Characterization of plasmids in a human clinical strain of Lactococcus garvieae

The present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25) encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen.

La santidad compartida: la encrucijada del Islam y la ortodoxia cristiana en los Balcanes, reflejos en Bulgaria

In this doctoral thesis with the title: “The Shared Holiness: The crossroad of Islam and the Christian Orthodoxy in the Balkans, reflections in Bulgaria”, presented by VLADISLAVA SPASOVA ILIEVA, under the direction of Dr. MONTSERRAT ABUMALHAM MAS, and Dr. PEDRO BÁDENAS DE LA PEÑA, we reflect upon the crossroads between the populations in the Balkans, whose conversion into States was significantly influenced by their belonging to the Byzantine and the Ottoman Empires. Known as the crossroads of several cultures, the area was the destination of a massive influx of pagan Slaves, as well as the meeting point of Islam and Christianity. Considering the bidirectionality of the processes, we shall focus on the mutual enrichment brought about in the encounter between the religiosities of the Balkans, and we will discuss these points from different perspectives. In the common Balkan-Anatolian space, we turn our attention to the ancient hermits, with the aim of showing the complicity in the relations of coexistence, whether Islamic-Christian, Turkish-Byzantine, Turkish-Bulgarian, or Ottoman-Balkan, and a possible scheme, that could be valid for both social and spiritual growth of the person, as well as for the development of an ethnic or religious community, when sharing the same space with other populations, communities or ethnicities...