Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans

Methicillin-resistant Staphylococcus aureus (MRSA) Sequence Type (ST)1, Clonal Complex(CC)1, SCCmec V is one of the major Livestock-Associated (LA-) lineages in pig farming industry in Italy and is associated with pigs in other European countries. Recently, it has been increasingly detected in Italian dairy cattle herds. The aim of this study was to analyse the differences between ST1 MRSA and methicillin-susceptible S. aureus (MSSA) from cattle and pig herds in Italy and Europe and human isolates. Sixty-tree animal isolates from different holdings and 20 human isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa-typing, SCCmec typing, and by micro-array analysis for several virulence, antimicrobial resistance, and strain/host-specific marker genes. Three major PFGE clusters were detected. The bovine isolates shared a high (≥90% to 100%) similarity with human isolates and carried the same SCCmec type IVa. They often showed genetic features typical of human adaptation or present in human-associated CC1: Immune evasion cluster (IEC) genes sak and scn, or sea; sat and aphA3-mediated aminoglycoside resistance. Contrary, typical markers of porcine origin in Italy and Spain, like erm(A) mediated macrolide-lincosamide-streptograminB, and of vga(A)-mediated pleuromutilin resistance were always absent in human and bovine isolates. Most of ST(CC)1 MRSA from dairy cattle were multidrug-resistant and contained virulence and immunomodulatory genes associated with full capability of colonizing humans. As such, these strains may represent a greater human hazard than the porcine strains. The zoonotic capacity of CC1 LA-MRSA from livestock must be taken seriously and measures should be implemented at farm-level to prevent spill-over.

Génesis y desarrollo de las asociaciones agrícolas y de propietarios territoriales en España

Since relatively a few years ago research work regarding the genesis and evolution of agrarian organizations in our country has been carried out. Studies with a marked regional emphasis that, rarely were able to reference the true state of the associational framework during the nineteenth century, exception made of the Sociedades Económicas and of very concrete monographic studies. It is necessary to go back to the first years of the past century to find the first descriptions and working papers alluding the agrarian associational framework in Spain. On this respect, José Elías de Molins already did a study entitled La Asociación y Cooperación Agrícolas, published in the year of 1912, in which a recount of confraternities and brotherhoods verified by the Consejo de Castilla in 1770 was done. The first of modern references related to the agrarian organizations, properly speaking, corresponds to Juan Pan-Montojo in his well known work “La naissance des associations agraries en Espagne 1833-1898”, in which the consequences and changes effected in economic power structures were reflected, once the suppression of privileges linked to the land had started, as a consequence of the strengthening of liberalism in a large portion of the state’s farms. After him, came he important works of, among others, Jordi Planas and Germán Rueda. On a comparative level, the formation of agrarian societies with mobilization capacity in other European states certainly took the same amount of time to happen. Thus, the Société des Agriculteurs de France or the Société Nationale et Centrale d’Agriculture, in 1867 and 1871, respectively, came to remind the Sociedades Económicas de Amigos del País in Spain...