1 resultado para Project 2001-013-B : Sustainability and the Future Building Code of Australia
em Universidade Complutense de Madrid
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Repository Napier (3)
- Aberdeen University (4)
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (3)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (2)
- Aquatic Commons (16)
- Archive of European Integration (117)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (15)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (4)
- Biblioteca Digital de la Universidad Católica Argentina (1)
- Biodiversity Heritage Library, United States (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (43)
- Boston University Digital Common (2)
- Brock University, Canada (10)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (3)
- Cambridge University Engineering Department Publications Database (6)
- CentAUR: Central Archive University of Reading - UK (48)
- Center for Jewish History Digital Collections (8)
- Central European University - Research Support Scheme (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (14)
- Cochin University of Science & Technology (CUSAT), India (2)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (35)
- CORA - Cork Open Research Archive - University College Cork - Ireland (1)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- CUNY Academic Works (1)
- Deakin Research Online - Australia (93)
- Department of Computer Science E-Repository - King's College London, Strand, London (1)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Archives@Colby (3)
- Digital Commons - Michigan Tech (1)
- Digital Commons @ DU | University of Denver Research (2)
- Digital Commons @ Winthrop University (1)
- Digital Commons at Florida International University (14)
- Digital Peer Publishing (1)
- DigitalCommons@The Texas Medical Center (2)
- DigitalCommons@University of Nebraska - Lincoln (3)
- Digitale Sammlungen - Goethe-Universität Frankfurt am Main (1)
- Duke University (2)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (5)
- Glasgow Theses Service (2)
- Greenwich Academic Literature Archive - UK (3)
- Harvard University (1)
- Helda - Digital Repository of University of Helsinki (6)
- Indian Institute of Science - Bangalore - Índia (6)
- National Center for Biotechnology Information - NCBI (12)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (6)
- Portal de Revistas Científicas Complutenses - Espanha (4)
- Publishing Network for Geoscientific & Environmental Data (6)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (50)
- Queensland University of Technology - ePrints Archive (97)
- Repositório digital da Fundação Getúlio Vargas - FGV (4)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (26)
- Research Open Access Repository of the University of East London. (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- School of Medicine, Washington University, United States (2)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (1)
- Universidad de Alicante (1)
- Universidad Politécnica de Madrid (4)
- Universidade Complutense de Madrid (1)
- Universidade Técnica de Lisboa (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (2)
- University of Connecticut - USA (1)
- University of Michigan (176)
- University of Queensland eSpace - Australia (28)
- University of Washington (1)
- WestminsterResearch - UK (5)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Ribotoxins are cytotoxic members of the family of fungal extracellular ribonucleases best represented by RNase T1. They share a high degree of sequence identity and a common structural fold, including the geometric arrangement of their active sites. However, ribotoxins are larger,with a well-defined N-terminal β-hairpin, and display longer and positively charged unstructured loops. These structural differences account for their cytotoxic properties.Unexpectedly, the discovery of hirsutellin A (HtA), a ribotoxin produced by the invertebrate pathogen Hirsutella thompsonii, showed how it was possible to accommodate these features into a shorter amino acid sequence. Examination of HtA N-terminal β-hairpin reveals differences in terms of length, charge, and spatial distribution. Consequently,four different HtA mutants were prepared and characterized. One of them was the result of deleting this hairpin [Δ(8-15)] while the other three affected single Lys residues in its close spatial proximity (K115E, K118E, and K123E). The results obtained support the general conclusion that HtA active site would show a high degree of plasticity,being able to accommodate electrostatic and structural changes not suitable for the other previously known larger ribotoxins, as the variants described here only presented small differences in terms of ribonucleolytic activity and cytotoxicity against cultured insect cells.