2 resultados para geographical location

em Universidade Complutense de Madrid


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For more than half of a century, Colombia has been living in a state of violence, a nationwide political violence. As the time goes by, this situation gets even worse. Now the violence is implanted for different interests, such as personal, political, social and economical interests. For this reason, the information for this thesis was gathered from the time of the independence, through the era of violence until today; considering a reflection that begins with the perspective of Marx, passing by a theoretical compilation of the conflict, with an anthropological, psychological, biological and sociological perspective. In addition, different theories about violence have been studied to recognize the ideological approach of the different armed movements that have emerged in Colombia. Statistical, economical and social data including class struggle, social stratification, exclusion and gender perspective among others, have been considered from an anthropological and interdisciplinary approach for studying the violence. A strong interest in the social reintegration process, through an ethnographic study, based on the actors backgrounds, their lives, experiences and their geographical location have provided information that will allow to know the reality of those who are living in the process, those who survive it and those who go on with their lives in society, and those who after doing it return to the armed groups. Population displaced by violence, refugees, has contributed to this study about their possibilities to return to the society or to be excluded by it. With this purpose, a theoretical and a documentary analysis as well as fieldwork have been done, trying to bring forward tools and guidelines to make some progress in developing effective solutions for social integration of Colombian armed conflict victims.

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BACKGROUND Mycobacterium avium subspecies paratuberculosis (Map) causes an infectious chronic enteritis (paratuberculosis or Johne's disease) principally of ruminants. The epidemiology of Map is poorly understood, particularly with respect to the role of wildlife reservoirs and the controversial issue of zoonotic potential (Crohn's disease). Genotypic discrimination of Map isolates is pivotal to descriptive epidemiology and resolving these issues. This study was undertaken to determine the genetic diversity of Map, enhance our understanding of the host range and distribution and assess the potential for interspecies transmission. RESULTS 164 Map isolates from seven European countries representing 19 different host species were genotyped by standardized IS900--restriction fragment length polymorphism (IS900-RFLP), pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphisms (AFLP) and mycobacterial interspersed repeat unit-variable number tandem repeat (MIRU-VNTR) analyses. Six PstI and 17 BstEII IS900-RFLP, 31 multiplex [SnaBI-SpeI] PFGE profiles and 23 MIRU-VNTR profiles were detected. AFLP gave insufficient discrimination of isolates for meaningful genetic analysis. Point estimates for Simpson's index of diversity calculated for the individual typing techniques were in the range of 0.636 to 0.664 but a combination of all three methods increased the discriminating power to 0.879, sufficient for investigating transmission dynamics. Two predominant strain types were detected across Europe with all three typing techniques. Evidence for interspecies transmission between wildlife and domestic ruminants on the same property was demonstrated in four cases, between wildlife species on the same property in two cases and between different species of domestic livestock on one property. CONCLUSION The results of this study showed that it is necessary to use multiple genotyping techniques targeting different sources of genetic variation to obtain the level of discrimination necessary to investigate transmission dynamics and trace the source of Map infections. Furthermore, the combination of genotyping techniques may depend on the geographical location of the population to be tested. Identical genotypes were obtained from Map isolated from different host species co-habiting on the same property strongly suggesting that interspecies transmission occurs. Interspecies transmission of Map between wildlife species and domestic livestock on the same property provides further evidence to support a role for wildlife reservoirs of infection.