配液结晶法制备溶菌酶蛋白质晶体的生长机理研究

采用配液结晶法制取了溶菌酶蛋白质晶体,使用动态光散射测量了溶液中聚集体的颗粒度几率分布;使用Zeiss显微镜测定了溶菌酶(110)晶面的生长速度.实验表明:随着蛋白质和NaCl浓度的增加,溶液中聚集体的颗粒尺寸也相应增加.随着反应时间的增加,溶菌酶分子在溶液中的聚集反应,逐渐达到平衡;在蛋白质和NaCl浓度较高时,溶菌酶晶体的(110)面生长较快,而在蛋白质和NaCl浓度较低时,该晶面生长较慢.基于二维成核生长机理,从晶体生长动力学理论方程出发,计算了二维成核的形成能a=4.01×10-8J?cm-2.

Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method

The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaCl, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other. The higher the concentrations of lysozyme and NaCl were, the faster the growth rate of (I 10) face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy (a) of growing steps were calculated about 4.01 X 10(-8) J.cm(-2) according to the model of multiple two-dimensional nucleation mechanism.

超重环境对斑马鱼仔鱼早期发育和耳石形态学与化学组成的影响

目的本研究以模式动物斑马鱼为研究对象,通过研究超重下鱼体的早期发育和耳石变化,探讨了脊椎动物前庭系统对超重环境的适应机制。方法斑马鱼受精卵在受精后10h分别置于1G和5G重力环境中,观察鱼体发育状态和耳石的形态;使用Zeiss软件比较1G和5G下耳石大小;使用EDAX检测耳石微化学成分含量变化。结果1)斑马鱼从鱼卵向早期仔鱼发育过程中超重环境影响仔鱼发育,耳石形态在初期显著变大(P<0.01),其后耳石变小;2)EDAX检测表明,耳石中S和Sr含量升高,Na和Ca的含量减少;3)出现矢耳石与微耳石融合的现