Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency

Micro-fabrication technology has substantial potential for identifying molecular markers expressed on the surfaces of tissue cells and viruses. It has been found in several conceptual prototypes that cells with such markers are able to be captured by their antibodies immobilized on microchannel substrates and unbound cells are flushed out by a driven flow. The feasibility and reliability of such a microfluidic-based assay, however, remains to be further tested. In the current work, we developed a microfluidic-based system consisting of a microfluidic chip, an image grabbing unit, data acquisition and analysis software, as well as a supporting base. Specific binding of CD59-expressed or BSA-coupled human red blood cells (RBCs) to anti-CD59 or anti-BSA antibody-immobilized chip surfaces was quantified by capture efficiency and by the fraction of bound cells. Impacts of respective flow rate, cell concentration, antibody concentration and site density were tested systematically. The measured data indicated that the assay was robust. The robustness was further confirmed by capture efficiencies measured from an independent ELISA-based cell binding assay. These results demonstrated that the system developed provided a new platform to effectively quantify cellular surface markers effectively, which promoted the potential applications in both biological studies and clinical diagnoses.

基于意象知识的消歧体系

本文提出了一种可以表示常识及语言知识的意象知识体系。在这种知识的形式化表示基础上,给出了NLP中的消歧知识及其表示形式,以及基于消歧知识的消歧策略。最后,论述了这种方法实现上的可行性。

hyo-xtm: a set of hyper-graph operations on xml topic map toward knowledge management

Knowledge management is a critical issue for the next-generation web application, because the next-generation web is becoming a semantic web, a knowledge-intensive network. XML Topic Map (XTM), a new standard, is appearing in this field as one of the structures for the semantic web. It organizes information in a way that can be optimized for navigation. In this paper, a new set of hyper-graph operations on XTM (HyO-XTM) is proposed to manage the distributed knowledge resources.HyO-XTM is based on the XTM hyper-graph model. It is well applied upon XTM to simplify the workload of knowledge management.The application of the XTM hyper-graph operations is demonstrated by the knowledge management system of a consulting firm. HyO-XTM shows the potential to lead the knowledge management to the next-generation web.

Evaluation of a combined wavelet and a combined principal component analysis classification system for BCG diagnostic problem

Heart disease is one of the main factor causing death in the developed countries. Over several decades, variety of electronic and computer technology have been developed to assist clinical practices for cardiac performance monitoring and heart disease diagnosis. Among these methods, Ballistocardiography (BCG) has an interesting feature that no electrodes are needed to be attached to the body during the measurement. Thus, it is provides a potential application to asses the patients heart condition in the home. In this paper, a comparison is made for two neural networks based BCG signal classification models. One system uses a principal component analysis (PCA) method, and the other a discrete wavelet transform, to reduce the input dimensionality. It is indicated that the combined wavelet transform and neural network has a more reliable performance than the combined PCA and neural network system. Moreover, the wavelet transform requires no prior knowledge of the statistical distribution of data samples and the computation complexity and training time are reduced.

dynamic evolutions based on ontologies

With the continuous changes in application requirements of the enterprises, Web resources must be updated, so do the underlying ontologies that are associated with the Web resources. In the situation, it is very challenging for ontological engineers to specify the changes of ontologies, keep their consistencies and achieve semantic query of Web resources based on the evolving ontologies. We propose a construct called Prioritized Knowledge Base (PKB) based on SHOQ(D) description logic, and discuss some properties of PKB.PKB can be used for describing the evolutions and updates of ontologies with conflicting information. Furthermore, we develop some algorithms for checking conflict rules and performing semantic query based on PKB.

Structure and electrical properties of Pr ans Sm doped CeO2 and Ce2MoO15 based solid electrolytes

Sin and Pr doped CeO2 and Ce6MoO15 based materials were synthesized by sol-gel method. The structure of the powders were characterized by X-ray diffraction (XRD), Raman spectra, field emission scanning electron microscopy(FE-SEM) and the electrical conductivity of the samples was investigated by AC impedance spectroscopy. By comparing the structure and electrical properties of different systems, it could be concluded that the electrical property of Ce6MoO15 based system is better than that of CeO2 system. The added Mo element resulted in the increase of gain size and improved the grain boundary conductivity notably below 600 degrees C, while the Pr dopant induced the smaller grain size and improved the grain boundary conductivity of the materials.

基于知识的计算机辅助工艺设计系统

介绍了一个较为通用的基于知识的计算机辅助工艺设计（ＣＡＰＰ）系统结构，包括知识表达和关联函数，知识库管理，任务分解与综合策略，解释机制，与其他系统的接口。

一种基于知识的自然景物解释

利用我们研制成的基于知识的区域分析系统,我们对自然景物图象做了分析、解释。本文介绍:(1)图象初始分割;(2)图象特征提取;(3)规则集构造。这里,图象初始分割采用的是模糊域方法,它基于Fuzzy C-means 算法,并在此基础上修改了收敛准则,增加了迭代分割功能。图象特征分为主持征及从特征,它们建立在层次化的区域数据结构上。适合于区域分析的规则集已包括三类规则,它们不仅具有较强的知识表示能力,而且易于控制及利用。本文介绍针对包括天空、道路、树木、建筑物等物体的简单景物图象所做的解释实验,并给出了实验结果.

流程企业计划调度体系结构研究

以石化企业为背景,在深入分析流程企业计划调度系统结构特征的基础上,结合流程企业计划调度的关键目标,提出了一种基于模型的中心化流程企业计划调度通用结构,突出了计划调度的中心地位.以此为指导,进一步建立并实现了基于知识的智能计划调度总体结构,为流程企业生产系统的运行优化提供了方法论的指导.

Laser drilling improving circuit board manufacturing

We reported here a novel technique for laser high speed drillings on Printed Circuit Boards (PCBs). A CNC solid laser based system is developed to drill through and blind vias as an alternative to mechanical drilling. The system employs an Acousto-Optic Q-switched Nd: YAG laser, a computer control system and an X-Y moving table which can handle up to 400 x 400 mm PCB. With a special designed cavity the laser system works in a pulsed operation in order to generate pulses with width down to 0.5 mu s and maximum peak power over 10kW at 10k repetition rate. Delivered by an improved optical beam transforming system, the focused laser beam can drill hobs including blind vias on PCBs with diameter in the range of 0.1 - 0.4 mm and at up to 300 - 500 vias per second (depending on the construction of PCBs). By means of a CNC X-Y moving system, laser pulses with pulse-to-pulse superior repeatability can be fired at desired location on a PCBs with high accuracy. This alternative technology for drilling through or blind vias on PCBs or PWBs (printed wiring boards) will obviously enhance the capability to printed boards manufacturing.

facilitating formal specification acquisition by using recursive functions on context-free languages

Although formal specification techniques are very useful in software development, the acquisition of formal specifications is a difficult task. This paper presents the formal specification language LFC, which is designed to facilitate the acquisition and validation of formal specifications. LFC uses context-free languages for syntactic aspect and relies on a new kind of recursive functions, i.e. recursive functions on context-free languages, for semantic aspect of specifications. Construction and validation of LFC specifications are machine-aided. The basic ideas behind LFC, the main aspects of LFC, and the use of LFC and illustrative examples are described.

基于草图界面的应用系统框架研究与实现

现有的草图系统重在设计对象外形的表达，忽略了隐含在设计过程中用户思维活动的信息；另一方面，目前笔的交互方式尚不能自然、流畅地表达用户设计意图．通过分析草图界面信息对象和交互过程的特点，提出面向构思设计的应用系统框架ISF，以满足设计意图原型的快速生成；同时结合交互的任务、环境和用户偏好等上下文信息，建立从物理设备到逻辑工具映射的交互模型，旨在为此类系统的交互设计提供一定指导的方法．最后给出面向轻纺行业服装设计的应用实例——草图图板系统．应用结果表明，ISF能够为此类草图应用系统的设计给予一定的指导，满足自然、流畅的交互设计要求．

智能制造装备数字化设计与验证系统研究

智能制造装备的设计水平不高是制约我国装备制造业发展因素之一。在阐述智能制造装备特点的基础上,提出了一种基于CORBA规范的智能制造装备数字化设计与验证系统的体系结构,并探讨了实现该系统的关键技术。该系统的目的是降低智能制造设备开发成本,缩短开发周期,提高智能制造装备的设计质量。

基于知识的供应链决策框架

本文提出了一种基于知识的供应链决策框架,并且从理论和实践的角度详细探讨了知识积累和知识挖掘过程,充分利用知识优势,以期通过知识与推理机制的集成和人类智慧与枯燥数据之间的无缝链接来提高供应链性能。它不仅适用于供应链整体而且也适用于供应链各侧面。它可实现独立地、自动地、实时地及时决策以缩短顾客定货周期,降低库存,提高交付准确性和资源利用水平。决策框架同样也适用于其它类似的情况。