Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus

The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.

Differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers

We have cloned and characterized the full-length cDNA encoding thyroid-stimulating hormone beta-subunit (TSHbeta) from orange-spotted grouper Epinephelus coioides. It contains 913 nucleotides with an open reading frame encoding 146 amino acids with a 20 amino acid signal peptide. The grouper mature TSHbeta has 75, 70, 61, 59, 41, 42 and 40% identities to that of rainbow trout, Atlantic salmon, zebrafish, European eel, chicken. mouse and human, respectively. RT-PCR analysis indicated that the TSHbeta mRNA was expressed abundantly not only in pituitary but also in gonads. A more interesting finding is to reveal the differential TSHbeta expressions between the ovaries and the transitional gonads or testes in natural individuals of orange-spotted grouper and red-spotted grouper Epinephelus akaara, and in artificial sex reversal individuals of red-spotted grouper induced by MT feeding. In situ hybridization localization provided direct evidence that the TSHbeta was transcribed in the germ cells. In the growing oocytes, the TSHbeta transcripts were concentrated on the ooplasm periphery. In testicular tissues, the intensively expressed TSHbeta cells were found to be spermatogonia and spermatocytes in the spermatogenic cysts. This is the first report of a TSHbeta expressed in the gonads of any vertebrates in addition to the expected expression in the pituitary, and it expresses more transcripts in the gonads during sex reversal or testis than in the ovaries both in E. coioides and E. akaara. Importantly, the TSHbeta identification in germ cells allows us to further investigate the functional roles and the molecular mechanisms in gametogenesis of groupers, especially in sex reversal and in spermatogenesis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

Shear Localization In Dynamic Deformation: Microstructural Evolution

Investigations made by the authors and collaborators into the microstructural aspects of adiabatic shear localization are critically reviewed. The materials analyzed are low-carbon steels, 304 stainless steel, monocrystalline Fe-Ni-Cr, Ti and its alloys, Al-Li alloys, Zircaloy, copper, and Al/SiCp composites. The principal findings are the following: (a) there is a strain-rate-dependent critical strain for the development of shear bands; (b) deformed bands and white-etching bands correspond to different stages of deformation; (c) different slip activities occur in different stages of band development; (d) grain refinement and amorphization occur in shear bands; (e) loss of stress-carrying capability is more closely associated with microdefects rather than with localization of strain; (f) both crystalline rotation and slip play important roles; and (g) band development and band structures are material dependent. Additionally, avenues for new research directions are suggested.

植被保持水土的基本规律和总结

在多年研究工作的基础上 ,全面阐述了植被保持水土的基本特征和规律 ,并对其进行了系统研究总结 ,为客观分析和评价植被的作用和效果 ,以及正确制定和实施水土保持方针和政策提供了科学依据。

Geochemical characteristics of nitrogen in the southern Yellow Sea surface sediments

Roles and distributions of various forms of nitrogen in biogeochemical cycling in the southern Yellow Sea surface sediments were investigated. The southern Yellow Sea could be divided into three regions (I, II and III) according to the proportion of fine-grained sediment in > 65%, 35-65% and < 35%, respectively. The ratios of different forms of nitrogen contents between each two of the three regions indicated that the nitrogen contents increased with the proportion of fine-grained sediment increasing. The quanta of exchangeable forms of nitrogen were similar in the three regions, while their releasing time increased from regions I to III, indicating that the cycle of nitrogen in fine-grained sediments was shorter than that in coarse-grained sediments. Nitrogen burial fluxes were also similar in these regions, while the burial efficiency increased from regions I to III. The highest burial efficiency was 30.21% in region III, indicating that more than 70% of nitrogen in the southern Yellow Sea surface sediments could be released to take part in biogeochemical recycling. When all the four forms of exchangeable nitrogen (nitrogen in ion exchangeable form (IEF-N), nitrogen in weak acid extractable form (WAEF-N), nitrogen in strong alkali extractable form (SAEF-N) and nitrogen in strong oxidant extractable form (SOEF-N)) were released to take part in recycling, their potential contributions were 80% (SOEF-N), 11% (IEF-N), 6% (SAEF-N), 3% (WAEF-N) respectively, which showed that SOEF-N was the predominant one, and its contribution to biogeochemical cycling was the highest. (c) 2004 Elsevier B.V. All rights reserved.

Geochemieal records of phosphorus in Jiaozhou Bay sediments - Implications for environmental changes in recent hundred years

Phosphorus is a key element and plays an important role in global biogeochemical cycles. The evolution of sedimentary environment is also influenced by phosphorus concentrations and fractions as well as phosphate sorption characteristics of the marine sediments. The geochemical characteristics of phosphorus and their environmental records were presented in Jiaozhou Bay sediments. Profiles of different forms of phosphorus were measured as well as the roles and vertical distributions of phosphorus forms in response to sedimentary environment changes were investigated. The results showed that inorganic phosphorus ( IP) was the major fraction of total phosphorus ( TP); phosphorus which is bound to calcium, iron and occluded phosphorus, as well as the exchangeable phosphorus were the main forms of IP, especially calcium-phosphorus, including detrital carbonate-bound phosphorus ( Det - P) and authigenic apatite-bound phosphorus ( ACa - P), are the uppermost constituent of IP in Jiaozhou Bay sediments. Moreover, the lead-210 chronology technology was employed to estimate how much phosphorus was buried ultimately in sediments. And the research showed that the impacts of human activities have increased remarkably in recent years especially between the 1980s and 2000. According to research, the development of Jiaozhou Bay environment in the past hundred years can be divided into three stages; (I) before the 1980s characterized by the relatively low sedimentation rate, weak land-derived phosphorus inputs and low anthropogenic impacts; (2) from the 1980s to around 2000, accelerating in the 1990s, during which high sedimentation rates, high phosphorus abundance and burial fluxes due to the severe: human activities impacted on the whole environmental system; (3) after 2000, the period of the improvement of environment, the whole system has been improved including the decreasing sedimentation rates, concentration and the burial fluxes of phosphonas.

投射性空间关系表达过程的研究

It is easy to find that, in each language, the terms and phrases for the representation of spatial locating and orientation, and the ways for sharing spatial knowledge are very rich. The basic way of sharing spatial information is mapping our experience and actions with the environment by using terms and utterances that represent spatial relations. How to build the mapping relation among them and what factors affect the process of mapping are the questions need to be answered in this study. The whole course of expressing projective spatial relation includes the verbal expression and perception to the projective spatial relation. In experiment 1, the perceptual characteristics of perceiving the projective spatial relation was studied by analyzing the production latencies from the presentation of the stimulators in different directions (at 5 levels: 00, 22.50, 450, 67.50, and 900) to the onset of the corresponding buttons triggering on the keyboard, the study verifies the results of prior researches and revealed the foundation of expressing the projective spatial relation. In the experiment 2, and 3, the way and the role of the verbal expression were investigated. Subjects were asked to speak out the spatial relation between intended object and reference object by using verbal locative expressions. In experiment 2, Chinese was used as the verbal expression way, and in Experiment 3, English instead. Experiment 4 was similar as experiment 3, but time of voice key triggering was controlled and balanced among trials to verify the results of Experiment 3 further. Experiment 5 investigated the effect of pre-cue on the courses of expressing projective spatial relation. There were two kinds of clues, one was the spatial locative utterances, and the other was the perceptual coordinates framework, such as drawing a cross ”+” in a circle to imply four quadrants. The main conclusions of this research were as follows: 1. When speaking out a spatial relation, different sets of spatial terms, such as “left and right”, or “north and south”, affected the speed of verbal expression. Verbal coding process was affected by how well the perceptual salient direction matched with spatial terms, which made the speed of verbal expression different. 2. When using composite spatial terms to express diagonal directions, people tend to use direct mapping from spatial conceptual representation to composite spatial terms, rather than combining the two axes, which implied there existed direct one-on-one mapping between spatial conceptual representation and spatial terms. But during specific developing period, the way of combining two axes was employed as well for spatial expression, which meant perceptual salient directions played critical role in the process of perceiving and expressing projective spatial relations. 3. The process of verbal expression of the projective spatial relation was improved by the familiarity of spatial utterances, but this improvement was not the results of enhancement of the effect of prototypical diagonal direction.

领导行为的复杂性、角色结构及其有效性研究

As the social and organizational environment become more and more complex, the topic of leadership complexity is gaining more and more attention. So far, some critical issues in this field need further exploration, such as clarifying the theoretical framework, developing and validating the measurments and exploring the mechanism of the its effectiveness. Using BEI(Behavioral Event Interview), content analysis and EFA/CFA, ANOVA, regression analysis and other qualitative/quantitative methods, this research explored the leadership structure of Chinese enterprise managers, developed a new leadership questionnaire, investigated the differences of the leadership roles among various managerial areas and on different hirachical levels, and examined the impacts of the leadership roles and leadership complexity on different indicators of leadership effectiveness in various organizational contexts. 1,020 managers were surveyed. The followings are the main findings： First，the structure of leadership behaviors of Chinese enterprise managers included ethical model, authoritarian, producer, director, monitor, mentor, strategist, enterpriser, among which ethical model and authoritarian are the new findings in Chinese cultural context. Ethical model was characterized by presenting honesty, setting an example to others, being just and diligent. Authoritarian was characterized by showing power and arbitrariness. In addition, mentor, strategist and enterpriser incarnated some cultural features of present China. The new developed leadership questionnaire’s reliability and validity reached the criterion of standardized measurement. Second, there were significant differences of frequency of leadership behaviors among the managers at different managerial postions and hirachical levels, while the impacts of different leadership roles on different leadership effectiveness indicators were also singnificantly different. Ethical model had positive impacts on the whole performance and three indicators across task and context performance, and authoritarian’s impacts on the whole performance and department performance were negatively significant. Third, the impact of leadership complexity on the whole leadership effectiveness was positively significant, while the moderating effects of organization level and position function was not significant.