2 resultados para Research application
em Universidade Complutense de Madrid
Resumo:
Poets' thoughts about poetry have long been the subject of much attention. Since Classical Antiquity this has given rise to powerful reflections about literature, influencing texts which would then become part of academic discourse. However, these have also been used to help to explain or exemplify questions the academic discipline of Literary Theory have failed to address properly, since such questions related to the process of creation justifiably give attention to the writers' own experiences of such a process. Nevertheless, although the content of texts in which poets explain their own creation or specify key aspects of the literary process has been taken into account, there has not been any systematic study of the formal aspects of the discourse coming from the creator. The reason lies in the fact that the discourse of the creator takes different and variant directions that complicate its definition and distinction in relation to other kinds of similar texts. The first part, theoretical in nature, has emerged from the study of explicit poetics by numerous authors, as well as from critical bibliography. This seeks to identify common characteristics of these texts. Conversely, the second and third parts aims to find the individuality of Colinas' poetics, which manifests itself fundamentally in its content. Because of this, it is the study of the core parts of Colinas' poetic thinking expressed in the trends of his explicit poetics that will constitute a substantial part of this section of research...
Resumo:
Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (Sus scrofa), to evaluate the use of several enzyme-linked immunosorbent assays (ELISAs) and lateral flow tests for in vivo TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-β), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in regions that are endemic for M. bovis and for TB vaccine research.