A checklist of plant pathogenic and other microfungi in the rainforests of the wet tropics of northern Queensland

Microfungi that cause disease or are associated with diseased plants in the wet tropics of northern Queensland are listed. A total of 206 host-pathogen combinations on 148 host species has been compiled from the results of plant disease surveys in the Wet Tropics World Heritage Area in 1992 and 1993, from herbarium records and from previously published host-pathogen combinations.

A one-tube fluorescent assay for the quarantine detection and identification of Tilletia indica and other grass bunts in wheat

A molecular assay with enhanced specificity and sensitivity has been developed to assist in the surveillance of Karnal bunt, a quarantineable disease with a significant impact on international trade. The protocol involves the release of DNA from spores, PCR amplification to enrich Tilletia-specific templates from released DNA and a five-plex, real-time PCR assay to detect, identify and distinguish T. indica and other Tilletia species (T. walkeri, T. ehrhartae, T. horrida and a group comprising T. caries, T. laevis, T. contraversa, T. bromi and T. fusca) in wheat grains. This fluorescent molecular tool has a detection sensitivity of one spore and thus bypasses the germination step, which in the current protocol is required for confirmation when only a few spores have been found in grain samples. The assay contains five dual-labelled, species-specific probes and associated species-specific primer pairs in a PCR mix in one tube. The different amplification products are detected simultaneously by five different fluorescence spectra. This specific and sensitive assay with reduced labour and reagent requirements makes it an effective and economically sustainable tool to be used in a Karnal bunt surveillance program. This protocol will also be valuable for the identification of some contaminant Tilletia sp. in wheat grains.

Genera of the leaf-feeding Dendrothripinae of the world (Thysanoptera, Thripidae), with new species from Australia and Sulawesi, Indonesia

Character states used in distinguishing taxa within the Thripidae subfamily Dendrothripinae are discussed, and a key presented to the 11 genera recognized worldwide. Comments on each of these genera are provided, together with keys to the species from Australia of Dendrothrips, Ensiferothrips and Pseudodendrothrips. From Australia are described, four new species of Dendrothrips, one of Pseudodendrothrips, and a remarkable new species of Ensiferothrips that has required a re-diagnosis of that genus. Another new species of Ensiferothrips is described from Sulawesi, thus greatly extending the known geographical range of this previously Australian genus. © 2016 Magnolia Press.