loss.