Identification of herb Acanthopanax senticosus (Rupr. Et Maxim.) harms by capillary electrophoresis with electrochemical detection

In this paper, a rapid, high efficient, sensitive and inexpensive approach based on a combination of simple ultrasonic extract and capillary electrophoresis (CE) separation with electrochemical detection (ED), is described to identify herbs by comparing their CE-ED profiles (namely, CE-ED electropherograms). The proposed method takes advantage of ultrasmall sample volume, low consumption of organic solvent, simple sample pretreatment and easy cleanup procedure. It was applied to analyze the CE-ED profiles of stems of herb Acanthopanax senticosus (Rupr. Et Maxim.) Harms from different sources and different parts (roots, rhizomes, stems and leaves) of this herb. By comparing peak number, peak height and peak height ratio, we found that the CE-ED profiles showed big differences for the herbs from the different sources and the different parts of this herb. In addition, the distribution of bioactive compounds (isofraxidin, rutin and chlorogenic acid) in the different parts of this herb and their content variations affected by the source were studied with the CE-ED method. Based on their own unique CE-ED profiles, these herbs from the different sources and the different parts of this herb could be easily distinguished. Therefore, the proposed approach could be used as a rapid, high efficient and sensitive method for the identification of herbal medicines.

Characterization of triterpenoidic saponin mixture in crude extracts from leaves of Acanthopanax senticosus Harms by saponin structural correlation and mass spectrometry

Eighteen triterpenoidic saponins in crude extracts from leaves of Acanthopanax senticous Harms have been investigated by electrospray ionization multi-stage tandem mass spectrometry and high-resolution mass spectrometry. In ESI-MS spectra, predominant [M + Na](+) ions in the positive ion mode have been observed for molecular mass information. Meanwhile, specific structural correlations between these ions are firstly found. The 18 peaks (ions) can be classified into three groups (group D, E, and F with mass increase) with each group including six peaks. There is a mass difference of 132 Da between group D and E for each corresponding peak in turn (for example peak 1 to peak 7), indicating one more pentose residue was attached to saponins in group E than those corresponding in group D. The mass difference of 146 Da between group E and F implies one more deoxy-hexose attached to saponins in group F than those corresponding in group E. The structural correlations of the corresponding ions are confirmed by tandem mass spectrometry and high-re solution mass spectrometry. These structural features can not only facilitate the rapid characterization of the native known saponins in crude plant extracts, thus avoiding tedious derivation and separation of saponins, but also help find novel compounds of the same type in a specific medicinal plant.

Analysis of flavonoids from leaves of Acanthopanax Senticosus Harms

Using electrospray tandem mass spectrometry (ESI-MSn), the flavonoids obtained from leaves in Acanthopanax Senticosus Harms were analyzed. The typical colorimetric method and the ultroviolet spectrophotometry were also utilized for the determination of the content of total flavonoids. The analytical results showed that there was quercetin as well as its derivatives in leaves of acanthopanax senticosus harms and their content was as high as 37.25%.

Identification of the flavonoid constituents from leaves of Acanthopanax Senticosus Harms

Four flavonoids from leaves of Acanthopanax Senticosus Harms were observed in negative ion mode in the electrospray mass spectra. Two of them were further isolated and identified as quercitrin (quercetin-3-O-alpha-L-rhamnoside) and hyperin (quercetin-3-O-beta-D-galactoside) on the basis of MS' and NMR data. The other two compounds in the mixtures were tentatively established as quercetin and rutin (quercetin-3-O-rutinoside) in terms of their electrospray tandem mass spectrometry (ESI-MSn) data. Three of the four flavonoids (excluding hyperin) haven't been reported in this plant before.

Analysis of flavonoid constituents from leaves of Acanthopanax senticosus Harms by electrospray tandem mass spectrometry

Three known flavonoids, quercetin, quercitrin (quercetin-3-0-rhamnoside) and rutin (quercetin-3-0-rutinoside), have been identified for the first time in the leaves of Acanthopanax senticosus Harms by using electrospray tandem mass spectrometry techniques (ESI-MSn). The flavonoid hyperin (quercetin-3-0-beta-galactoside), already known to be present, was also investigated. The diagnostic fragment ions of the aglycone quercetin were obtained in the ESI-MSn experiments, and a fragmentation mechanism proposed.

Multi-stage mass spectrometric studies of triterpenoid saponins in crude extracts from Acanthopanax senticosus harms

Three triterpenoid saponins in crude extracts from Acanthopanax senticosus Harms have been investigated by use of multi-stage mass spectrometry (MSn) combined with electrospray ionization (ESI), MSn spectra were applied to direct structure elucidation of these saponins in crude plant extracts, in positive and negative ion mode. The characteristic fragmentations of triterpenoid saponins are discussed. The method provides a means of rapid initial screening of crude plant extracts. Copyright (C) 1999 John Wiley & Sons, Ltd.

刺五加活性成分及其相关非共价复合物的质谱研究

中药化学成分，尤其是活性成分，是中药发挥药效作用的物质基础，它的深入研究是中药现代化的关键和核心。而中药成分的复杂性和药效的整体性，一般的分析及研究方法无法简捷快速地解决中药有效成分鉴定的难题，直接影响到中药研究开发的进程。质谱技术，尤其是软电离质谱及其联用技术，由于其具有灵敏、快速、分辨率高的优点，使得质谱日益成为研究复杂生物分子和中药体系中的应用问题的强有力手段。本论文以刺五加新药的开发为背景，采用有效部分的研究方法，运用先进的质谱技术，以药理活性筛选为指导，全面地研究了刺五加的活性成分。首先，采用电喷雾多极串联质谱技术，研究了正负离子条件下传统中药刺五加中的有效成分刺五加营B和刺五加营E的特征质谱行为，并提出了质谱碎裂机理，可以此鉴定传统刺五加有效成分，对传统刺五加药材及相关药品的快速鉴别和质量控制具有重要意义。以人参皂营R1为例，定量地研究四种碱金属离子浓度对皂普结构分析的影响，为合理添加碱金属离子以提高皂着分析的灵敏度提供了依据。在此基础上，用电喷雾多极串联质谱技术简便而又快捷地区分了两对生理活性差别较大的人参皂营的同分异构体：Rg1和PF11，Rg2和Rg3，为深入研究人参皂普的药效成分和作用机制奠定了一定的基础。系统地研究了β-香树”脂醇型的三种五环三菇皂营在不同模式下的多级串联质谱特征，为从多角度阐明皂昔类化合物的结构提供了新的思路。结构相近的皂普类化合物在电喷雾多级串联质谱中都表现出了与其自身结构相符的特征质谱行为，为不同皂营混合物的快速鉴定提供了一种快速有效的分析方法。在此基础上，研究了两种典型的刺五加仆卜皂营的质谱特征，并用多种质谱手段从刺五加叶总皂普中检测到18种皂普成分，其中有8种皂营成分在刺五加中而未见有文献报道，并用质谱首次发现了刺五加叶皂营的结构和其在植物体内代谢的相关性，这一结果对研究刺五加皂营的体内代谢有着十分重要的意义。以药理目标活性成分为指标，采用对比实验和一系列正交实验，对刺五加活性成分的提取方法，提取工艺和纯化工艺进行了优化，首次采用联合树脂法一次性分离纯化刺五加中两类有效成分总皂昔和总黄酮，经工艺优化最终得到总有效成分达85％以上的刺五加制剂，为刺五加新药的开发提供了大量的工艺参数。基于环糊精包合物（CDs）在中药剂型改造及提高某些药物稳定性和生物利用度方面广阔的应用前景和刺五加新药开发中的实际需要，系统到研究了三种环糊精（a-CD，β-CD和γ-CD）与刺五加皂昔元齐墩果酸（OA）形成的复合物。纬果表明，三种环糊精都能与OA形成稳定的l:1复合物，而β-CD和γ-CD还能与以形成2:1“三明治”状的复合物。复合物气液两相性质具有良好的相关性，为质谱快速筛选和表征这类非共价复合物提供了必要的条件。采用同样的方法对三种环糊精与刺五加中黄酮类化合物（芦丁和懈皮素）的复合物进行了系统研究，结果发观，三种环糊精都与芦丁（R）和棚皮素（Q）形成1:1复合物。但复合物稳定性有差异，对环糊精与芦丁的1:1复合物，以β-CD复合物最稳定，而对于环糊精与槲皮素1:1复合物则以α-CD复合物最稳定，这与芦丁含有糖链有关。刺五加活性成分黄酮类化合物生物利用度和活性的另一有效途径是制备黄酮-金属络合物。黄酮-金属络合物作为潜在的药物开发正日益受到重视，本文利用电喷雾质谱系统地研究了第四周期五种二价金属离子与棚皮素形成的络合物，并采用多级串联质谱部分表征了这些络合物的结构和稳定性之间的关系，为建立黄酮金属络合物筛选体系和表征提供了一种较好的方法，为这类新药的研制开发提供了一定的理论基础。

小麦—偏凸山羊草6Mv/6B代换系的研究

小麦条锈病（Puccinia striiformis f. sp. tritici）是世界性小麦病害，可导致受害小麦减产30%以上，甚至绝收。小麦条锈病在我国西南、华北麦区危害严重，四川麦区是小麦条锈病发病最重的地区之一，每年因条锈病流行造成小麦产量损失巨大。利用抗条锈病品种是控制该病害最安全、经济的有效途径，因此挖掘利用抗病新基因，开展抗病遗传基础研究是当前育种工作中面临的重要任务。 偏凸山羊草（Aegilops ventricosa，DDMvMv，2n=28）是一年生草本植物，起源于地中海西部沿岸地区，具有对小麦白粉病、锈病等高抗或免疫、耐盐、抗寒、蛋白质含量高等优良性状，是小麦遗传育种很好的种质资源。本研究以高抗条锈病的小麦—偏凸山羊草6Mv/6B代换系（Moisson 6Mv/6B）为材料，对其含有的带条锈病抗性基因的偏凸山羊草6Mv染色体在四川小麦背景中的传递情况、与小麦—簇毛麦双端体附加系所具有的白粉病抗性的聚合以及对Moisson 6Mv/6B进行电离辐射诱变筛选抗条锈病的小麦—偏凸山羊草易位系三个方面进行了研究。取得的主要研究结果如下： 1. Moisson 6Mv/6B与高感条锈病的四川地区普通小麦品种绵阳26、绵阳93-124和SW3243的杂种F1与其普通小麦亲本分别作为父、母本回交，通过对其BC1和F2的结实率、根尖细胞有丝分裂中期染色体的观察以及对条锈病抗性的鉴定，发现含6Mv染色体的F1植株作母本时的回交结实率（83.10%）普遍高于含6Mv染色体的F1植株作父本（48.61%），结实率与普通小麦基因型密切相关（χ2=34.15>>χ20.05=5.99（df=2））；6Mv染色体在三种四川小麦中通过雌、雄配子传递的传递方式与其传递率间没有显著相关性，其传递率与普通小麦基因型呈显著相关性（χ2=6.42>χ20.05=5.99（df=2））。 2. Moisson 6Mv/6B与高抗白粉病的小麦—簇毛麦双端体附加系Pana（2n=42+2t）正反杂交，希望在聚合两者抗性的同时观察不同受体背景下的抗性反应。对Moisson 6Mv/6B和Pana正反杂交的结实率、杂交后代的农艺性状进行观察，并对杂交后代进行基因组荧光原位杂交（GISH）分析及条锈病和白粉病的抗性鉴定。结果表明Moisson 6Mv/6B作母本时杂交结实率（80.56%）高于Pana作母本时（58.33%），结实率与杂交方式间紧密相关（χ2=4.96>χ20.05=3.84（df=1））；Moisson 6Mv/6B和Pana杂交后代株高比最高亲本高约10cm，成熟期也较两亲本提前两个星期左右；正反杂交后代中具有偏凸山羊草6Mv染色体的植株具有条锈病抗性，具有簇毛麦端体的植株具有白粉病抗性，同时筛选到4株含有偏凸山羊草和簇毛麦遗传物质并对条锈病和白粉病兼抗的材料，证明来自偏凸山羊草6Mv染色体的条锈病抗性与来自簇毛麦端体的白粉病抗性已经聚合在一起，且没有产生相互抑制的作用，暗示通过这两个抗性基因的聚合是完全能获得兼抗条锈病和白粉病的小麦新种质。 3. 对Moisson 6Mv/6B在减数分裂时期的成株进行总剂量为6Gy、辐射频率为120rad/min的60Co-γ射线辐射，对辐射植株自交后代进行农艺性状及根尖细胞有丝分裂中期染色体形态观察和条锈病抗性鉴定。结果为辐射植株自交结实率为2.22%，根尖细胞有丝分裂中期的染色体存在明显碎片，辐射自交后代植株对条锈病具有成株期抗性。 小麦—偏凸山羊草6Mv/6B代换系对条锈病抗性稳定，是培育条锈病抗性品种的良好供体。本研究证明在四川小麦背景中要利用该品种抗性，在结实数满足需要时，可将其作父本，亦可作母本，但关键是要选择好一个优良的受体基因型；同时其条锈病抗性与来自簇毛麦的白粉病抗性没有相互抑制作用，可将两者抗性有效聚合用于小麦育种中。 Wheat stripe rust (Puccinia striiformis f. sp. Tritici) is a worldwide disease of wheat, and could lead to victims of 30 percent or even total destruction of wheat production. Wheat stripe rust harms badly in China's southwest and North China. Sichuan province is one of the regions damaged by wheat stripe rust heavily. The use of resistant varieties is the most secure and economical way to control the wheat stripe rust. Therefore, it is essential to identify new disease-resistant genes and genetically research of disease resistance. Aegilops ventricosa (DDMvMv, 2n = 28) is an annual herbaceous plant, originating in the coastal areas of the western Mediterranean, with good characters such as resistance of wheat powdery, rust, salt, cold and high protein content. It is a good germplasm resource. In this study, the wheat- Aegilops ventricosa 6Mv/6B substitution line Moisson 6Mv/6B (highly resistant to the wheat stripe rust) was used to study on the transmission of chromosome 6Mv of Aegilops ventricosa in different genetic background of Sichuan wheat varieties, hybridization with wheat- Haynaldia villosa ditelosomic addition line Pana (highly resistant to the powdery mildew) and screening of wheat- Aegilops ventricosa translocation line by exposuring Moisson 6Mv/6B under ionizing radiation. The main results are as following: 1. Moisson 6Mv/6B was crossed with Sichuan wheat varieties mianyang26, mianyang93-124 and SW3243 (highly susceptible to stripe rust), respectively. Their F1 hybrids were further backcrossed as male and female to corresponding wheat varieties. The seed-setting rate, chromosomes confirmation in the mitotic metaphase of root tip cells, and resistance to stripe rust of the subsequent BC1 and F2 plants were investigated. The average seed-setting rate of backcross via 6Mv as female donor (83.10%) was higher than that of backcross via 6Mv as male donor (48.61%), suggesting that the seed-setting rate was associated with the wheat genotypes（χ2=34.15>>χ20.05=5.99（df=2））. In all analyzed populations, transmission frequencies of chromosome 6Mv were not correlated with the ways of 6Mv through male or through female. However, transmission frequencies of chromosome 6Mv were significantly correlated with Sichuan wheat genotypes（χ2=6.42>χ20.05=5.99（df=2））. 2. To aggregating the resistances to stripe rust and powdery mildew, as well as research on the resistance reactions in different genetic background, Moisson 6Mv/6B was reciprocally hybrided with the wheat- Haynaldia villosa ditelosomic addition line Pana (highly resistant to the powdery mildew). The seed-setting rate, agronomic characters, genomic in situ hybridization (GISH) of hybrid progenies，and resistances to stripe rust and powdery mildew were investigated. The results showed that the seed-setting rate of hybridization via Moisson 6Mv/6B as female donor (80.56%) was significant higher than that via Pana as female donor (58.33%). The seed-setting rate was associated with the hybrid methods (χ2 = 4.96> χ20.05 = 3.84 (df = 1)). The plant height of hybrid progenies was about 10 cm higher than Pana, the parent with maximum height. And the maturity of hybrid progenies was about two weeks earlier than that of the parents. In the hybrid progenies, the plants with the 6Mv chromosome have the resistance to stripe rust and the plants with the telosome from Haynaldia villosa have the resistance to powdery mildew. It was found that four plants with both the 6Mv chromosome and the telosome from Haynaldia villosa were resistant to stripe rust and powdery mildew. It indicated that the resistance to stripe rust and powdery mildew aggregated, and no mutual inhibition was found. It implied that the aggregation of the two resistance genes was able to provide the new wheat germplasm with the resistances to stripe rust and powdery mildew. 3. Moisson 6Mv/6B was irradiated with 60Co-γ rays of 6Gy (120rad/min) during meiosis. The agronomic characters and chromosomes confirmation in the mitotic metaphase of root tip cells，as well as resistance to stripe rust were investigated. The seed-setting rate of irradiated plants was only 2.22%. The chromosomes in mitotic metaphase had clear fragments. The resistance to stripe rust of progeny of irradiated plants was the adult-plant resistance. The wheat- Aegilops ventricosa 6Mv/6B substitution line is a good stripe rust resistance donor for its stabile resistance. Our study demonstrated that the key for use the resistance is to choose a good receptor. There is no difference between Moisson 6Mv/6B be the female and be the male if the seed number meets the requirement. At the same time, the stripe rust resistance of Moisson 6Mv/6B did not have the mutual inhibition with the powdery mildew resistance from Haynaldia villosa. It is able to aggregate the two resistances for wheat breeding.

西部大开发中退耕还林(草)面临的挑战及其作用

根据中国西部森林的现状 ,采用生态学与地理学的方法 ,分析了因毁林开荒造成水土流失、洪涝灾害、沙尘风暴、干旱少雨、江河断流、物种减少等危害对国民经济发展所造成的影响 ,以及近几年所产生的一系列生态环境问题 .探讨了退耕还林 (草 )对我国的生态环境治理、西部持续发展、江河整治、国土整治综合措施运用、西部农民脱贫致富的作用 .

中国水土流失的成因、威胁及其防治途径

本文在对以往研究结果进行综合分析的基础上 ,提出了我国水土流失的成因、危害及防治途径。

中国土壤侵蚀影响因素及其危害分析

该文从降雨、地貌、植被、土壤及人为因素等方面对中国土壤侵蚀的影响因素进行了系统分析 ,研究了土壤侵蚀使中国土地资源破坏、粮食减产、灾害发生、土壤肥力下降、水资源环境污染、库塘湖泊淤积、城市安全受到威胁等一系列危害 ,可供水土保持及环境整治决策作参考

中国水土流失的基本概况及其综合治理

水土流失是一个全球性的环境问题，给人类的生命与物质财产造成极大的危害与破坏，在现代社会，由于人口的持续增长，社会经济生产规模的不断扩大，植被破坏的急剧发展，对国家经济的发展构成威胁．在上述背景下．根据“再造—个山川秀美的西北地区”之要求，我国水土流失的防治必须因地制宜，坚持综合治理．