Villapaidoistako elämänlankaa? : Tutkimus neulonnasta harrastuksena

Tutkimuksen tavoitteena oli tarkastella neulonnan harrastamista: etsiä yhteyksiä perinteeseen, kartoittaa nykytilannetta ja hahmotella mahdollisia tulevaisuuden näkymiä. Tutkimuksen avulla haettiin vastausta neulontaharrastuksen viriämiseen liittyviin tekijöihin, harrastuksen ilmenemiseen ja neulonnan merkitykseen harrastajalle. Teoreettisen viitekehyksen mukaan neulontaa tarkasteltiin harrastuksena, käsityön osa-alueena jaopittuna taitona. Lisäksi neulonnan merkitystä harrastajalle lähestyttiin kartoittamalla merkitystentaustalla olevia arvoja ja asenteita. Tutkimuksen suoritustavaksi valittiin kyselylomakkeen avulla toteutettava kvantitatiivinen menetelmä, jota täydennettiin tulosten tulkinnassa käytetyllä kvalitatiivisella tutkimusotteella. Kyselylomakkeeseen sijoitettiin monivalintakysymysten ja väittämien lisäksi laajempi neulonnan merkitystä kartoittava avoin kysymys. Tavoitteena oli saada mahdollisimman laaja kuva neulonnan harrastamisesta Suomessa. Tutkimuksen yhteistyökumppaneiksi lupautuivat Helsingin villakehräämö ja Novita Neuleet -lehti, joiden avulla pystyttiin tavoittamaan neulonnan harrastajia ympäri Suomea. Tutkimus suoritettiin postikyselynä, joka lähetettiin Novita Neuleet -lehden tilaajille ja samansuuruiselle ryhmälle oletettuja neulonnan harrastajia. Otoksen koko oli 603 henkilöä, joista 325 palauttitäytetyn lomakkeen määräaikaan mennessä. Vastausten palautumisprosentti oli 54%. Aineiston analysoinnissa käytettiin SPSS-tilasto-ohjelmaa, jonka avulla aineistosta laadittiin frekvenssi- ja prosenttijakaumat sekä tarvittavat tunnusluvut. Ryhmien välisiä eroja testattiin ristiintaulukoinneilla ja tilastollisilla testeillä. Neulonnan arvostusta ja merkitystä kuvaavista väittämistä laadittiin faktorianalyysi mahdollisten neulojatyyppien muodostamista varten. Neulonnan merkitystä harrastajalle tulkittiin sisällön erittelyllä sekä olemustyyppianalyysiä soveltamalla. Tulosten perusteella neulontaharrastuksen viriämiseen liittyivät keskeisesti suvun ja perinteen merkitys, tarpeet ja koulukokemukset. Neulontaa harrastettiin paljon, sillä vastaajista 99% neuloi käsin vähintään yhden työn vuodessa. Eniten neulottiin villapaitoja (82%). Tilastollisesti Novita Neuleet -lehden tilaajat neuloivat vertailuryhmää enemmän. Neulonnan merkityksissä harrastajalle korostettiin hyödyllistä ajankäyttöä, rentoutumista, luovuutta ja itsensä ilmaisua. Neuleen valmiiksi saaminen tuotti mielihyvää, joka ilmeni antamisen ilona, haasteiden voittamisena ja onnistumisen kokemuksina. Harrastajien toimintaa kuvaamaan muodostettiin kaksi neulojatyyppiä: elämäntapa- ja tarveneulojat. Elämäntapaneulojilla on aina jokin neuletyö meneillään, kun taas tarveneulojat ryhtyvät neulomaan tarvitessaan esimerkiksi neulevaatteen tai käsilleen tekemistä. Villapaidoista voi saada elämänlankaa, sillä neulonnan todettiin tuovan harrastajan elämään sisältöä. Neulontaa pidettiin tulevaisuudessakin tarvittavana käsityötaitona, jonka haluttiin säilyvän tuleville sukupolville.

Molecular epidemiology of human rhinoviruses

The first part of this work investigates the molecular epidemiology of a human enterovirus (HEV), echovirus 30 (E-30). This project is part of a series of studies performed in our research team analyzing the molecular epidemiology of HEV-B viruses. A total of 129 virus strains had been isolated in different parts of Europe. The sequence analysis was performed in three different genomic regions: 420 nucleotides (nt) in the VP4/VP2 capsid protein coding region, the entire VP1 capsid protein coding gene of 876 nt, and 150 nt in the VP1/2A junction region. The analysis revealed a succession of dominant sublineages within a major genotype. The temporally earlier genotypes had been replaced by a genetically homogenous lineage that has been circulating in Europe since the late 1970s. The same genotype was found by other research groups in North America and Australia. Globally, other cocirculating genetic lineages also exist. The prevalence of a dominant genotype makes E-30 different from other previously studied HEVs, such as polioviruses and coxsackieviruses B4 and B5, for which several coexisting genetic lineages have been reported. The second part of this work deals with molecular epidemiology of human rhinoviruses (HRVs). A total of 61 field isolates were studied in the 420-nt stretch in the capsid coding region of VP4/VP2. The isolates were collected from children under two years of age in Tampere, Finland. Sequences from the clinical isolates clustered in the two previously known phylogenetic clades. Seasonal clustering was found. Also, several distinct serotype-like clusters were found to co-circulate during the same epidemic season. Reappearance of a cluster after disappearing for a season was observed. The molecular epidemiology of the analyzed strains turned out to be complex, and we decided to continue our studies of HRV. Only five previously published complete genome sequences of HRV prototype strains were available for analysis. Therefore, all designated HRV prototype strains (n=102) were sequenced in the VP4/VP2 region, and the possibility of genetic typing of HRV was evaluated. Seventy-six of the 102 prototype strains clustered in HRV genetic group A (HRV-A) and 25 in group B (HRV-B). Serotype 87 clustered separately from other HRVs with HEV species D. The field strains of HRV represented as many as 19 different genotypes, as judged with an approximate demarcation of a 20% nt difference in the VP4/VP2 region. The interserotypic differences of HRV were generally similar to those reported between different HEV serotypes (i.e. about 20%), but smaller differences, less than 10%, were also observed. Because some HRV serotypes are genetically so closely related, we suggest that the genetic typing be performed using the criterion "the closest prototype strain". This study is the first systematic genetic characterization of all known HRV prototype strains, providing a further taxonomic proposal for classification of HRV. We proposed to divide the genus Human rhinoviruses into HRV-A and HRV-B. The final part of the work comprises a phylogenetic analysis of a subset (48) of HRV prototype strains and field isolates (12) in the nonstructural part of the genome coding for the RNA-dependent RNA polymerase (3D). The proposed division of the HRV strains in the species HRV-A and HRV-B was also supported by 3D region. HRV-B clustered closer to HEV species B, C, and also to polioviruses than to HRV-A. Intraspecies variation within both HRV-A and HRV-B was greater in the 3D coding region than in the VP4/VP2 coding region, in contrast to HEV. Moreover, the diversity of HRV in 3D exceeded that of HEV. One group of HRV-A, designated HRV-A', formed a separate cluster outside other HRV-A in the 3D region. It formed a cluster also in the capsid region, but located within HRV-A. This may reflect a different evolutionary history of distinct genomic regions among HRV-A. Furthermore, the tree topology within HRV-A in the 3D region differed from that in the VP4/VP2, suggesting possible recombination events in the evolution of the strains. No conflicting phylogenies were observed in any of the 12 field isolates. Possible recombination was further studied using the Similarity and Bootscanning analyses of the complete genome sequences of HRV available in public databases. Evidence for recombination among HRV-A was found, as HRV2 and HRV39 showed higher similarity in the nonstructural part of the genome. Whether HRV2 and HRV39 strains - and perhaps also some other HRV-A strains not yet completely sequenced - are recombinants remains to be determined.

The Open Access Scientific Journal: An Empirical Study

The open access (OA) model for journals is compared to the open source principle for computer software. Since the early 1990s nearly 1,000 OA scientific journals have emerged – mostly as voluntary community efforts, although recently some professionally operating publishers have used author charges or institutional membership. This study of OA journals without author charges shows that their impact is still relatively small, but awareness of it is increasing. The average number of research articles per year is lower than for major scientific journals but the publication times are shorter.