Tonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat-inactivated Mycobacterium bovis

Mycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (Sus scrofa), to evaluate the use of several enzyme-linked immunosorbent assays (ELISAs) and lateral flow tests for in vivo TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-β), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in regions that are endemic for M. bovis and for TB vaccine research.

Narrativa de tradición oral maya-tojolabal

For this research, a literary study of the oral tradition in the Maya-Tojolabal culture was carried out through an examination of a collection of 37 texts. The texts were audiovisually recorded through interviews with 17 Tojolabal informants from six communities in the state of Chiapas, Mexico. The Maya-Tojolabal people, found in the southeast of Chiapas, speak one of the Mayan languages of Mexico. One of their cultural expressions is traditional narrative, which is constituted by several versions of stories that men and women with expertise in traditional storytelling maintain alive. However, this oral tradition has been neglected by academia, and the very few studies on this tradition are primarily focused on either the historical, anthropological or linguistic aspects. The literary approach to this phenomenon has largely been relegated to articles that do not offer the possibility of any critical formalsemantic study of the stories that they publish. Nonetheless, there are some exceptional cases, which are considered in detail in this research. Like any collective art, the narrative in the Tojolabal oral tradition manifests in various forms, meanings, and mechanisms that are shared by all members of the community. In addition to being an artistic expression, the narratives are also a reflection of the values and beliefs shared by the group...