Utility of a panviral microarray for detection of swine respiratory viruses in clinical samples

Several factors have recently converged, elevating the need for highly parallel diagnostic platforms that have the ability to detect many known, novel, and emerging pathogenic agents simultaneously. Panviral DNA microarrays represent the most robust approach for massively parallel viral surveillance and detection. The Virochip is a panviral DNA microarray that is capable of detecting all known viruses, as well as novel viruses related to known viral families, in a single assay and has been used to successfully identify known and novel viral agents in clinical human specimens. However, the usefulness and the sensitivity of the Virochip platform have not been tested on a set of clinical veterinary specimens with the high degree of genetic variance that is frequently observed with swine virus field isolates. In this report, we investigate the utility and sensitivity of the Virochip to positively detect swine viruses in both cell culture-derived samples and clinical swine samples. The Virochip successfully detected porcine reproductive and respiratory syndrome virus (PRRSV) in serum containing 6.10 × 10(2) viral copies per microliter and influenza A virus in lung lavage fluid containing 2.08 × 10(6) viral copies per microliter. The Virochip also successfully detected porcine circovirus type 2 (PCV2) in serum containing 2.50 × 10(8) viral copies per microliter and porcine respiratory coronavirus (PRCV) in turbinate tissue homogenate. Collectively, the data in this report demonstrate that the Virochip can successfully detect pathogenic viruses frequently found in swine in a variety of solid and liquid specimens, such as turbinate tissue homogenate and lung lavage fluid, as well as antemortem samples, such as serum.

Literatura y pensamiento en Francia. Un ejemplo : el panfleto

First of all, with this dissertation we have set as principal goal to define and to characterize the pamphlet in a French-speaking context. To do this, we will try to delimit this type of text and to demonstrate that in many occasions this term is used for classifying documents whose principal characteristic is the polemic. In the approach to this dissertation, we have mainly found two problems: on the one hand to define what a pamphlet is and to demarcate it from other similar texts both from the point of view of the content as well as the form; and on the other hand, to try to discern if the pamphlet can be included under the title of Literature and if it constitutes a literary genre for itself. To do so, we will structure our investigation in several chapters gathered in two clearly delimited parts. At the end of each of them, we will draw some brief conclusions. The first part will be focused on the study of theoretical notions. We will approach this reflection from different perspectives: etymological, comparative, literary and generic. To do this, we will approach the theories of those schools or of those authors who are more convenient to our purpose, so both our matter of study and the areas where we want to study it, raise many problems of interpretation and classification. Our aim is not to do a study on the Literature concepts or on the Literary Genre, so we will simplify those theories that are related to its main characteristics...