Epigenetic marks in cloned rhesus monkey embryos: Comparison with counterparts produced in vitro

Until now, no primate animals have been successfully cloned to birth with somatic cell nuclear transfer (SCNT) procedures, and little is known about the molecular events that occurred in the reconstructed embryos during preimplantation development. In man

Single base-resolution methylome of the silkworm reveals a sparse epigenomic map

Epigenetic regulation in insects may have effects on diverse biological processes. Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). We conservatively estimate that 0.11% of genomic cytosines are methylcytosines, all of which probably occur in CG dinucleotides. CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. We find that transposable elements, promoters and ribosomal DNAs are hypomethylated, but in contrast, genomic loci matching small RNAs in gene bodies are densely methylated. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis(1) and human(2), demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.

猕猴体细胞核移植影响因素的研究：着床前胚胎表观遗传重编程和供体细胞周期同步化

体细胞核移植（somatic cell nuclear transfer）克隆技术的成功，特别 是运用终末分化的淋巴细胞和嗅觉神经元细胞成功克隆出小鼠，证实了分化的体 细胞核潜在的发育全能性。该技术已经在多个物种上成功地得到克隆后代，在转 基因动物、基因敲除动物和疾病模型动物生产中也得到成功应用，在结合干细胞 技术的治疗性克隆和再生医学方面也取得了初步成果，展现出了具有深远意义的 应用前景。但是，目前该领域仍然存在着很多急待解决的重要问题：克隆成功率 低，克隆胚和克隆动物经常呈现发育异常，妊娠和出生前后的高死亡率。对哺乳 动物早期胚胎发育过程中DNA 甲基化、组蛋白修饰等表观遗传重编程 （epigenetic reprogramming）机制的深入了解，有助于研究体细胞核在去核卵 母细胞中的表观遗传重编程事件，进而改善克隆胚重编程效率和发育能力。 猕猴是一种重要的实验动物，在人类疾病模型和生物医药研究中有重要的意 义。本研究主要围绕猕猴体细胞克隆胚胎早期发育过程中的表观遗传重编程事件 和核移植前体细胞同步化处理这两方面展开。1），首次详细地了描绘了猕猴着床 前胚胎发育过程中整体水平的DNA 甲基化表观遗传重编程事件，研究发现在受精 卵中父本基因组形成原核后迅速地发生了去甲基化，在2 细胞期后的卵裂过程 中，母本基因组才开始逐渐地去甲基化，到桑葚胚达到最低水平，然后开始重新 （de novo）甲基化,到囊胚期时形成不对称的甲基化模式，滋养外胚层（TE）呈 现高甲基化状态，而内细胞团（ICM）呈现低甲基化状态,这一不对称模式可能是 灵长类动物特有的，其他哺乳动物呈现正好相反的不对称模式。2），研究发现， 大多数猕猴克隆胚胎的DNA 甲基化重编程存在异常,效率低。很多2 细胞期克隆 胚（67%）和8 细胞期克隆胚（50%）的核DNA 甲基化水平显著高于对应的体 外受精胚，8 细胞克隆胚之间呈现多种不同的表观遗传特征。大多数克隆囊胚的 ICM 细胞核的甲基化水平显著高于IVF 囊胚，这些异常可能是导致克隆胚胎移 植到代孕母体后发育时间不长就失败的原因。3），在核移植前对猕猴成纤维细 胞同步化处理的研究中发现，血清饥饿，细胞周期阻断剂DMSO（二甲基亚砜）、 roscovitine、aphidicolin 和indirubin 的处理都有显著的同步化效果，提高了G0+G1 期细胞的比例。经过BrdU 标记法证实了这几种处理方法抑制细胞增殖的效果，并且证实了这种周期阻滞作用是可逆的。用原位末端标记法（TUNEL）分析证 实，血清饥饿1 到4 天后细胞凋亡比例显著上升，在贴壁的细胞中约有6%发生 凋亡，而正常对照只有1%左右，而周期阻断剂处理没有增加细胞凋亡率，这提 示这些周期阻断剂可能是一种相对安全且有效的猕猴成纤维细胞处理方法。核移 植前对猕猴成纤维细胞进行处理，有助于优化体细胞核移植技术，也是改善体细 胞核在克隆胚中重编程效率的重要途径。

海拉尔盆地贝尔凹陷基岩潜山特征及储层预测

The lithology of the buried hill of Triassic Budate group in Beier depression is epimetamorphic clastic rock and volcanic clastic rock stratum. Recently the favorable hydrocarbon show was discovered in buried hill of base rock, and large-duty industrial oil stream was obtained in some wells in Beier depression. Based on the information of seismos and wells, the tectonic framework, tectonic deformation times and faulted system of the Beier depression are comprehensively studied, then configuration, evolutional history, genetic type and distributed regularity of buried hill are defined. According to observing description and analysis of core sample, well logging and interpretive result of FMI, the lithological component, diagenetic type and diagenetic sequence of buried hill reservoir are confirmed, then reservoir space system of buried hill is distinguished, and vegetal feature, genetic mechanism and distributed regularity of buried hill fissure are researched, at the same time the quantitative relationship is build up between core fissures and fissures interpreted by FMI. After that fundamental supervisory action of fault is defined to the vegetal degree of fissure, and the fissure beneficial places are forecasted using fractal theory and approach. At last the beneficial areas of Budate group reservoir are forecasted by reservoir appraisal parameters optimization such as multivariate gradually regression analysis et. al. and reservoir comprehensive appraisal method such as weighing analyze and clustering procedure et. al. which can provide foundation for the next exploratory disposition. Such production and knowledge are obtained in this text as those: 1. Four structural layers and two faulting systems are developed, and four structural layers are carved up by three bed succession boundary surfaces which creates three tectonic distortional times homology. Three types of buried hill are divided, they are ancient physiognomy buried hill, epigenetic buried hill, and contemporaneous buried hill. 2. Reservoir space of Budate buried hill is mainly secondary pore space and fissure, which distributes near the unconformity and/or inside buried hill in sections. The buried hill reservoir experienced multi-type and multi-stage diagenetic reconstruction, which led to the original porosity disappeared, and multi secondary porosity was created by dissolution, superficial clastation and cataclasis et. al. in diagenetic stage, which including middle crystal pore, inter crystal pore, moldic pore, inter particle emposieu, corrosion pore space and fissure et. al. which improved distinctly reservoir capability of buried hill. 3. The inner reservoir of buried hill in Beier depression is not stratigraphic bedded construction, but is fissure developing place formed by inner fault and broken lithogenetic belt. The fissures in inner reservoir of buried hill are developed unequally with many fissure types, which mainly are high angle fissure and dictyonal fissures and its developing degree and distribution is chiefly controlled by faulting. 4. The results of reservoir comprehensive evaluate and reservoir predicting indicates that advantageous areas of reservoir of buried hill chiefly distributes in Sudeerte, Beixi and Huoduomoer, which comprehensive evaluate mainly Ⅱand Ⅲ type reservoir. The clues and results of this text have directive significance for understanding the hydrocarbon reservoir condition of buried hill in Beier depression, for studying hydrocarbon accumulated mechanism and distributed regularity, and for guiding oil and gas exploration. The results of this text also can enrich and improve nonmarine hydrocarbon accumulated theory.

火成岩储层裂缝识别、预测理论与方法研究－以济阳坳陷火成岩油藏为例

Since 1970s, igneous reservoirs such as Shang741, Bin674 and Luol51 have been found in Jiyang depression, which are enrichment and heavy-producing. Showing good prospect of exploration and development, igneous reservoirs have been the main part of increasing reserves and production in Shengli oilfield. As fracture igneous reservoir being an extraordinary complex concealed reservoir and showing heavy heterogeneity in spatial distribution, the study of recognition, prediction, formation mechanism and the law of distribution of fracture is essential to develop the reservoir. Guided by multiple discipline theory such as sedimentology, geophysics, mineralogy, petroleum geology, structural geology and reservoir engineering, a set of theories and methods of recognition and prediction of fractured igneous rock reservoir are formed in this paper. Rock data, three-dimensional seismic data, log data, borehole log data, testing data and production data are combined in these methods by the means of computer. Based on the research of igneous rock petrography and reservoir formation mechanism, emphasized on the assessment and forecast of igneous rock reservoir, aimed at establishing a nonhomogeneity quantification model of fractured igneous rock reservoir, the creativity on the fracture recognition, prediction and formation mechanism are achieved. The research result is applied to Jiyang depression, suggestion of exploration and development for fractured igneous rock reservoir is supplied and some great achievement and favourable economic effect are achieved. The main achievements are gained as follows: 1. The main facies models of igneous rock reservoir in JiYang depression are summarized. Based on data and techniques of seism, well log and logging,started from the research of single well rock facies, proceeded by seismic and log facies research, from point to line and line to face, the regional igneous facies models are established. And hypabyssal intrusion allgovite facies model, explosion volcaniclastic rock facies model and overfall basaltic rocks facies model are the main facies models of igneous rock reservoir in JiYang depression. 2. Four nonhomogenous reservoir models of igneous reservoirs are established, which is the base of fracture prediction and recognition. According to characteristics of igneous petrology and spatial types of reservoir, igneous reservoirs of Jiyang depression are divided into four categories: fractured irruptive rock reservoir, fracture-pore thermocontact metamorphic rock and irruptive rock compound reservoir, pore volcanic debris cone reservoir and fracture-pore overfall basaltic rock reservoir. The spatial distribution of each model's reservoir has its features. And reservoirs can be divided into primary ones and secondary ones, whose mechanism of formation and laws of distribution are studied in this paper. 3. Eight geologic factors which dominate igneous reservoirs are presented. The eight geologic factors which dominates igneous reservoirs are igneous facies, epigenetic tectonics deformation, fracture motion, intensity of intrusive effect and adjoining-rock characters, thermo-contact metamorphic rock facies, specific volcano-tectonic position, magmatic cyclicity and epigenetic diagenetic evolution. The interaction of the eight factors forms the four types nonhomogenous reservoir models of igneous reservoirs in Jiyang depression. And igneous facies and fracture motion are the most important and primary factors. 4. Identification patterns of seismic, well log and logging facies of igneous rocks are established. Igneous rocks of Jiyang depression show typical reflecting features on seismic profile. Tabular reflection seismic facies, arc reflection seismic facies and hummocky or mushroom reflection seismic facies are the three main facies. Logging response features of basic basalt and diabase are shown as typical "three low and two high", which means low natural gamma value, low interval transit-time, low neutron porosity, high resistivity and high density. Volcaniclastic rocks show "two high and three low"-high neutron porosity, high interval transit-time, low density, low-resistance and low natural gamma value. Thermo-contact metamorphic rocks surrounding to diabase show "four high and two low" on log data, which is high natural gamma value, high self-potential anomaly, high neutron porosity, high interval transit-time and low density and low-resistance. Based on seismic, well log and logging data, spatial shape of Shang 741 igneous rock is described. 5. The methods of fracture prediction and recognition for fractured igneous reservoir are summarized. Adopting FMI image log and nuclear magnetic resonance log to quantitative analysis of fractured igneous reservoir and according to formation mechanism and shape of fracture, various fractures are recognized, such as high-angle fracture, low-angle fracture, vertical fracture, reticulated fracture, induced fracture, infilling fracture and corrosion vug. Shang 741 intrusive rock reservoir can be divided into pore-vug compound type, pore fracture type, micro-pore and micro-fracture type. Physical properties parameters of the reservoir are computed and single-well fracture model and reservoir parameters model are established. 6. Various comprehensive methods of fracture prediction and recognition for fractured igneous reservoir are put forward. Adopting three-element (igneous facies, fracture motion and rock bending) geologic comprehensive reservoir evaluation technique and deep-shallow unconventional laterolog constrained inversion technique, lateral prediction of fractured reservoir such as Shang 741 is taken and nonhomogeneity quantification models of reservoirs are established.

内陆湖沼相和近海沼泽相沉积环境煤中微量元素和矿物的对比研究—以辽宁北票、江西建新和桥头煤研究为例

Using knowledge of geology, geochemistry, coal petrology, mineralogy, by means of a variety of advanced measuring methods such as inductively coupled plasma mass spectrometry (ICP-MS), inductively coupled atomic emission spectrometry (ICP-AES), X-ray powder diffraction (XRD), scanning electron microscopy with energy-dispersive spectrometer(SEM-EDS), sequential chemical extract and density fractions, the characteristics of trace elements and minerals in Jurassic Beipiao coal mine under inland limnetic sedimentary environment and in late Permian Jianxin and Qiaotou coal mines under paralic swamp sedimentary environment were studied. Compared with the average concentration in the world bituminous coals, the Beipiao coal was characterized by relatively high contents of Sc, Ti, Cr, Co, Ni, Zn, Se, Sr, Zr, Y, Ba, REE and Th, and lower contents of V, Rb, Cd, Sn, Pb, Bi and U; while the Jianxin coal was relatively enriched in Li, Sc, Ga, Sr, Y, Nb, Sb, Th and U, with low concentration of Be, Co, Ni, Cu, Ge, Zr, Mo, Cd, Cs, Ba, Pb and Bi; and the Qiaotou coal was enriched in Li, Sc, Sr, Nb, Ta, Zr, REE, Hf, Th and U, with low concentration of Be, V, Co, Ni, Cu, Ge, Mo, Cd, Cs, Ba, Tl, Pb and Bi. The concentrations of Ca, Mg and K in Beipiao coal are higher than those in Jianxin coal and Qiaotou coal, while Fe, S and Ti in Beipiao coal are lower than those in Jianxin coal and Qiaotou coal. The proximate analysis of coal samples was carried out, which indicated that Beipiao coal was medium- to high- ash (5.92-60.68%) with low sulphur coal, and Jianxin coal and Qiaotou coal was medium to high ash (8.85-46.33%) with high sulphur. The reflectivity was measured, which explained that Beipiao coal belonged to high volatile bituminous coal, Jianxin coal was low volatile bituminous coal and Qiaotou coal was low volatile anthracite. Quantitative maceral analyses were studied. The characteristics of rare earth elements (REE) were investigated, which showed that the total contents of REE were higher than that of the world's average content. With the increase of coal's metamorphic grade, the total contents of REE decreased from 98.5 X 10"6 of Beipiao coal to 94.2 X 10"6 of Jianxin coal, and to 75.9 X 10"6 of Qiaotou coal, and 5Eu reduced which indicated that the element Eu depleted. The characteristics of REE was controlled by the metamorphic grade of coal. And REE were mainly absorbed in clay minerals in Beipiao coal samples, while in Jianxin and Qiaotou coal mines, REE were primarily related to clay mineral and pyrite. The variation of trace elements in vertical direction of coal seams was studied, and the results showed that different trace elements differed greatly. The correlation between trace elements and ash were determined. Four major trace elements (aluminium-silicates, sulphide, carbonate and phosphate) accounted for the occurrence and distribution of most elements studied were determined. Coal samples were separated by density fraction, which showed that Cr, Cu, Mo and Pb were closely related to inorganic matters mainly distributed in P >2.6 and dropped remarkably in the density fractions P <2.3 . The occurrences of Co, Cr, Ni, As, Se, Mo, U were studied directly and quantitatively using sequential chemical extract with six steps, which showed that Co. Ni, Mo and U were mainly in the form of mineral, and As, Se chiefly in the form of organic state, while Cr mostly in the form of organic state and mineral. Major mineral phases presented in the Beipiao coal were Kaolinite, illite, quartz, calcite, and small amount of siderite, barite. While major mineral phases in Jianxin and Qiaotou coal were pyrite, kaolinite, and small amount of marcasite, rutile, sphalerite. This is the first time that the chromite in the coal was discovered in China, which indicates that Cr occurrence appeared in the form of chromite. The ratio of Sr/Ba, Sr/Ca and V/Ni in Beipiao coal mine under inland limnetic is smaller than that of in Jianxin and Qiaotou coal mines under paralic swamp. The ratio of K/Na and Th/U of Beipiao coal mine is higher than that of Jianxin and Qiaotou coal mine, which proved that Beipiao coal was not affected by sea water and Jianxin and Qiaotou coal were affected by sea water. Trace elements such as Cr, Ni, Mo in minerals were analyzed by SEM-EDS. The factors controlling the enrichment of trace elements can be divided into syngenetic stage factors and epigenetic stage factors.

A preliminary discussion on the bio-metallogenesis of Tl deposits in the low-temperature minerogenetic province of southwestern China

Starting with the research status of bio-metallogenesis of Tl deposits and their geology, this work deals with the geological background of Tl enrichment and mineralization and the mechanism of bio- metal-logenesis of Tl deposits, as exemplified by Tl deposits in the low-temperature minerogenetic province. This research on the bio-metallogenesis of Tl deposits is focused on the correlations between bio-enrichment and Tl, the enrichment of Tl in micro-paleo-animals in rocks and ores, bio-fossil casts in Tl-rich ores, the involvement of bio-sulfur in minerogenesis and the enrichment of bio-genetic organic carbon in Tl ores. Thallium deposits have experienced two ore-forming stages: syngenetic bio- en-richment and epigenetic hydrothermal reworking (or transformation). Owing to the intense epigenetic hydrothermal reworking, almost no bio-residues remain in syngenetically bio-enriched Tl ores, thereby the Tl deposits display the characteristics of hydrothermally reoworked deposits.