Campylobacter Infections of Domestic Origin in Finland

Campylobacter jejuni and C. coli are the leading causes of human bacterial gastroenteritis in developed countries. Most human Campylobacter infections are sporadic and a seasonal peak in the distribution of infections can be seen in the summer months in several countries, including Finland. A variety of risk factors for Campylobacter infections have been identified; handling and eating poultry, drinking unpasteurized milk, contact with domestic animals, and travelling abroad. However, the relative importance of the different risk factors in sporadic cases of Campylobacter infection remains unknown. In most cases, the infection is self-limiting and no specific treatment is required. Campylobacter enteritis can cause a wide range of complications, including reactive arthritis (ReA) that is reported in 1-5% of the cases. Seven clinical microbiology laboratories serving different geographical areas of Finland, participated in this multi-centre study, conducted during a seasonal peak in 2002. In a matched case-control study, domestically-acquired sporadic Campylobacter infections from three geographical areas were collected. The final study comprised 100 cases and 137 controls. Risk factors for sporadic domestically-acquired Campylobacter infections were identified on the basis of a questionnaire; swimming in natural waters was found to be a novel risk factor for Campylobacter infection. Other independent risk factors were tasting or eating raw or undercooked meat and drinking untreated water from a dug well. The role of bacterial strain and host characteristics are not fully understood in Campylobacter infections. Exposure factors, demographical characteristics, and the serotype of the Campylobacter isolate may affect the severity of the enteritis. This cross-sectional study comprised 114 patients with C. jejuni enteritis, diagnosed in three clinical microbiology laboratories; most of the patients had participated in the previous case-control study. Swimming was associated with age ≤ 5 years and serotype Pen 6,7 was found significantly more often among patients reporting swimming. The geographical distribution among serotypes varied; serotype Pen 4-complex appeared more often in patients from urban areas and serotype Pen 21 among patients from more rural areas. Thus, risk factors and sources of infection for C. jejuni infection may vary among individuals depending on age and geographical location. The in vitro susceptibilities of C. jejuni and C. coli strains isolated from patients infected abroad (85 strains) or domestically (393 strains) revealed that susceptibility to erythromycin is still high, even among isolates of foreign origin. However, the novel antimicrobial agent telithromycin did not offer any advantage over erythromycin; isolates with high minimal inhibitory concentrations (MICs) for erythromycin also showed reduced susceptibility to telithromycin. Reduced susceptibility to fluoroquinolones was detected almost exclusively among isolates of foreign origin and half of these isolates with high MICs for fluoroquinolones also showed elevated MICs for doxycycline. Questionnaires concerning complications associated with C. jejuni enteritis were sent to patients two months after becoming ill; 201 patients from seven different geographical areas were included in the study. Musculoskeletal complications after C. jejuni infection were commonly reported by patients (39%). The incidence of classical ReA was 4% and that of Achilles enthesopathy and/or heel pain 9%. Other C. jejuni-associated reactive joint symptoms were commonly reported, however, due to their milder nature seldom seen and diagnosed by a physician. The severity of the enteritis may predict further complications; stomach ache during enteritis was connected to the development of later joint pain. Early antimicrobial treatment, within two days from the start of symptoms, shortened the duration of diarrhoea by two days but did not prevent later musculoskeletal complications. Campylobacter is an important human enteropathogen and causes a significant burden of illness. As the incidence of Campylobacter infections is high, the importance of the infection and the occurrence of complications will increase. This stresses the importance of understanding the risk factors for acquiring Campylobacter infection and how bacterial strain and host characteristics may affect the risk for infection. The role of antimicrobial treatment for acute Campylobacter enteritis seems to be marginal and should be used restrictively.

Genomic and functional profiling of gastric cancer

Helicobacter pylori infection is a risk factor for gastric cancer, which is a major health issue worldwide. Gastric cancer has a poor prognosis due to the unnoticeable progression of the disease and surgery is the only available treatment in gastric cancer. Therefore, gastric cancer patients would greatly benefit from identifying biomarker genes that would improve diagnostic and prognostic prediction and provide targets for molecular therapies. DNA copy number amplifications are the hallmarks of cancers in various anatomical locations. Mechanisms of amplification predict that DNA double-strand breaks occur at the margins of the amplified region. The first objective of this thesis was to identify the genes that were differentially expressed in H. pylori infection as well as the transcription factors and signal transduction pathways that were associated with the gene expression changes. The second objective was to identify putative biomarker genes in gastric cancer with correlated expression and copy number, and the last objective was to characterize cancers based on DNA copy number amplifications. DNA microarrays, an in vitro model and real-time polymerase chain reaction were used to measure gene expression changes in H. pylori infected AGS cells. In order to identify the transcription factors and signal transduction pathways that were activated after H. pylori infection, gene expression profiling data from the H. pylori experiments and a bioinformatics approach accompanied by experimental validation were used. Genome-wide expression and copy number microarray analysis of clinical gastric cancer samples and immunohistochemistry on tissue microarray were used to identify putative gastric cancer genes. Data mining and machine learning techniques were applied to study amplifications in a cross-section of cancers. FOS and various stress response genes were regulated by H. pylori infection. H. pylori regulated genes were enriched in the chromosomal regions that are frequently changed in gastric cancer, suggesting that molecular pathways of gastric cancer and premalignant H. pylori infection that induces gastritis are interconnected. 16 transcription factors were identified as being associated with H. pylori infection induced changes in gene expression. NF-κB transcription factor and p50 and p65 subunits were verified using elecrophoretic mobility shift assays. ERBB2 and other genes located in 17q12- q21 were found to be up-regulated in association with copy number amplification in gastric cancer. Cancers with similar cell type and origin clustered together based on the genomic localization of the amplifications. Cancer genes and large genes were co-localized with amplified regions and fragile sites, telomeres, centromeres and light chromosome bands were enriched at the amplification boundaries. H. pylori activated transcription factors and signal transduction pathways function in cellular mechanisms that might be capable of promoting carcinogenesis of the stomach. Intestinal and diffuse type gastric cancers showed distinct molecular genetic profiles. Integration of gene expression and copy number microarray data allowed the identification of genes that might be involved in gastric carcinogenesis and have clinical relevance. Gene amplifications were demonstrated to be non-random genomic instabilities. Cell lineage, properties of precursor stem cells, tissue microenvironment and genomic map localization of specific oncogenes define the site specificity of DNA amplifications, whereas labile genomic features define the structures of amplicons. These conclusions suggest that the definition of genomic changes in cancer is based on the interplay between the cancer cell and the tumor microenvironment.

Global capitalism meets national spirit: Discourses in media texts on a cross-border acquisition

In this article, the authors explore media coverage of a recent acquisition across national borders. Their starting point is that the media represent a key arena of “discursive strategizing” for actors such as corporate managers. They illustrate and specify how global capitalism, as discourse relying on economic and financial rationale and exemplified here by the acquiring firm’s attempts to expand, meets national spirit, exemplified here by the complexity in selling the acquisition target to foreigners. The main contribution of this study lies in identifying how key actors draw on and mobilize rationalistic and nationalistic discourses in public discussion. The analysis illustrates that the same actors can draw on different—even contradictory—discourses at different points in time. Furthermore, different actors—even with opposing objectives—may draw on the same discourse in legitimizing their positions and pursuing specific ends.

Language and the Circuits of Power in a Merging Multinational Corporation

We argue in this paper that corporate language policies have significant power implications that are easily overlooked. By drawing on previous work on power in organizations (Clegg, 1989), we examine the complex power implications of language policy decisions by looking at three levels of analysis: episodic social interaction, identity/subjectivity construction, and reconstruction of structures of domination. In our empirical analysis, we focus on the power implications of the choice of Swedish as the corporate language in the case of the recent banking sector merger between the Finnish Merita and the Swedish Nordbanken. Our findings show how language skills become empowering or disempowering resources in organizational communication, how these skills are associated with professional competence, and how this leads to the creation of new social networks. The case also illustrates how language skills are an essential element in the construction of international confrontation, lead to a construction of superiority and inferiority, and also reproduce post-colonial identities in the merging bank. Finally, we also point out how such policies ultimately lead to the reification of post-colonial and neo-colonial structures of domination in multinational corporations.

Offering Development in the Restaurant Sector. A Comparison Between Customer Perceptions and Management Beliefs

The present study concentrates on a small – but important – area of marketing: offering development within the service sector, more exactly the restaurant sector. The empirical part of the study has been carried out in the Helsinki metropolitan area using six successful restaurants. First, a conceptual offering development model is developed based on how the management perceives the offering development processes. Second, customer perceptions of offerings and management beliefs about how the customers perceive the offerings are analysed. Finally, an extended offering development model is created based on the management perceptions (the first model) as well as on observed gaps between customer perceptions of offerings and management beliefs about the customer perceptions. The study reveals that customer perceptions and management beliefs are rather similar but also that some differences exist. These differences are taken into account in the extended offering development model (the second model). The empirical data was collected through interviews and surveys. All together 393 customers and 14 managers participated in the study. The study suggests that successful offering development has to be closely connected with the general strategy of the company. A shared vision within the company in combination with a systematic strategic offering development process create a sound basis for the practical development work. The main contribution of the study is the extended offering development model forming a framework for further studies within the area.

Museoesineitä Itä-Karjalasta : Väinö Tuomaalan jatkosodan aikana keräämä esinekokoelma ja sen syntyhistoria

Jatkosodan aikana kerättiin Suomen valtaamalta alueelta Itä-Karjalasta tuhansittain museoesineitä Suomen museoihin. Kansallismuseon kokoelmiin kerättiin esineitä lähinnä suomenheimoisilta mutta myös isovenäläisiltä. Tämän tutkimuksen kohteena on lähes 400:n esineen kokoelma, joka toimitettiin Kansallismuseoon vuosina 1941 1944 ja merkittiin kansallisuudeltaan venäläiseksi. Näistä venäläisistä esineistä suurimman osan keräsi alikersanttina palvellut Väinö Tuomaala. Tutkimukseni tarkastelee Väinö Tuomaalan kokoelmaa ja etsii syitä siihen, miksi kokoelma on sellainen kuin se on, miksi se ja koko venäläisten esineiden kokoelma syntyi ja mitkä seikat vaikuttivat Tuomaalan esinevalintoihin. Tutkimuksen teoria rakentuu museologisen kirjallisuuden varaan. Tutkimuksessa tarkastellaan Väinö Tuomaalan kokoelman keräyskonteksteja. Keräyskonteksteihin kuuluvat kokoelman kerääjä, keräysajankohta ja -paikka sekä yhteenkuuluvat esineet, eli muut venäläiset esineet. Kerääjän tarkastelussa huomioidaan hänen henkilöhistoriansa ja etsitään mahdollisia keruuseen vaikuttaneita tekijöitä. Keräysajankohdan tarkastelussa huomio kiinnittyy keruuympäristöön, eli vallitseviin olosuhteisiin ja aatteelliseen ilmapiiriin, joiden vaikutuksen alaisena keruu suoritettiin. Primääriaineisto koostuu Kansallismuseon alaisen Kulttuurien museon yleisetnografisten esineiden kokoelmaan kuuluvista 198:sta Väinö Tuomaalan keräämästä esineestä verifikaatteineen, jotka ovat osa 393 esinettä käsittävästä venäläisestä kokoelmasta. Aineisto sisältää myös eri arkistojen materiaalia. Kansallisarkiston Sörnäisten toimipisteestä, entisestä sota-arkistota, löytyneitä sotapäiväkirjoja ja Tuomaalan kantakorttia on tutkimuksessa käytetty jatkosodan tapahtumien esittämiseen. Museoviraston hallinto-osaston arkiston kirjeet ja pöytäkirjat ovat antaneet tietoa kulttuurikeruun organisoinnista. Väinö Tuomaalan arkistokokoelma Seinäjoella sisältää Tuomaalan keräämää perinneaineistoa, hänen puheitaan, lehtikirjoituksiaan ja kirjeenvaihtoaan. Myös Evijärven kunnantalon kotiseutuarkistosta löytyneet kirjeet ovat valottaneet Tuomaalan jatkosodan aikaista keruutoimintaa. Suomalaisen Kirjallisuuden Seuran arkistot ovat tarjonneet materiaalia jatkosodan aikaisesta kulttuurikeruusta. Koko venäläisten esineiden kokoelma syntyi sattumalta ja organisoimattoman toiminnan tuloksena, jossa aktiivisia toimijoita olivat yksittäiset kerääjät. Intohimoisesta suhtautumisesta kansatieteellistä keruuta kohtaan johtuen, Tuomaala olisi todenäköisesti kerännyt museoesineitä missä päin Itä-Karjalaa tahansa. Väinö Tuomaalan Itä-Karjalassa keräämä esinekokoelma oli luonnollinen jatke hänen kotiseudullaan aloittamalleen keräystoiminnalle. Tuomaalan jatkosodan aikana keräämä kokoelma on kerääjän mieltymysten mukainen yritys kerätä vanhaa itäkarjalaista, kansallista talonpoikaiskulttuuria. Tuomaala pyrki keräämään klassista kansatieteellistä aineistoa monipuolisesti kansankulttuurin eri elämänalueilta. Tuomaalan esinekeruussa näkyi vahvasti talonpoikaiskulttuurin ihannointi ja halu pelastaa osa siitä tuleville sukupolville. Yrityksen onnistumiseen vaikuttivat sodanajan olosuhteet sekä aatteellinen ilmapiiri. Esineiden saatavuudella oli merkittävä osa kokoelman muodostumiseen, samoin Tuomaalan käsityksillä heimokansoista, Suur-Suomesta ja venäläisistä. Keruuvalintoihin ja keruun jatkumiseen vaikuttivat myös Kansallismuseon henkilökunta ja työstä saatu tunnustus.

Private and municipal forests and the forestry planning system in Japan - trends and problems after World War II.

XVIII IUFRO World Congress, Ljubljana 1986.