Cooperation of Mtmr8 with PI3K Regulates Actin Filament Modeling and Muscle Development in Zebrafish

Background: It has been shown that mutations in at least four myotubularin family genes (MTM1, MTMR1, 2 and 13) are causative for human neuromuscular disorders. However, the pathway and regulative mechanism remain unknown. Methodology/Principal Findings: Here, we reported a new role for Mtmr8 in neuromuscular development of zebrafish. Firstly, we cloned and characterized zebrafish Mtmr8, and revealed the expression pattern predominantly in the eye field and somites during early somitogenesis. Using morpholino knockdown, then, we observed that loss-of-function of Mtmr8 led to defects in somitogenesis. Subsequently, the possible underlying mechanism and signal pathway were examined. We first checked the Akt phosphorylation, and observed an increase of Akt phosphorylation in the morphant embryos. Furthermore, we studied the PH/G domain function within Mtmr8. Although the PH/G domain deletion by itself did not result in embryonic defect, addition of PI3K inhibitor LY294002 did give a defective phenotype in the PH/G deletion morphants, indicating that the PH/G domain was essential for Mtmr8's function. Moreover, we investigated the cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development, and found that both Mtmr8-MO1 and Mtmr8-MO2+LY294002 led to the disorganization of the actin cytoskeleton. In addition, we revealed a possible participation of Mtmr8 in the Hedgehog pathway, and cell transplantation experiments showed that Mtmr8 worked in a non-cell autonomous manner in actin modeling. Conclusion/Significance: The above data indicate that a conserved functional cooperation of Mtmr8 with PI3K regulates actin filament modeling and muscle development in zebrafish, and reveal a possible participation of Mtmr8 in the Hedgehog pathway. Therefore, this work provides a new clue to study the physiological function of MTM family members.

Geometric phase of a bipartite system with Dzyaloshinski-Moriya interaction

The Berry phase of a bipartite system described by a Heisenberg XXZ model driven by a one-site magnetic field is investigated. The effect of the Dzyaloshinski-Moriya (DM) anisotropic interaction on the Berry phase is discussed. It is found that the DM interaction affects the Berry phase monotonously. and can also cause sudden change of the Berry phase for some weak magnetic field cases. (c) 2008 Elsevier B.V. All rights reserved.

基于MDA的TRISO-Model模型管理方法及应用

TRISO-Model(tridimensional integrated software development model)是为处理软件开发的复杂性和动态性而提出的三维集成软件开发方法学,其中,多维模型之间的语义一致性维护以及对模型应用中公共操作部分的重用,提出了基于一致语义进行模型管理的需求.给出了基于MDA(model driven architecture)进行模型管理的方法MDA-MMMethod(MDA based model management method),应用MDA的4层模型管理结构,基于MDA核心标准MOF(meta object facility)所提供的公共语义基础管理模型和元模型,MDA-MMMethod支持各种MDA模型操作标准实现在TRSIO-model应用中的重用.开发了相应的支持系统MDA-MMSystem(MDA based model management system),应用于SoftPM的项目实践中.与传统方法相比,模型应用的开发效率得到了显著提高,同时降低了开发成本.最后,给出了模型融合的应用实例介绍.

基于模型驱动的实时数据发布系统研究与应用

流程企业生产过程反映了企业的工艺路线、资源配置及其制造能力，生产过程的稳定运行影响着产品生命周期的各个阶段，因此进行生产过程的实时监控非常必要。生产过程中的大量装置所包含的静态数据和实时的动态数据构成了生产过程监控的数据基础，因此实现生产过程的实时监控依赖于基于装置对象的生产过程的快速配置。 本文以国家高技术研究发展计划（863计划）课题“面向全流程多尺度的综合自动化集成应用平台”为背景，针对流程企业生产过程监控的具体需求，研究并开发了基于模型驱动的实时数据发布系统，实现了基于装置对象的生产过程的快速配置。论文主要工作如下： 1.给出了基于数据驱动机制的装置对象描述模型，建立了面向流程企业生产过程的装置资源库。在此基础上给出了一种基于装置对象描述模型的生产过程快速配置方法。 2.针对流程企业生产过程监控的具体需求，引入XML-QL与复合事件的时序逻辑模型，提出了一个基于XML和复合事件的发布/订阅模型，给出了基于订阅树的复合事件匹配算法。最后在生产过程模型的基础上设计了一个实时数据发布框架。 3.基于.NET平台，设计开发了基于装置对象模型驱动的实时数据发布系统，并基于某石化企业的核心数据进行了应用验证，取得了良好的效果。 关键词：生产过程监控；模型驱动；发布/订阅；复合事件

Model 75图象处理系统止软件开发方法的研究

Model 75是一个先进的图象处理机，它大量采用了先进的硬件技术，从而使其具有一般通用机无法相比的强大处理能力。System 575是运行于Model 75上的图象处理软件系统。它有比较完善的图象处理能力，然而System 575却没有一种好的软件开发方法，致使我所购进Model 75以来没能充分利用它的强大能力。针对这种情况，本文探讨了一种能自由运用Model 75的软件开发方法。由于Model 75不同于一般的通用机，因而很好地使用Model 75必须建立在对它的结构有清楚的了解的基础上。本文在大量实验的基础上对Model 75的结构及功能特点做了分析。为验证本人对Model 75的理解和本文所述方法的正确性，本人做了两个较大的实验，一个是运动模糊图象的恢复，一个是道路寻找的可变此值方法。本文介绍了这两个实验。实验表明本文所述的开发方法是有效的，解决了在Model 75上开发软件的难题。

一种TEC地图构建方法与全球电子总含量的气候学研究

Global positioning system (GPS) can not only provide precise service for navigation and timing, but also be used to investigate the ionospheric variation. From the GPS observations, we can obtain total electron content (TEC), so-called GPS TEC, which is used to characterize the ionospheric structure. This thesis mainly concerns about GPS TEC data processing and ionospheric climatological analysis as follows. Firstly, develop an algorithm for high-resolution global ionospheric TEC mapping. According to current algorithms in global TEC mapping, we propose a practical way to calibrate the original GPS TEC with the existing GIM results. We also finish global/local TEC mapping by model fitting with the processed GPS TEC data; in practice, we apply it into the local TEC mapping in Southeast of China and obtain some initial results. Next, suggest a new method to calculate equivalent ionospheric global electron content (GEC). We calculate such an equivalent GEC with the TEC data along the geographic longitude 120°E. With the climatological analysis, we can see that GEC climatological variation is mainly composed of three factors: solar cycle, annual and semiannual variations. Solar cycle variation is dominant among them, which indicates the most prominent influence; both annual and semiannual variations play a secondary role and are modulated by solar activity. We construct an empirical GEC model driven by solar activity and seasonal factors on the basis of partial correlation analysis. Generally speaking, our researches not only show that GPS is advantageous in now-casting ionospheric TEC as an important observation, but also show that GEC may become a new index to describe the solar influence on the global ionosphere since the great correlation between GEC and solar activity factor indicates the close relationship between the ionosphere and solar activity.

塔里木盆地轮南地区三叠系油气地质模型及油气预测研究

As an important measure to understand oil and gas accumulation during petroleum exploration and development, Petroleum geological model is an integrated system of theories and methods, which includes sedimentology, reservoir geology, structural geology, petroleum geology and other geological theories, and is used to describe or predict the distribution of oil and gas. Progressive exploration and development for oil and gas is commonly used in terrestrial sedimentary basin in China for the oil and gas generation, accumulation and exploitation are very intricate. It is necessary to establish petroleum geological model, adaptive to different periods of progressive exploration and development practice. Meanwhile there is lack of an integrated system of theories and methods of petroleum geological model suitable for different exploration and development stages for oil and gas, because the current different models are intercrossed, which emphasize their different aspects. According to the characteristics of exploration and development for the Triassic oil and gas pool in Lunnan area, Tarim Basin, the Lunnan horst belt was selected as the major study object of this paper. On the basis of the study of petroleum geological model system, the petroleum geological models for different exploration and development stages are established, which could be applied to predict the distribution of oil and gas distribution. The main results are as follows. (1) The generation-accumulation and exploration-development of hydrocarbon are taken as an integrated system during the course of time, so petroleum exploration and development are closely combined. Under the guidance of some philosophical views that the whole world could be understood, the present writer realizes that any one kind of petroleum geological models can be used to predict and guide petroleum exploration and development practice. The writer do not recognize that any one kind of petroleum geological models can be viewed as sole model for guiding the petroleum exploration and development in the world. Based on the differences of extents and details of research work during various stage of exploration and development for oil and gas, the system of classification for petroleum geological models is established, which can be regarded as theoretical basis for progressive petroleum exploration and development. (2) A petroleum geological model was established based on detailed researches on the Triassic stratigraphy, structure, sedimentology and reservoir rocks in the Lunnan area, northern Tarim Basin. Some sub-belt of hydrocarbon accumulation in the Lunnan area are divided and the predominate controlling factors for oil and gas distribution in the Lunnan area are given out. (3) Geological models for Lunnan and Jiefangqudong oil fields were rebuilt by the combinations of seismology and geology, exploration and development, dynamic and static behavior, thus finding out the distribution of potential zones for oil and gas accumulations. Meanwhile Oil and gas accumulations were considered as the important unit in progressive exploration and development, and the classification was made for Lunnan Triassic pools. Petroleum geological model was created through 3D seismic fine interpretation and detailed description of characteristics of reservoir rocks and the distribution of oil and gas, especially for LN3 and LN26 well zones. The possible distribution of Triassic oil traps and their efficiency in the Lunnan area has been forecasted, and quantitative analysis for original oil(water) saturation in oil pools was performed. (4) The concept of oil cell is proposed by the writer for the first time. It represents the relatively oil-rich zones in oil pool, which were formed by the differences of fluid flows during the middle stage of reservoir development. The classification of oil cells is also given out in this paper. After the studies of physical and numerical modeling, the dominant controlling factors for the formation of various oil cells are analyzed. Oil cells are considered as the most important hydrocarbon potential zones after first recovery, which are main object of progressive development adjustment and improvement oil recovery. An example as main target of analysis was made for various oil cells of Triassic reservoir in the LN2 well area. (5) It is important and necessary that the classification of flow unit and the establishment of geological model of flow unit based on analysis of forecast for inter-well reservoir parameters connected with the statistical analysis of reservoir character of horizontal wells. With the help of self-adaptive interpolation and stochastic simulation, the geological model of flow units was built on the basis of division and correlation of flow units, with which the residual oil distribution in TIII reservoir in the LN2 well area after water flooding can be established.

Multiscale Treatment of Thin-Film Lubrication

A multiscale technique that combines an atomistic description of the interfacial (near) region with a coarse-grained (continuum) description of the far regions of the solid substrates is proposed. The new hybrid technique, which represents an advance over a previously proposed dynamically-constrained hybrid atomistic-coarse-grained treatment (Wu et al.J. Chem. Phys., 120, 6744, 2004), is applied to a two-dimensional model tribological system comprising planar substrates sandwiching a monolayer film. Shear–stress profiles (shear stress versus strain) computed by the new hybrid technique are in excellent agreement with “exact” profiles (i.e. those computed treating the whole system at the atomic scale).

A special method to evaluate the strong adhesion between brittle coating and ductile substrate

The evaluation of the interfacial adhesion of coating system has always been a rough task. In this paper, a special testing method of cross-sectional indentation is applied on a model coating system, i.e. electroplated chromium on a steel substrate which is generally regarded as an example of materials pair with strong adhesion. Based on fractography analysis with SEM and interfacial stress simulation with FEM, it is found that interfacial shear stress may induce coating spalling. More interestingly, spalling location is sensitive to substrate pretreatment process. This shows the feasibility of cross-sectional indentation to distinguish interfacial strength at a high level.

Interface Fracture Behavior Of Electroplated Coating On Metal Substrate Under Compressive Strain

The inducement of interface fracture is crucial to the analysis of interfacial adhesion between coating and substrate. For electroplated coating/metal substrate adhering materials with strong adhesion, interface cracking and coating spalling are difficult to be induced by conventional methods. In this paper an improved bending test named as T-bend test was conducted on a model coating system, i.e. electroplated chromium on a steel substrate. After the test, cross-sections of the coated materials were prepared to compare the failure behaviors under tensile strain and compressive strain induced by T-bend test. And the observation results show that coating cracking, interface cracking and partial spalling appear step by step. Based on experimental results, a new method may be proposed to rank the coated materials with strong inter-facial adhesion. (C) 2008 Elsevier B.V. All rights reserved.

支持EJB动态分布的组件迁移模型与算法

Web应用服务器是Web计算环境下的新型中间件，为基于组件的分布式Web应用提供了基础运行平台．组件静态分布限制了事务性Web应用在运行期间适应执行环境变化的能力．为了满足Web应用的动态分布需求，Web应用服务器需在底层为组件提供一种动态迁移的能力．如何维持组件迁移前后的一致性是组件迁移中最棘手的问题之一．为解决此问题，定义了组件迁移一致性约束CMC(component migration constrains)，并给出了在 J2EE(Java 2 platform enterprise edition)应用服务器中支持EJB(enterprise Java Bean)动态分布的组件迁移模型和 sLB_Copy,SFB_Copy,EB-Copy 3个迁移算法．分析得出sLB_Copy,SFB_copy和EB_copy均满足CMC约束．迁移模型和算法已在自主研制的Web应用服务器WebFrame2．0中实现，并已应用到自适应负载平衡、失效恢复等多个方面．