Predicting survival of western rock lobsters Panulirus cygnus using discriminant analysis of hemolymph parameters taken immediately following simulated handling treatments

Instances of morbidity amongst rock lobsters (Panulirus cygnus) arriving at factories in Western Australia (WA) have been attributed to stress during post-harvest handling. This study used discriminant analysis to determine whether physiological correlates of stress following a period of simulated post-harvest handling had any validity as predictors of future rejection or morbidity of western rock lobsters. Groups of 230 western rock lobsters were stored for 6 h in five environments (submerged/flowing sea water, submerged/re-circulating sea water, humid air, flowing sea water spray, and re-circulated sea water spray). The experiment was conducted in late spring (ambient sea water 22°C), and repeated again in early autumn (ambient sea water 26°C). After 6 h treatment, each lobster was graded for acceptability for live export, numbered, and its hemolymph was sampled. The samples were analysed for a number of physiological and health status parameters. The lobsters were then stored for a week in tanks in the live lobster factory to record mortality. The mortality of lobsters in the factory was associated with earlier deviations in hemolymph parameters as they emerged from the storage treatments. Discriminant analysis (DA) of the hemolymph assays enabled the fate of 80-90% of the lobsters to be correctly categorised within each experiment. However, functions derived from one experiment were less accurate at predicting mortality when applied to the other experiments. One of the reasons for this was the higher mortality and the more severe patho-physiological changes observed in lobsters stored in humid air or sprays at the higher temperature. The analysis identified lactate accumulation during emersion and associated physiological and hemocyte-related effects as a major correlate of mortality. Reducing these deviations, for example by submerged transport, is expected to ensure high levels of survival. None of the indicators tested predicted mortality with total accuracy. The simplest and most accurate means of comparing emersed treatments was to count the mortality afterwards.

Reproductive biology of mangrove jack (Lutjanus argentimaculatus) in northeastern Queensland, Australia

Lutjanus argentimaculatus is an Indo-Pacific species that inhabits riverine, coastal and offshore reef habitats. An investigation of the reproductive biology of Lutjanus argentimaculatus in northeastern Queensland waters (Australia) was undertaken between 1999 and 2002. Individuals in inshore estuarine and freshwater riverine habitats were mostly immature whereas those captured in offshore reef waters were predominantly mature. Males matured at a smaller size than females, with the length-at-50%-maturity (Lm50) for males estimated to be 470.7 mm fork length (FL) and 531.4 mm FL for females. The spawning season in northeastern Queensland was mostly during the austral spring-summer and peaked in December. The presence of ripe female fish and occurrence of postovulatory follicles in histological sections provided evidence that spawning activity was more pronounced during the full and third quarter moon phases. Lutjanus argentimaculatus were highly fecund with estimates of up to 4 x 106 ova per spawning event. Immature fish concentrated in inshore areas where they were targeted by recreational fishers whereas, in offshore areas, commercial fishers caught predominantly larger, mature fish.

Two varieties of the invasive liana, cat’s claw creeper, Macfadyena unguis-cati (Bignoniaceae) in Queensland, Australia

The invasive liana cat’s claw creeper, Macfadyena unguis-cati, native to tropical Central and South America, is a major environmental weed in Queensland and New South Wales (NSW). Two morphologically distinct cat’s claw creeper varieties occur in Australia, a ‘short-pod’ variety that is widespread through Queensland and NSW and a ‘long-pod’ variety restricted to a few sites in southeast Queensland. In this study we report the differences in the above-ground morphological, phenological and reproductive traits between the two varieties. The ‘long-pod’ variety has significantly larger leaves, larger pods, and larger number of seeds per pod than the ‘short-pod’ variety. The ‘short-pod’ variety has a slightly wider pods, and thicker leaves than the ‘long-pod’ variety. Both varieties have a yellow trumpet shaped flower, but the flower of the ‘long-pod’ variety has a deeper hue of yellow than the ‘short-pod’ flower. The fruits of the ‘short-pod’ variety mature in late summer to early autumn while the fruits of ‘long-pod’ variety mature in late winter to early spring. The more widespread nature of the ‘short-pod’ variety could potentially be due to a preference for this variety as an ornamental plant, due to its more presentable foliage characteristics and shorter pods, in contrast to the ‘long-pod’ variety.