In vitro anti-HIV and -HSV activity and safety of sodium rutin sulfate as a microbicide candidate

Sodium rutin sulfate (SRS) is a sulfated rutin modified from the natural flavonol glycoside rutin. Here, we investigated its in vitro anti-HIV and -HSV activities and its cytotoxic profile. Fifty percent inhibitory concentration (IC50) values of SRS against HIV-1 X4 virus IIIB, HIV-1 R5 isolates Ada-M and Ba-L were 2.3 +/- 0.2, 4.5 +/- 2.0 and 8.5 +/- 3.8 mu M with a selectivity index (SI) of 563, 575 and 329, respectively. Its IC50 against primary R5 HIV-1 isolate from Yunnan province in China was 13.1 +/- 5.5 mu M, with a Sl of 197. In contrast, unsulfated rutin had no activity against any of the HIV-1 isolates tested. Further study indicated that SRS blocked viral entry and virus-cell fusion likely through interacting with the HIV- I envelope glycoprotein. SRS also demonstrated some activity against human herpes simplex virus (HSV) with an IC50 of 88.3 +/- 0.1 mu M and a Sl of 30. The 50% cytotoxicity concentration (CC50) of SRS was >3.0 mM, as determined in human genital ME 180, HeLa and primary human foreskin fibroblast cells. Minimum inhibitory concentration of SRS for vaginal lactobacilli was >3.0 mM. These results collectively indicate that SRS represents a novel candidate for anti-HIV-1/HSV microbicide development. (C) 2007 Elsevier B.V. All rights reserved.

Safety of tetanus toxoid in pregnant women: A hospital-based case-control study of congenital anomalies

Reported are the results of the Latin American Collaborative Study of Congenital Malformations (ECLAMC), a hospital-based case-control study of 34 293 malformed and 34 477 matched nonmalformed newborn controls. No statistical differences were found between the malformed and control groups, exposed or not exposed to tetanus toroid.

龙游石窟洞室群稳定性分析及最佳旅游路线选择

The large ancient underground rock caverns in Longyou is an important component of grotto cultural. Current task facing the long-term preservation of these unmovable cultural relics is arduous and challenging. The deformation failure of the caverns' surrounding rock is deteriorating. The weathering velocity of these caverns is accelerating. With the strength of caverns' surrounding rock worsening, critical rocks were generated in local regions of the caverns' vault and posing a threat to the security of people passing by. Selection of a maximum-security route and construction a aisle in the caverns might be an efficient way to ensure the security of tourists and reach the target of long-term preservation. The deformation and destruction of the ancient underground caverns is primarily dominated by geological conditions and the special structure of caverns. Based on field investigation, several fundamental conditions for deformation and failure are recognized, and nine deformation and fracture patterns of the Longyou grotto are proposed. In order to judge the stability of caverns’ surrounding rock, the element safety coefficient method is presented. An explicit explanation for the meaning of the method is deduced using Mohr-Coulomb strength criterion. Numerical analyses are carried out in the dissertation through FLAC3D code. Through numerical analysis, the stress distribution regularities of the caverns’ roofs, piles and public side wall are analysed, and the stability properties of caverns’ surrounding rock are also assessed. At the same time, the element safety coefficient method is introduced to contrast the stability degree of different regions in caverns. The above analyses are bases for choosing the optimal tourism routes in the caverns of Longyou grotto. The impact of surface load on the stability of shallow buried cavities in Longyou grotto is evaluated, the results show that building load has significant influence on the stability of the No.1 cavern’s roof, pile and public side wall between the No.1 cavern and the No.2 cavern, pedestrian load has less impact on the stability of surrounding rock than building load. The principles for choosing the optimal tourism routes in the caverns are discussed. With these principles, the dissertation makes a systematic research on the geological analytic method, numerical analytic method and meeting tourism requirements method, which are used in selecting the optimal tourism routes in the caverns. In order to achieve the best effect in the process of tourism routes selection, the above three method are integrated through Theory of Engineering Geomechanics Meta-system(EGMS). According to field investigations, numerical analyses, tourism requirements and expert experiences, the optimal tourism routes through No.1 to No.5 cavern are determined preliminarily. The obtained results from the research work are useful for the security aisle's construction, they also have reference value to other projects in practice.