1 resultado para Near Field Communication
em Institutional Repository of Leibniz University Hannover
Filtro por publicador
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (1)
- Academic Research Repository at Institute of Developing Economies (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (9)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (6)
- Aquatic Commons (8)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (11)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (2)
- Archive of European Integration (23)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (1)
- Aston University Research Archive (16)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (8)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (11)
- Biodiversity Heritage Library, United States (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (32)
- Brock University, Canada (2)
- Bucknell University Digital Commons - Pensilvania - USA (2)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (13)
- Cambridge University Engineering Department Publications Database (76)
- CentAUR: Central Archive University of Reading - UK (53)
- Center for Jewish History Digital Collections (3)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (118)
- Cochin University of Science & Technology (CUSAT), India (11)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (2)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- Dalarna University College Electronic Archive (5)
- Deakin Research Online - Australia (24)
- Digital Commons - Michigan Tech (7)
- Digital Commons - Montana Tech (2)
- Digital Commons @ DU | University of Denver Research (2)
- Digital Commons at Florida International University (5)
- Digital Peer Publishing (1)
- DigitalCommons@The Texas Medical Center (4)
- Diposit Digital de la UB - Universidade de Barcelona (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (3)
- DRUM (Digital Repository at the University of Maryland) (4)
- Duke University (5)
- Earth Simulator Research Results Repository (1)
- Ecology and Society (2)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (7)
- Greenwich Academic Literature Archive - UK (3)
- Helda - Digital Repository of University of Helsinki (4)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (112)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Politécnico do Porto, Portugal (1)
- National Center for Biotechnology Information - NCBI (6)
- Nottingham eTheses (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (4)
- Portal de Revistas Científicas Complutenses - Espanha (6)
- Publishing Network for Geoscientific & Environmental Data (42)
- QSpace: Queen's University - Canada (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (84)
- Queensland University of Technology - ePrints Archive (64)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- RDBU - Repositório Digital da Biblioteca da Unisinos (3)
- Repositorio Académico de la Universidad Nacional de Costa Rica (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (4)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Digital da Universidade Municipal de São Caetano do Sul - USCS (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (26)
- Royal College of Art Research Repository - Uninet Kingdom (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (4)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Scielo España (1)
- Universidad de Alicante (4)
- Universidad Politécnica de Madrid (44)
- Universidade Complutense de Madrid (8)
- Universidade Federal do Pará (5)
- Universidade Federal do Rio Grande do Norte (UFRN) (1)
- Universita di Parma (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (1)
- Université de Montréal, Canada (5)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (9)
- University of Queensland eSpace - Australia (16)
- University of Washington (6)
- WestminsterResearch - UK (3)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Background: In molecular medicine, the manipulation of cells is prerequisite to evaluate genes as therapeutic targets or to transfect cells to develop cell therapeutic strategies. To achieve these purposes it is essential that given transfection techniques are capable of handling high cell numbers in reasonable time spans. To fulfill this demand, an alternative nanoparticle mediated laser transfection method is presented herein. The fs-laser excitation of cell-adhered gold nanoparticles evokes localized membrane permeabilization and enables an inflow of extracellular molecules into cells. Results: The parameters for an efficient and gentle cell manipulation are evaluated in detail. Efficiencies of 90% with a cell viability of 93% were achieved for siRNA transfection. The proof for a molecular medical approach is demonstrated by highly efficient knock down of the oncogene HMGA2 in a rapidly proliferating prostate carcinoma in vitro model using siRNA. Additionally, investigations concerning the initial perforation mechanism are conducted. Next to theoretical simulations, the laser induced effects are experimentally investigated by spectrometric and microscopic analysis. The results indicate that near field effects are the initial mechanism of membrane permeabilization. Conclusion: This methodical approach combined with an automated setup, allows a high throughput targeting of several 100,000 cells within seconds, providing an excellent tool for in vitro applications in molecular medicine. NIR fs lasers are characterized by specific advantages when compared to lasers employing longer (ps/ns) pulses in the visible regime. The NIR fs pulses generate low thermal impact while allowing high penetration depths into tissue. Therefore fs lasers could be used for prospective in vivo applications.