Historia de la dirección escénica en España: Miguel Narros

The year 2013 saw the passing of Miguel Narros, one of the most outstanding men of theatre of recent decades, and a creator to whom we remain in debt today. His extensive legacy deserves a special place in our memory and stands as a subject of study of the keenest interest, in view of the increasing amount of research being done on the discipline of stage direction. The objective of the thesis being presented is to provide an overview of Narros’ work, so as to draw conclusions related to the situation of theatre in Spain throughout the second half of the twentieth century and in the early twenty-first century and to elucidate the director’s poetic conception and a theory of his stage practice. The thesis has focused on the director’s biography —in which work and personal life are closely intertwined—, the artistic and technical credits and dates of his stage productions, the compiling and summarising of a number of reviews in the press, the classification and discussion of the different historical and literary periods dealt with by the director, as well as the poetics of his theatre (his points of reference, his conception as stage director —form and content— and his position on the elements that make up a stage production). Also attached is a selection of photographs of more than half of his stagings (in addition to some of the director himself) which are testimony to his creation and a reflection of a number of the characteristics of his theatre. The study —based on information from the written press, public and private archives (which provided everything from photographs to handbills) and a number of personal interviews, among other sources— reveals a professional whose work transformed, enriched and consolidated the Spanish stage. Indeed, Spanish theatre simply cannot be understood without taking into account Miguel Narros. Narros worked as an actor and immersed himself in the teachings of the figures that populated the theatre world of the mid-twentieth century, such as Jardiel Poncela, Elvira Noriega, José María Rodero, Carmen Seco and, above all, Luis Escobar...

Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids

In most gram-negative bacteria, acquired multiresistance is conferred by large plasmids compiling numerous antimicrobial resistance genes. Here, we show an evolutionary alternative strategy used by Pasteurella multocida to become resistant to multiple clinically relevant antibiotics. Thirteen beta-lactam-resistant clinical isolates, concomitantly resistant to tetracyclines and/or streptomycin as well as to sulfonamides, were studied. Pulsed-field gel electrophoresis analysis revealed different profiles among the isolates, showing that clonal dissemination was not the sole event responsible for the spread of multiresistance. Each P. multocida strain carried two or three small plasmids between 4 and 6 kb in size. A direct association between resistance profile and plasmid content was found. Complete nucleotide sequencing of all plasmids revealed seven different replicons, six of them belonging to the ColE1 superfamily. All plasmids carried one, or a maximum of two, antimicrobial resistance determinants. Plasmids pB1000 and pB1002 bore bla(ROB-1), pB1001 carried tet(B), pB1003 and pB1005 carried sul2 and strA, pB1006 harbored tet(O), and p9956 bore the tet(H) gene. All plasmids except pB1002 and pB1006 were successfully transformed into Escherichia coli. pB1000, also involved in beta-lactam resistance in Haemophilus parasuis (A. San Millan et al., Antimicrob. Agents Chemother. 51:2260-2264, 2007), was mobilized in E. coli using the conjugation machinery of an IncP plasmid. Stability experiments proved that pB1000 was stable in P. multocida but highly unstable in E. coli. In conclusion, bla(ROB-1) is responsible for beta-lactam resistance in P. multocida in Spain. Coexistence and the spread of small plasmids are used by P. multocida to become multiresistant.