Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans

Methicillin-resistant Staphylococcus aureus (MRSA) Sequence Type (ST)1, Clonal Complex(CC)1, SCCmec V is one of the major Livestock-Associated (LA-) lineages in pig farming industry in Italy and is associated with pigs in other European countries. Recently, it has been increasingly detected in Italian dairy cattle herds. The aim of this study was to analyse the differences between ST1 MRSA and methicillin-susceptible S. aureus (MSSA) from cattle and pig herds in Italy and Europe and human isolates. Sixty-tree animal isolates from different holdings and 20 human isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa-typing, SCCmec typing, and by micro-array analysis for several virulence, antimicrobial resistance, and strain/host-specific marker genes. Three major PFGE clusters were detected. The bovine isolates shared a high (≥90% to 100%) similarity with human isolates and carried the same SCCmec type IVa. They often showed genetic features typical of human adaptation or present in human-associated CC1: Immune evasion cluster (IEC) genes sak and scn, or sea; sat and aphA3-mediated aminoglycoside resistance. Contrary, typical markers of porcine origin in Italy and Spain, like erm(A) mediated macrolide-lincosamide-streptograminB, and of vga(A)-mediated pleuromutilin resistance were always absent in human and bovine isolates. Most of ST(CC)1 MRSA from dairy cattle were multidrug-resistant and contained virulence and immunomodulatory genes associated with full capability of colonizing humans. As such, these strains may represent a greater human hazard than the porcine strains. The zoonotic capacity of CC1 LA-MRSA from livestock must be taken seriously and measures should be implemented at farm-level to prevent spill-over.

En torno a la imagen literaria de Alfonso V de Aragón: Fortún García de Ercilla y su traducción castellana del De dictis de Antonio Beccadelli. Edición y estudio

Alfonso V of Aragon (1396-1458), who won from his contemporaries the title “the Magnanimous”, became one of the most brilliant fifteenth century monarchs, not only because of being a shrewd politician and king of one of the main kingdoms in the Iberian Peninsula, but also due to his cultural activity. Thanks to him the Aragonese territories were extended throughout the Mediterranean up to Naples, where he established a magnificent court that turned into maybe the most remarkable centre of intellectual vitality and development of Humanism. His patronage attracted a considerable number of leading poets of the period, as well as the most important Italian humanists. The presence of so many writers and outstanding scholars, together with the academic environment that the monarch encouraged, promoted an enormous literary production in four languages: Latin, Spanish, Catalan and Italian. Additionally, the valuable library gathered by the king and the Academy founded in order to spread knowledge illustrate part of his intellectual concerns. This way, through his love to literature and generosity to men of letters, Alfonso the Magnanimous boosted the culture of that time. The principal protagonist in the cultural activities of the circle of erudites formed around the sovereign was Antonio Beccadelli, called Panormita (1394-1471). He, one of the most prominent personalities of Italian Humanism, assumed the role of main royal advisor. His work De dictis et factis Alphonsi regis (The sayings and deeds of king Alfonso), which will be studied in our dissertation, became a very popular text about Alfonso’s personality, as a kind of biography based on anecdotes of the Magnanimous’ life by way of exempla to be imitated. The success of these episodes lasted for a long time and they are appreciated even nowadays. The work was valued as specula principum and had great impact in sixteenth century, when De dictis was republished several times and translated from Latin into Spanish. One of these translations, the one by Fortún García de Ercilla, caught our interest since it is in a manuscript signed by Ercilla himself and this version is still unpublished...