Nitrogen use efficiency in summer sorghum grown on clay soils

Nitrogen fertilizer inputs dominate the fertilizer budget of grain sorghum growers in northern Australia, so optimizing use efficiency and minimizing losses are a primary agronomic objective. We report results from three experiments in southern Queensland sown on contrasting soil types and with contrasting rotation histories in the 2012-2013 summer season. Experiments were designed to quantify the response of grain sorghum to rates of N fertilizer applied as urea. Labelled 15N fertilizer was applied in microplots to determine the fate of applied N, while nitrous oxide (N2O) emissions were continuously monitored at Kingaroy (grass or legume ley histories) and Kingsthorpe (continuous grain cropping). Nitrous oxide is a useful indicator of gaseous N losses. Crops at all sites responded strongly to fertilizer N applications, with yields of unfertilized treatments ranging from 17% to 52% of N-unlimited potential. Maximum yields ranged from 4500 (Kupunn) to 5450 (Kingaroy) and 8010 (Kingsthorpe) kg/ha. Agronomic efficiency (kg additional grain produced/kg fertilizer N applied) at the optimum N rate on the Vertosol sites was 23 (80 N, Kupunn) to 25 (160N, Kingsthorpe), but 40-42 on the Ferrosols at Kingaroy (70-100N). Cumulative N2O emissions ranged from 0.44% (Kingaroy legume) to 0.93% (Kingsthorpe) and 1.15% (Kingaroy grass) of the optimum fertilizer N rate at each site, with greatest emissions from the Vertosol at Kingsthorpe. The similarity in N2O emissions factors between Kingaroy and Kingsthorpe contrasted markedly with the recovery of applied fertilizer N in plant and soil. Apparent losses of fertilizer N ranged from 0-5% (Ferrosols at Kingaroy) to 40-48% (Vertosols at Kupunn and Kingsthorpe). The greater losses on the Vertosols were attributed to denitrification losses and illustrate the greater risks of N losses in these soils in wet seasonal conditions.

What influences fungal communities in cotton soils

Soilborne diseases such as Fusarium wilt, Black root rot and Verticillium wilt have significant impact on cotton production. Fungi are an important component of soil biota with capacity to affect pathogen inoculum levels and their disease causing potential. Very little is known about the soil fungal community structure and management effects in Australian cotton soils. We analysed surface soils from ongoing field experiments monitoring cotton performance and disease incidence in three cotton growing regions, collected prior to 2013 planting, for the genetic diversity and abundance as influenced by soil type, environment and management practices and link it with disease incidence and suppression. Results from the 28S LSU rRNA sequencing based analysis indicated a total of 370 fungal genera in all the cotton soils and the top 25 genera in abundance accounted for the major portion of total fungal community. There were significant differences in the composition and genetic diversity of soil fungi between the different field sites from the three cotton growing regions. Results for diversity indices showed significantly greater diversity in the long-term crop rotation experiment at Narrabri (F6E) and experiments at Cowan and Goondiwindi compared to the Biofumigation and D1 field experiments at ACRI, Narrabri. Diversity was lowest in the soils under brassica crop rotation in Biofumigation experiment. Overall, the diversity and abundance of soil fungal community varied significantly in the three cotton growing regions indicating soil type and environmental effects. These results suggest that changes in soil fungal community may play a notable role in soilborne disease incidence in cotton.

Potential of Trichoderma harzianum for control of banana leaf fungal pathogens when applied with a food source and an organic adjuvant

Trichoderma isolates were obtained from diseased leaves and fruit collected from plantations in the main banana production area in Northern Queensland. Phylogenetic analyses identified the Trichoderma isolates as T. harzianum and T. virens. The Trichoderma spp. were found to be antagonistic against the banana leaf pathogens Mycosphaerella musicola, Cordana musae, and Deight-oniella torulosa in vitro. Several products used by the banana industry to increase production, including molasses, Fishoil and Seasol, were tested as food source for the Trichoderma isolates. The optimal food substrate was found to be molasses at a concentration of 5 %, which when used in combination with a di-1-p-menthene spreader-sticker enhanced the survivability of Trichoderma populations under natural conditions. This formulation suppressed D. torulosa development under glasshouse conditions. Furthermore, high sensitivity was observed towards the protectant fungicide Mancozeb but Biopest oil (R), a paraffinic oil, only marginally suppressed the growth of Trichoderma isolates in vitro. Thus, this protocol represents a potential to manage banana leaf pathogens as a part of an integrated disease approach.