Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus

A putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved.

COMPARISONS OF PHASE TIMES WITH TUNNELING TIMES BASED ON ABSORPTION PROBABILITIES

The times spent by an electron in a scattering event or tunnelling through a potential barrier are investigated using a method based on the absorption probabilities. The reflection and transmission times derived from this method are equal to the local Larmor times if the transmission and reflection probability amplitudes are complex analytic functions of the complex potential. The numerical results show that they coincide with the phase times except as the incident electron energy approaches zero or when the transmission probability is too small. If the imaginary potential covers the whole space the tunnelling times are again equal to the phase times. The results show that the tunnelling times based on absorption probabilities are the best of the various candidates.

Seismic characteristics of a reef carbonate reservoir and implications for hydrocarbon exploration in deepwater of the Qiongdongnan Basin, northern South China Sea

Our analysis of approximately 40,000 km of multichannel 2-D seismic data, reef oil-field seismic data, and data from several boreholes led to the identification of two areas of reef carbonate reservoirs in deepwater areas (water depth >= 500 in) of the Qiongdongnan Basin (QDNB), northern South China Sea. High-resolution sequence stratigraphic analysis revealed that the transgressive and highstand system tracts of the mid-Miocene Meishan Formation in the Beijiao and Ledong-Lingshui Depressions developed reef carbonates. The seismic features of the reef carbonates in these two areas include chaotic bedding, intermittent internal reflections, chaotic or blank reflections, mounded reflections, and apparent amplitude anomalies, similar to the seismic characteristics of the LH11-1 reef reservoir in the Dongsha Uplift and Island Reef of the Salawati Basin, Indonesia, which house large oil fields. The impedance values of reefs in the Beijiao and Ledong-Lingshui Depressions are 8000-9000 g/cc x m/s. Impedance sections reveal that the impedance of the LH11-1 reef reservoir in the northern South China Sea is 800010000 g/cc x m/s, whereas that of pure limestone in BD23-1-1 is > 10000 g/cc x m/s. The mid-Miocene paleogeography of the Beijiao Depression was dominated by offshore and neritic environments, with only part of the southern Beijiao uplift emergent at that time. The input of terrigenous sediments was relatively minor in this area, meaning that terrigenous source areas were insignificant in terms of the Beijiao Depression: reef carbonates were probably widely distributed throughout the depression, as with the Ledong-Lingshui Depression. The combined geological and geophysical data indicate that shelf margin atolls were well developed in the Beijiao Depression, as in the Ledong-Lingshui Depression where small-scale patch or pinnacle reefs developed. These reef carbonates are promising reservoirs, representing important targets for deepwater hydrocarbon exploration. (C) 2008 Elsevier Ltd. All rights reserved.

东营凹陷成藏组合体研究与勘探实践

With the development of petroleum exploration, subtle reservoir has become the main exploration object in Dongying Depression, which requires some new technologies and methods to further reveal the geological characteristics in step with the mature exploration stage. In this paper, on the references to the studies of petroleum system and multiple oil-gas accumulation belt with flexible maneuverability, and the application of systematic theory, the concept of reservoir assemblage is initially defined as "the association of active source rock(s) and hydrocarbon reservoir(s) that are genetically related, with the bridge of pathway system in an oil and gas bearing basin". Compared with the theories of petroleum system and multiple oil-gas accumulation belts, it emphasizes on the processes of petroleum migration and accumulation and the correlation among active source rock, trapped hydrocarbon and migration pathway, and has been confirmed to be more suitably applied to high maturely explored basin. In the first study of this paper, sequence stratigraphy and subtle analytical technology of source rock have been employed to find that two categories of source rock with their characteristic types of organic matter and substantial states occurred in Dongying Depression. The first category, consisting of the oil shales within the third-order sequences of lacustrine expanding system tracts in the upper interval of the fourth Member of Shahejie Formation and both in the middle and lower intervals of the third Member of Shahejie Formation, is featured with the highest abundance of total organic matter (TOC) and the strongest abilities of hydrocarbon generation and expulsion, which is classified into the standard of good hydrocarbon source rock. Exploration assessment confirmed that about 70-80% of hydrocarbon in Dongying Depression came from this set of source rock for which the low sedimentary rate and strong oxygen-free environment would play the key role during its generation. The second category, composed of organic matter of dark mudstone in high stand system tracts in the upper and middle intervals of the third Member of Shahejie Formation, has been characterized by low content of total organic matter which mostly dispersedly distributes, and formed in the pre-delta to delta front environments. In classification, it belongs to the ordinary standard of source rocks. In the second research part, through the studies of high frequency sequence stratigraphy, fault geometry and active history combining with geochemistry of fluid inclusion and nitrogen compound and simulation test of hydrocarbon migration and accumulation, the faults have been thought to be the principal conduits, and the sandy bodies and unconformities might played the complementary pathways for hydrocarbon migration and accumulation in Dongying Depression of the continental faulted basin. Therefore, the fault activities may mainly constrain on the development of hydrocarbon pathways in space and time. Even more, using homogenization temperatures of fluid inclusion in digenetic minerals, three critical moments for hydrocarbon accumulation have been determined as well in Dongying Depression, which happened during the late stage of Dongying Formation (Ed), the early stage of Guantao Formation (Nig) and the early stage of Minghuazhen Formation (Nim), respectively. Comparatively, the last stage is looked as the main forming-reservoir period, which has also been supported by the results of geochemical analysis and simulation experiments of hydrocarbon generation and expulsion. Clearly, the times of hydrocarbon migration and accumulation are consistent with those of the fault activities in Dongying Depression, which indicate that tectonic activities would control the forming-reservoir. A conceptual model of faulting-episodic expulsion coupled with episodic forming-reservoir has then been established in this study. In the third part of this paper, some focusing areas were selected for the fine descriptions of pathway distribution and forming-reservoir, which has given four types of reservoir assemblage in terms of the main pathway and its correlation with the reservoir and trap: (1) mainly consisted of sandy bodies; (2) mainly consisted of faults; (3) mainly consisted of unconformities; and (4) their complex with two or three types of pathways. This classified criteria has also been applied to access the risk of some prospected traps in Dongying Depression. Finally, through the application of reservoir assemblage integrated with pathway distribution to all the prospective targets in Dongying Depression, the new favorably hydrocarbon accumulated belts have been figured out, and more subtle reservoirs have also been found. For examples, during 2000 and 2002, in the mature exploration areas, such as Liangjialou and Shengtuo structural closures etc., newly proved reserves were 2274 * 104t, and forecasted oil reserves 5660-5860xl04t; and in the predicted favorable areas, newly additional controlled oil reserves was 3355xl04t. Besides those, many other favorable exploration areas need to be further appraised.

小鲵属分子系统发育研究

小鲵属为亚洲特有的有尾两栖类，是小鲵科之模式属。现记载小鲵属动物有29种，占全科物种数一半以上（Frost, 2007），为小鲵科第一大属。该属分布跨越古北界和东洋界，分布于中国、朝鲜、韩国、日本等地区，其系统学研究一直以来颇为中外学者所关注。澄清该属的物种分类问题，阐明其种间的系统发育关系对整个小鲵科的系统演化与分布格局关系的研究具有关键性意义。 本论文以中国及周边地区的小鲵属物种为主要对象，主要利用分子生物学实验与生物信息学途径相结合的手段，运用支序系统学与分子进化生物学理论及分析方法，展开系统发育的研究。在此基础上诠释现存的分类问题，并探讨该属系统发育关系。 研究材料上，本研究采用野外采集与网络下载数据相结合的方法，获取了较为全面的小鲵属物种DNA序列资料。技术手段上，选取了线粒体DNA的Cytb、12S、16S、NADH 2、COI等多个基因部分片段序列，对小鲵属开展了较为全面系统的研究。分析方法上，针对小鲵属物种各类群的具体情况，运用了处于领域前沿的多种分析方法。应用PAUP、MrBayes、Modeltest、Mega等软件，采用了最大简约法（MP）、邻接法（NJ）、贝叶斯推断（BI）及K2P遗传距离分析等方法。 本研究对小鲵属进行了较为全面的系统发育研究，弥补了有关小鲵属系统发育研究的不足，并得出了以下结果： （1）关于豫南小鲵Hynobius yunanicus的有效性，基于细胞色素b序列的系统发育关系联合形态和染色体组型等证据证明了豫南小鲵是商城肥鲵的同物异名。 （2）获得了较为全面的小鲵属物种系统发育树，并以此解释了北海道滞育小鲵、东北小鲵、中国小鲵与义乌小鲵等存在的分类问题。 （3）本研究利用DNA条形码技术对小鲵属及小鲵科物种进行了鉴定，再次证明豫南小鲵为商城肥鲵的同物异名；并认为猫儿山小鲵与挂榜山小鲵为同物异名。 综上，本研究较为完整地勾勒了小鲵属的系统发育关系全貌，并对小鲵属物种的起源进行了推测。 Hynobius, the type genus of the Family Hynobiidae, is the only exclusively Asian salamander genus. This genus which contains 29 species (beyond half of total Family), is the key group in Hynobiidae. The genus distributed across Palaearctic and Oriental Realm, and was found in China, Korea, and Japan. Systematics of genus Hynobius draws attention of researchers all the times. Resolving the taxonomic and phynogenetic questions of Hynobius is very important to the evolutionary research of Family Hynobiidae. Firstly, studies on systematics of genus Hynobius based on morphology, karyotype and molecular phylogeny of Hynobius are reviewed along with existing questions of this genus. The sequential reaserch project of phylogenetics is perspectively outlined. Using molecular data, we compared Hynobius yunanicus with a sympatric species Pachyhynobius shangchengensis. Our cytb sequences associating with karyotypic and morphological data supportted that H. yunanicus is not a valid species, but a synonym of P. shangchengensis. Because of phenotypic plasticity, some morphological characters are not even suitable for identifying hynobiids. The taxonomy of hynobiids is still controversial to a certain extent (Zhao et al. 1993; Fei, 1999; Chen et al. 2001; Zeng et al. 2006) and needs to be resolved by a new method. Here we examined the utility of COI barcoding for the discrimination of hynobiids. Meantime, the taxonomy of this Family was looked-over again. Our result show that the DNA Barcoding based on COI is easier and more rapidly than classic methods. And the DNA Barcodes data supported the actual taxonomy of Hynobiidae. Based on the achievements of our research, the phylogeny of Hynobius was reconstructed including some new species (H. maoershanensis, H. guabangshanensis, etc). Besides the phylogenetics of Hynobius was outlined, some questions and the hypothesis about the origin of genus Hynobius was put out.

STL格式文件拓扑重建及快速切片算法研究

建立三角面片之间拓扑关系是提高分层切片效率的关键因素之一,提出基于标准模板库set容器的拓扑关系重建算法及快速切片算法,有效地去除了STL文件大量冗余数据,简化了数据的存储,该算法每一个三角面片在每一切平面内只求一次交点,切片完成后可直接得到封闭轮廓环,以减少在切片过程中对三角面片的遍历次数、排序次数及求交点计算量,简化了轮廓环的构造过程,从而有效地提高了算法的效率。

准噶尔盆地滴南凸起中生界层序地层及有利勘探目标优选

Based on the theories of sequence stratigraphy and sedimentology, as well as comprehensive studies of seismic data, drilling data, core interpretation and setting of this area, the thesis presents an analysis for Mesozoic formation in Dinan uplift. By means of recognizing the boundary of the sequence, dividing and correlating the systems tract, Mesozoic of Dinan uplift is divided into ten sequences and twenty-five systems tracts during the establishment of the sequence framework. In the framework, some sequences are featured by mature systems of lowstand, water-transgression and highstand, while some undeveloped systems of lowstand or highstand. The main sedimentary facies in Mesozoic of Dinan uplift are braided river, meandering river, delta and lake. The braided river was divided into sandy river and rudaceous river by the lithology of the river channel and was divided into dry climate and wet climate condition by the color of the flood plain. Additionally, The concept of “wetland” is put forward for the first time and regarded as the consequence of wet climate. The analysis includes the classification of six types of traps: (1) stratigraphic overlap trap, (2) lithologic trap with updip pinchout, (3) stratigraphic unconformity trap, (4) fault-lithology trap, (5) fault trap, (6) anticlinal trap, and combining with the research of the characteristics and distribution rules for the known reservoir, it draws out that “fault control” is the petroleum accumulation pattern in this area, in which fault is the key element of the transporting system. Finally the thesis concludes the distribution characteristics and optimized some targets for the potential exploration zone.

磁层-电离层耦合现象的分析研究

The magnetosphere-ionosphere coupling is mainly manifested by the trans- porting processes of energy into the ionosphere , the energy is carried by solar wind and firstly accumulate at the magnetosphere, and the coupling processes also significantly include the interaction between the magnetosphere and ionosphere for mass and energy. At the quiet condition, energy is delivered by the large-scale convection of the geomagnetic field; the huge energy from solar wind bulk will be injected into and consumed at the near magnetosphere and ionosphere by the geomagnetic storm and substorm activities. Aurorae and FACs (Field-aligned currents) are the important phenomena in the coupling processes. In the present work, firstly, we analyze the activity characteristics of auroral precipitating particle, secondly, we study the distribution characters of large-scale field aligned currents (LS FACs) at storm-time using the observations from different satellites at different altitudes. Finally, we investigate the evolution of the geomagnetic field configuration at the nightside sector on the onset of the expansion phase in a substorm event, the substorm event happened at 0430UT to 0630UT on 8th Nov. 2004. The main results as follows： At the first, the data of the estimated power input (EPI) of auroral particles from NOAA/POES (Polar orbiting environmental satellite) for some 30 years have been analyzed. The variation tendencies of the EPI generally coincide with aa, AE and Dst indices. The annual variation of EPI shows equinox peaks and an asymmetric-activity with a higher peak in the winter-hemisphere than in the summer-hemisphere. The diurnal UT variations are different from north and south hemisphere: for north hemisphere, the peak appears at 1200UT, and the relative deviation is 22% to the daily average of the north hemisphere. For south hemisphere, the maximal deviation is 22% at 2000UT. So the diurnal variation of EPI is more dominant than the annual variation which maximal deviation is 3% to 12% for different seasons. Studies on correlations of the hourly average of EPI, Pa, with AE and Dst indices show a correlation coefficient r=0.74 of Pa and AE, and r=-0.55 of Pa and Dst. The hourly EPIs for north and south polar regions, NPa and SPa, show a north-south asymmetry with a higher correlation of SPa and AE (or Dst). Time delays of EPI with respect to magnetic indices are examined, the maximum correlation coefficient of Pa with AE (r=0.78) occurs when the time delay =0, suggesting a synchronous activity of auroral electrojet and auroral precipitating particles, while =1-2h, the correlation coefficient of Pa with Dst is maximum (r=0.57), suggesting that the activity of auroral particle precipitating may influence the ring current on some extent. Sencondly, we use the high-resolution magnetic field vector data of the CHAMP satellite to investigate the distribution of large-scale FACs during the great magnetic storm on 7th to 8th Nov. 2004. The results show that, whether in the northern or southern hemisphere, the number and density of large-scale FACs during the main-phase are more and bigger than these during the recover-phase, and the number of large-scale FACs in morning sector obviously is more than that in afternoon sector. In terms of the magnetic indices, we find that large-scale FACs in morning sector significantly affected by the substorm activities, while in afternoon sector the large-scale FACs mainly indicate the fluctuations of the ring-current in storm time. Accordingly to the former studies, similarly, we find that in the morning sector, the scale of the large-scale FACs move to the high-latitude region, and in the afternoon sector, large-scale FACs distinctly expand to the low-latitude region. During the time periods that the NOAA/POES auroral precipitating particle power data temporally correspond to the large-scale FACs, the more the power of auroral particle is, the more and bigger the number and density of FACs are. At the same time, we use the magnetic field vector data of POLAR obtain a good form of region 1, region 2, and three pieces of cusp FACs during a single transit at 1930UT-2006UT on 07th. And the characteristics of simultaneous electric field and energy particles observations on Polar are coincide with the five FACs pieces. Finally, by means of the observation of Cluster 4 and Goes 10、 Goes 12, we analyze the evolution process of the change of the magnetic field configuration at night sector at the expansion phase of a substorm event which happened during 0430UT to 0630UT on 8th Nov. 2004, we find that the times of the beginning of the polarizations of magnetic field are observed from Goes 10 to Goes 12 then to Cluster 4. So, at the synchronous orbit ( 6.6 RE) to 10RE distance scale of the neutral sheet, the current disruption spread tailward. Simultaneously, the strengthen of the FACs deduced from these satellites’ magnetic field observations are almost consistent with the times of polarizations, as well as the high energy particles injection and the electric field dominant variation. The onset times determined by the magnetic field polarizations from these satellites are all ahead of the onset time that confirmed from the auroral electrojet indices. So, these characters of different observations can be used as the criterions to determine the onset time for the substorms of such type as we studied.

准噶尔盆地西北缘三叠系层序地层及有利勘探目标选择

Based on outcrop, borehole, seismic and regional geological data, the sequence stratigraphy, sedimentary facies of the Triassic in the western margin of the Zhugaer basin was studied, and favorable exploration target was forecasted. The major achievements include: (1) the Triassic in the western margin of the Zhugaer basin can be divided into 1 second-order sequence and 5 third-order sequences, which are, in ascending order, TSQ1, TSQ2, TSQ3, TSQ4, and TSQ5. TSQ1 is equivolent to Baikouquan formation, TSQ2 is equivolent to lower Kelamayi formation, TSQ3 is equivolent to upper Kelamai formation, TSQ4 is equivolent to lower and middle Baijiantan formation, and TSQ5 is equivolent to upper Baijiantan formation. Each sequence is divided into transgressive and regressive system tracts. Thus the sequence correlation framework is established. (2) The factors controlling development of sequences are analyzed, and it is believed that tectonic is the major controlling factor. Model of sequence development is summarized. (3)Through study on sedimentary facies, 6 types of facies are recognized: alluvial fan, fan delta, braided river, braided delta, delta and lake. Their microfacies are also recognized. In this study, it is proposed that the upper and lower Kelamayi formation（TSQ2、 TSQ3）is deposited by braided river instead of alluvial fan. This conclusion is of important theoretical and practical significance.(4) The sedimentary facies map of each sequence is compiled, and the sedimentary facies developed in each sequence is determined. In TSQ1, the sedimentary facies developed is alluvial fan and fan delta. In TSQ2, the sedimentary facies developed is mainly alluvial fan and fan delta in the north, and braided river and braided delta in the south. In TSQ3, the sedimentary facies developed is mainly braided river and braided delta. In TSQ4, the sedimentary facies developed is mainly braided delta in the north, and meandering delta in the south. In TSQ5, the sedimentary facies developed is mainly braided river and braided delta. (5) In the framework of sequence stratigrahpy, favorable areas for concealed traps are forecasted, and different types of traps are developed in different system tracts. (6) Favorable areas for future exploration are predicted.

岩体力学参数及稳定性能量判据研究

There are two major problems that have been concerned all the times, which are the mechanics characters of joint rock mass and the criterion for stability of engineering rock. Aim at the two problems, several works were conducted as follow: (1) Firstly, the mechanics characters of rock mass was studied by means of the Distinct Element Code. Subsequently, it was studied that the sensibility of joint surface roughness, strength of joint wall, joint stiffness ( i.e. tangential and normal stiffness) on the rock mass strength. (2) Based on the experimental rock mass classification methods of RMR and GSI, the program of “Parameters Calculation of the Rock Mass ” was developed. It has realized the rapid choice of rock mass parameters. (3) The concept of Representive Element Volume was induced based on the study of dimensional effect of rock mass. The Representive Element Volume of the horizontal and vertical pillar (ab. Two Pillars ) in the 2nd zone of Jinchuan mine were gained by the Geology Statistic Method and the Distinct Element Code. And then, the strength and deformatiom parameters of rock mass of the Two Pillars were obtained through numerical experiment. (4) From the confining depressure after thriaxial compression test of rock sample, it was concluded that the failure of rock is caused mainly by the lateral deformation and energy release happened during the confining depressure processure. The criterion of plastic energy catastrophe of rock engineering failure was proposed and validated. Subsquently, the stability of the horizontal pillar and Qianjiangping landslide in Three Gorges was judged by means of above-mentioned method. (5) Based on the fact there is a phenomenon of increasing energy concentration while the rock mass was compressed, rock information entropy (i.e. energy distribution entropy) was proposed. And it was revealed that there was change of energy distribution entropy while the rock mass was compressed to failure.

东营凹陷下第三系岩性油气藏形成条件与预测

The Dongying depression, located in the northern part of the jiyang Sag in the Buohaiwan Basin, comprises one of the major oil-producing bases of the Shengli oil-field. The prediction and exploration of subtle or litho1ogical oil traps in the oil-field has become the major confronted target. This is also one of the frontier study areas in the highly-explored oil-bearing basins in East China and abroad. Based on the integrated analysis of the geological, seismic and logging data and the theories of sequence stratigraphy, tectono-stratigraphy and petroleum system, the paper has attempted to document the characteristics of the sequence stratigraphic and structural frameworks of the low Tertiary, the syndepositional faults and their control on deposition, and then to investigate the forming conditions and distribution of the tithological oil traps in the depression. The study has set up a set of analysis methods, which can be used to effectively analysis the sequence stratigraphy of inland basins and predict the distribution of sandstone reservoirs in the basins. The major achievements of the study are as follows: 1. The low Tertiary can be divided into 4 second-order sequences and 13 third-order sequences, and the systems tracts in the third-order sequences have been also identified based on the examination and correction of well logging data and seismic profiles. At the same time, the parasequences and their stacking pattern in the deltaic systems of the third member of the Shahejie Formation have been recognized in the key study area. It has been documented that the genetic relation of different order sequences to tectonic, climatic and sediment supply changes. The study suggested that the formation of the second-order sequences was related to multiple rifting, while the activity of the syndepositional faults controlled the stacking pattern of parasequences of the axial deltaic system in the depression. 2. A number of depositional facies have been recognized in the low Tertiary on the basis of seismic facies and well logging analysis. They include alluvial fan, fan delta or braided delta, axial delta, lowstand fan, lacustrine and gravity flow deposits. The lacustrine lowstand fan deposits are firstly recognized in the depression, and their facies architecture and distribution have been investigated. The study has shown that the lowstand fan deposits are the important sandstone reservoirs as lithological oil traps in the depression. 3. The mapping of depositional systems within sequences has revealed the time and special distrbution of depositional systems developed in the basin. It is pointed out that major elastic systems comprise the northern marginal depositional systems consisting of alluvial fan, fan delta and offshore lowstand fan deposits, the southern gentle slope elastic deposits composed of shallow lacustrine, braided delta and lowstand fan deposits and the axial deltaic systems including those from eastern and western ends of the depression. 4. The genetic relationship between the syndepositional faults and the distribution of sandstones has been studied in the paper, upper on the analysis of structural framework and syndepositional fault systems in the depression. The concept of structural slope-break has been firstly introduced into the study and the role of syndepositional faults controlling the development of sequence architecture and distribution of sandstones along the hinged and faulted margins have been widely investigated. It is suggested that structural styles of the structural slope-break controlled the distribution of lowstand fan deposits and formed a favorable zone for the formation of lithological or structure-lithological oil traps in the basin. 5. The paper has made a deep investigation into the forming condition and processes of the lithological traps in the depression, based the analysis of composition of reservoir, seal and resource rocks. It is pointed out that there were two major oil pool-forming periods, namely the end of the Dongying and Guangtao periods, and the later one is the most important. 6. The study has finally predicted a number of favorable targets for exploration of lithologieal traps in the depression. Most of them have been drilled and made great succeed with new discovered thousands tons of raw oil reserves.

东营凹陷北部陡坡带砂砾岩体性油气藏形成机理及分布规律研究

The north steep slope zone of Dongying Depression has great potential in oil resource and as the usage of 3-d seismic data in the last decade, the exploration of oil and gas has get into the stage of sandy glavel body lithological oil-gas pool exploration. In this thesis, writer take the north steep slope zone of Dongying Depression as target area and take Sha-III and Sha-[V Menber as purpose stratum, study on sequence stratigraphy, depositional system, reservoir description, emphasesing on analyzing of forming of lithological oil-gas pool, especially the dynamics principle of oil and gas preliminary movement from the source rock to the reservoir form lithological oil-gas pools. The aim of this work is to give some quantitatively explanation for the mechanism of lithological oil-gas pool forming, and set up the theory of pool form with characteristic terrestrial faulted basin. There are main conclusions and views as follow. 1. Applying with principle of sequence stratigrapgy, according to the depositional cycles of Dongying Depression, the sequence stratigraphical partition of Tertiary was finished, stressing on dismembering Sha-III and Sha-IV Menber as 5system tracts. 2. The structure of Dongying Depression especially of the north steep slope zone has accomplished, including the analyzing the structural cortroling to depositional condition of the north steep slope zone of Dongying Depression, discussed relationship between the structure of the north steep slope zone and the pool-forming. 3. The horizontal and vertical exchanges of ancient climates and ancient physiognomy of the all stratum units and studies on characteristic of depositional system distribution have been finished, found that there are five depositional systems in the north steep slope zone of Dongying Depression as fluvial, delta (tan-delta), sub-water fluvial fan lacustrine, gravitive flow, and seven formations of sandy glavel body, and forecasting of all kinds of sandy glavel body has been made. 4. Seismic stratigraphy and log stratigraphy have been made, described and forecasted all kinds of reservoir of objective stratum by means of physical geography method, setup a series means of sandy glavel body description suit to target area. 5. The pool-forming system has been studied, analyzing all the elements in petroleum sub-system of Sha-III and Sha-IV Menber of Dongying Depression with view of source controlling, estimated the petroleum system applying source rock potential index combining with distribution ofreservior. 6.Through studying types of pool, the controlling factors of pool-forming of sandy glavel body were discussed by deposition stages, formation types, structure ect. as a conclusion that the characteristics of pool forming in the north steep slope zone of Dongying Depression are, the controlling factor of the pools is mainly lithology, petrophysics of oil sands vary greatly, with a large heterogeneity, all kind of reservoir with different formation has different pool-forming conditions, and as a result, formed various pools of sandy glavel body along the steep slope with regular combination, distribution and constituted the multiple petroleum accumulative pattern. 7. It's the first time to cauculate and estimate the fluid pressure in source rock of Dongying Depression, set up the stratum fluid pressure in Dongying Depression, and firstly use equivalent charging pressure and reservoir forming index to quantitatively evaluate the pool-forming condition of lithological pool.8. Above all studies, follow up the scent of the exploration combined with practice a lot of explorative targets were found, and got geat economic and social benefit.

DAS24-3B数据采集系统控制系统软件的研制与开发

Under the auspices of the 'knowledge-Innovation Program' of CAS, Institute of Geology and Geophysics has established the Broadband Seismic Laboratory. A new kind of 24-bit high-resolution seismograph DAS24-3B has been designed and manufactured in an effort of developing China's own technology of seismic array. Since these instruments will primarily be used in field operation, there is a need to optimize the system software of data acquisition system (DAS) to enhance its stability, compatibility and maintenance. The design ideas of the system software of DAS24-3B are partly learned from the advanced DAS 72A-08. In this system there are two exclusive communication programs DNAPI-COM1 and DNAPI-LPT1, which are suitable for all standard industrial computers with ECP parallel port and serial port. By these exclusive parallel and serial communication interface the system software is split into three parts, acquisition program, user's control program and graphical display program, which can function well in separate units and can run correctly in whole. The three parts of DAS24-3B's system software possess different functions and advantages. The function of acquisition program is to control the process of seismic data acquisition. DAS24-3B system reduced its power and harddisk read-write disturbance by using the extended memory attached to its CPU, which functions as enlarging the data buffer of system and lessening the times of harddisk read-write operations. Since GPS receiver of DAS is strongly sensitive to the around environment and has the possibility of signal loss the acquisition program has been designed with the ability to automatically trail the GPS locked time. The function of user's controlling program is to configure the system's work environment, to inform the user's commands to DAS, to trail the status of DAS in real-time. The function of graphical display program is to illustrate data in figures, to convert data file into some common formatted file, to split data file in parts and combine data files into one. Both user's control program and graphical display program are API (Application Programming Interface) in window 95/98 system. Both possess the features of clearness and friendship by use of all kind of window controls, which are composed by menu, toolbar, statusbar, dialogue box, message box, edit box, scrollbar, time control, button and so on. Two programs of systemic exception handles are provided to treat the trouble in field. The DAS24-3B DAS has been designed to be easier to use-better ability, more stable and simpler. It has been tested in field and base station and has been proved more suitable for field operation of seismic array than other native instruments.

Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones

Antigen-specific circulating immunoglobulin-secreting cells (ISC) migrate to various secondary and tertiary lymphoid tissues. To understand the migration of the cells into the genital tract and its regulation by sex hormones, spleen-derived SG2 hybridoma cells secreting immunoglobulin G2b (IgG2b) and Peyer's patch-derived PA4 hybridoma cells secreting polymer IgA were labelled with (3) H-TdR, and intravenously injected into syngeneic mice of both sexes. Using flow cytometry, surface molecular markers of plasma cells, CD38 and CD138, and adhesion molecules, CD49d, CD162, and CD11a were found to be positive in SG2 and PA4 cells, but CD62L, alpha4beta7 and CD44 were not expressed on these cells. The relative distribution indexes (RDIs) of the cells in genital tract and other tissues were measured. The means of RDIs of SG2 and PA4 cells in female genital tissues were 6.5 and 4.5 times as many as the means in male genital tissues, respectively. The treatment of ovariectomized mice with beta-oestradiol significantly increased the RDIs of PA4 cells in cervix and vagina, but decreased the RDIs of SG2 cells in vagina, horn of uterus, uterus and rectum (P <0.05). Progesterone treatment increased the RDIs of PA4 cells in vagina and rectum (P <0.05). The treatment with testosterone significantly increased the RDIs of SG2 and PA4 cells in epididymis and accessory sex glands (P <0.05). These results demonstrate that the female genital tract is the preferable site for the migration of circulating hybridoma cells to the male genital tract, and sex hormones play an important role in regulation of the migration of circulating ISC to genital tracts.