Influence of Electrostatic Interaction on Fibrinogen Adsorption on Gold Studied by Imaging Ellipsometry Combined with Electrochemical Methods

Imaging ellipsometry was combined with electrochemical methods for studying electrostatic interactions of protein and solid surfaces. The potential of zero charge for gold-coated silicon wafer/solution interfaces wad determined by AC impedance method. The potential of the gold-coated silicon wafer was controlled at the potential of zero charge, and the adsorption of fibrinogen on the potential-controlled and non-controlled surfaces was measured in real time at the same time by imaging ellipsometry The effect of electrostatic interaction was studied by comparing the difference between the potential of controlled adsorption and the Potential of noncontrolled adsorption. It was shown that the rate of fibrinogen adsorption on the potentiostatic surface was faster than that on the nonpotentiostatic surface. The electrostatic influence on fibrinogen adsorption on the gold-coated silicon wafer was weak, so the hydrophobic interaction should be the major affinity.

基于RTLinux构建机载SAR实时成像处理器嵌入式控制系统

面向地学处理的机载SAR实时成像处理器由一组连接在工业总线上的数据处理板和一个嵌入式主控子系统构成 ,成像处理器的总体设计对主控系统的实时性、稳定性及可扩展性提出了严格的要求 比较了传统的嵌入式工业控制系统的设计方法 ,设计并实现一个基于廉价的PC与RTLinux的主控系统 ,分析了主控系统的两个重点 :DSP宿主环境和RTLinux下的混合任务集 ,并通过给出一个RTLinux与Linux调度精度和误差的对比试验及其结果 ,证明了基于RTLinux的主控系统对支持细粒度调度方面的优越性

海上时移地震综合解释技术研究与应用

The practical application and development of the time-lapse seismic reservoir monitor technology has indicated which has already become one of most important development technologies in seeking the surplus oil distribution and improving the reservoir recovering. The paper, first obtained the rock physics experiment analysis data according to the Bohai Sea loose sandstone in-situ measure technical, and determined the feasibility research of the S oil-field on the time-lapse seismic reservoir monitoring combining with the time-lapse numeric simulation technology, which was used to analyze the time-lapse seismic respond raw of the reservoir parameters change and pointed out the attentive problems during the real time-lapse seismic processing and interpretation. Next, simply introduced the technical link and the effect of the time-lapse mutual constrained fidelity and match processing aiming at the local complex gathering condition, geological condition, development engineering condition. Third, introduced the time-lapse integrated interpretation and the technical system with the innovative key technology that includes the time-lapse difference explanation technology, the time-lapse seismic multi-attributes integrated interpretation technology, and the time-lapse constrained reservoir parameters inversion technology, and so on. Using the time-lapse difference direct explanation technology, directly obtained the surplus oil macroscopic distribution through the difference seismic data; Using the presenting 8 big principles of the sublayer isochronisms comparison, carried on the time-lapse integrated interpretation analysis on the fine sublayer comparison and the thin oil-layer(group) contrast and the oil layer (group); The paper putted up the research, contrast, applications of the multi-sides sensitive attribute analysis and the RBF nerve network on the nearest study algorithm, and predicted the reservoir parameters and the surplus oil distribution with them; Combining with innovative researches and the time-lapse seismic constrained reservoir parameters inversion technology realized the good combination of the seismic and the reservoir engineering. Fourth, under fully analyzing the geology condition, the reservoir condition, the exploit dynamic data, and the seismic data of the S oil-field, and analyzing the time-lapse difference factors with reservoir dynamic exploit data, calibrated the oil-gas saturation change, the pressure change, the water saturation change, and determined the rationality of the time-lapse seismic difference, and finally obtained the surplus oil distribution, the water flood characteristic understanding, reservoir degasification, and pressure drop raw, and so on, which had been used in the well pattern tightening plan proof of the S oil-field development adjustment plan. Finally, the paper summarized the knowledge and understanding of the marine time-lapse seismic integrated interpretation, also had pointed out the further need researched question.

波动方程偏移速度分析

On the subject of oil and gas exploration, migration is an efficacious technique for imagining structures underground. Wave-equation migration (WEM) dominates over other migration methods in accuracy, despite of higher computational cost. However, the advantages of WEM will emerge as the progress of computer technology. WEM is sensitive to velocity model more than others. Small velocity perturbations result in grate divergence in the image pad. Currently, Kirrchhoff method is still very popular in the exploration industry for the reason of difficult to provide precise velocity model. It is very urgent to figure out a way to migration velocity modeling. This dissertation is mainly devoted to migration velocity analysis method for WEM: 1. In this dissertation, we cataloged wave equation prestack depth migration. The concept of migration is introduced. Then, the analysis is applied to different kinds of extrapolate operator to demonstrate their accuracy and applicability. We derived the DSR and SSR migration method and apply both to 2D model. 2. The output of prestack WEM is in form of common image gathers (CIGs). Angle domain common image gathers (ADCIGs) gained by wave equation are proved to be free of artifacts. They are also the most potential candidates for migration velocity analysis. We discussed how to get ADCIGs by DSR and SSR, and obtained ADCIGs before and after imaging separately. The quality of post stack image is affected by CIGs, only the focused or flattened CIGs generate the correct image. Based on wave equation migration, image could be enhanced by special measures. In this dissertation we use both prestack depth residual migration and time shift imaging condition to improve the image quality. 3. Inaccurate velocities lead to errors of imaging depth and curvature of coherent events in CIGs. The ultimate goal of migration velocity analysis (MVA) is to focus scattered event to correct depth and flatten curving event by updating velocities. The kinematic figures are implicitly presented by focus depth aberration and kinetic figure by amplitude. The initial model of Wave-equation migration velocity analysis (WEMVA) is the output of RMO velocity analysis. For integrity of MVA, we review RMO method in this dissertation. The dissertation discusses the general ideal of RMO velocity analysis for flat and dipping events and the corresponding velocity update formula. Migration velocity analysis is a very time consuming work. Respect to computational convenience, we discus how RMO works for synthetic source record migration. In some extremely situation, RMO method fails. Especially in the areas of poorly illuminated or steep structure, it is very difficult to obtain enough angle information for RMO. WEMVA based on wave extrapolate theory, which successfully overcome the drawback of ray based methods. WEMVA inverses residual velocities with residual images. Based on migration regression, we studied the linearized scattering operator and linearized residual image. The key to WEMVA is the linearized residual image. Residual image obtained by Prestack residual migration, which based on DSR is very inefficient. In this dissertation, we proposed obtaining residual migration by time shift image condition, so that, WEMVA could be implemented by SSR. It evidently reduce the computational cost for this method.

岩性油藏储层预测的地震理论与方法研究

At present the main object of the exploration and development (E&D) of oil and gas is not the structural oil-gas pools but the subtle lithological oil-gas reservoir. Since the last 90's, the ratio of this kind of pools in newly-added oil reserves is becoming larger and larger, so is the ratio in the eastern oilfields. The third oil-gas resource evaluation indicates the main exploration object of Jiyang depression is the lithological oil-gas pools in future. However, lack of effective methods that are applied to search for this kind of pool makes E&D difficult and the cost high. In view of the urgent demand of E&D, in this paper we deeply study and analyze the theory and application in which the seismic attributes are used to predict and describe lithological oil-gas reservoirs. The great results are obtained by making full use of abundant physics and reservoir information as well as the remarkable lateral continuity involved in seismic data in combination with well logging, drilling-well and geology. ①Based on a great deal of research and different geological features of Shengli oilfield, the great progresses are made some theories and methods of seismic reservoir prediction and description. Three kinds of extrapolation near well seismic wavelet methods-inverse distance interpolation, phase interpolation and pseudo well reflectivity-are improved; particularly, in sparse well area the method of getting pseudo well reflectivity is given by the application of the wavelet theory. The formulae for seismic attributes and coherent volumes are derived theoretically, and the optimal method of seismic attributes and improved algorithms of picking up coherent data volumes are put forward. The method of making sequence analysis on seismic data is put forward and derived in which the wavelet transform is used to analyze not only qualitatively but also quantitatively seismic characteristics of reservoirs.② According to geologic model and seismic forward simulation, from macro to micro, the method of pre- and post-stack data synthetic analysis and application is put forward using seismic in close combination with geology; particularly, based on making full use of post-stack seismic data, "green food"-pre-stack seismic data is as possible as utilized. ③ In this paper, the formative law and distributing characteristic of lithologic oil-gas pools of the Tertiary in Jiyang depression, the knowledge of geological geophysics and the feasibility of all sorts of seismic methods, and the applied knowledge of seismic data and the geophysical mechanism of oil-gas reservoirs are studied. Therefore a series of perfect seismic technique and software are completed that fit to E&D of different categories of lithologic oil-gas reservoirs. ④ This achievement is different from other new seismic methods that are put forward in the recent years, that is multi-wave multi-component seismic, cross hole seismic, vertical seismic, and time-lapse seismic etc. that need the reacquisition of seismic data to predict and describe the oil-gas reservoir. The method in this paper is based on the conventional 2D/3D seismic data, so the cost falls sharply. ⑤ In recent years this technique that predict and describe lithologic oil-gas reservoirs by seismic information has been applied in E&D of lithologic oil-gas reservoirs on glutenite fans in abrupt slop and turbidite fans in front of abrup slop, slump turbidite fans in front of delta, turbidite fans with channel in low slope and channel sanbody, and a encouraging geologic result has been gained. This achievement indicates that the application of seismic information is one of the most effective ways in solving the present problem of E&D. This technique is significant in the application and popularization, and positive on increasing reserves and raising production as well as stable development in Shengli oilfield. And it will be directive to E&D of some similar reservoirs

高含水油藏复合驱剩油分布研究-以羊三木油田馆陶组油藏复合驱为例

Based on the study of the combined flooding test block of Guantao formation in Third faulted block of Yangsanmu oil field, this paper carries out the integration of reservoir precise characterization for very high water cut reservoir, establishes precise 3D geologic model for high water cut development period and states the changing law of the reservoir architecture dtiring development by combined flooding. Then, by subdivided the thick oil reservoir, the study of remaining oil saturation monitoring in fiber glass cased well and tracer monitoring is developed. According the study of multiple constrained combined flooding reservoir numerical simulation, remaining oil distribution are predicted, the methods architecture of predicting remaining oil distribution are established for fluvial facies reservoir at late development stage, develops plan is designed and adjustment associating technologies for enhancing oil recovery. On these base, related measures for tapping the potential are given, it is verified and optimized through the field former test and the good economic effect is achieved . The major achievements of this paper are as follows. The changing law of the reservoir architecture and it's property parameters is revealed, The result indicates that the temperature-pressure of the injecting material and the interaction effect of the injecting material and reservoir petrography are the main factors of the dynamic changes of the reservoir architecture. The quantitative reservoir geologic model, which is tallied with dynamic reservoir parameters of the study area, is established. Subdivided the thick oil reservoir is very important for the study of the remaining oil distribution within the thick oil reservoir. Subdivided the thick oil reservoir technology, which consists of six technologies as follow: micro-cyclic divided, flow unit method, architectural element method, high resolution log technology, high resolution-process technology for normal logging data and using the production data is presented. 3. It is established dynamic monitoring system of remaining oil saturation quantitative research which are inner and interlayer remaining oil saturation from time-lapse logging in fiber glass cased well, inter-well remaining oil saturation from the technology of isotopic tracer monitoring technology, and 4d remaining oil saturation distribution from combined flooding numerical modeling integrated by production datao The forming mechanism of remaining oil for polymer flooding and alkali/polymer combined flooding is clarified, and the plane and vertical distribution law of remaining oil after combined flooding is revealed. Predicting methods and technologies for the combined flooding reservoir of fluvial facies is developed. Combined flooding has been achieved good displacement result in the pilot of Third fault block in Yangsanmu oil field, and accumulated types of important parameters and optimum plans, this technology of combined flooding is expected to increase recovery ratio by 4.77%.

Real-time data processing of interferential imaging spectrometer based on FPGA

Based on the data processing technologies of interferential spectrometer, a sort of real-time data processing system on chip of interferential imaging spectrometer was studied based on large capacitance and high speed field programmable gate array( FPGA) device. The system integrates both interferograrn sampling and spectrum rebuilding on a single chip of FPGA and makes them being accomplished in real-time with advantages such as small cubage, fast speed and high reliability. It establishes a good technical foundation in the applications of imaging spectrometer on target detection and recognition in real-time.

A Label-Free Multisensing Immunosensor Based on Imaging Ellipsometry

An immunosensor based on imaging ellipsometry and its potential applications was demonstrated in this paper. It has been proven a fast, reliable, and convenient method to quantify the thickness distribution of protein layers or detect protein concentration in solution. Combined with a protein chip, the immunosensor was able to detect multiple analytes simultaneously without any labeling. Preliminary results demonstrated how this immunosensor could be used to monitor several independent biospecific binding processes in real-time and in situ conditions.

Biosensor with Total Internal reflection Imaging Ellipsometry

In order to monitor multiple protein reaction processes simultaneously, a biosensor based on imaging ellipsometry operated in the total internal reflection mode is proposed. It could be realised as an automatic analysis for protein interaction processes with real-time label-free method. Its principle and methodology as well as a demonstration for its applications are presented.

Development Of Biosensor Based On Imaging Ellipsometry

The concept of biosensor based on imaging ellipsometry was proposed ten years ago. Its principle and the methodology as well as some solutions to problems which have to be faced during the development are mentioned. Its properties of phase sensitive, high throughput and fast sampling, as well as label-free, sensitivity better than 1 ng/ml for Immunoglobulin G, and real-time analysis for protein interaction process, etc. provide a potential for applications in biomedicine field. The recent biosensing development with total internal reflection imaging ellipsometry is presented also. [GRAPHICS] An example of 48 protein arrays in matrix. (C) 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Visualization of molecule interaction between antigen and antibody - One of ellipsometric imaging applications

It is to investigate molecule interactions between antigen and antibody with ellipsometric imaging technique and demonstrate some features and possibilities offered by applications of the technique. Molecule interaction is an important interest for molecule biologist and immunologist. They have used some established methods such as immufluorcence, radioimmunoassay and surface plasma resonance, etc, to study the molecule interaction. At the same time, experimentalists hope to use some updated technique with more direct visual results. Ellipsometric imaging is non-destructive and exhibits a high sensitivity to phase transitions with thin layers. It is capable of imaging local variations in the optical properties such as thickness due to the presence of different surface concentration of molecule or different deposited molecules. If a molecular mono-layer (such as antigen) with bio-activity were deposited on a surface to form a sensing surface and then incubated in a solution with other molecules (such as antibody), a variation of the layer thickness when the molecules on the sensing surface reacted with the others in the solution could be observed with ellipsometric imaging. Every point on the surface was measured at the same time with a high sensitivity to distinguish the variation between mono-layer and molecular complexes. Ellipsometric imaging is based on conventional ellipsometry with charge coupled device (CCD) as detector and images are caught with computer with image processing technique. It has advantages of high sensitivity to thickness variation (resolution in the order of angstrom), big field of view (in square centimeter), high sampling speed (a picture taken within one second), and high lateral resolution (in the order of micrometer). Here it has just shown one application in study of antigen-antibody interaction, and it is possible to observe molecule interaction process with an in-situ technique.

The Development of Biosensor with Imaging Ellipsometry

The concept of biosensor with imaging ellipsometry was proposed about ten years ago. It has become an automatic analysis technique for protein detection with merits of label-free, multi-protein analysis, and real-time analysis for protein interaction process, etc. Its principle, andrelated technique units, such as micro-array, micro-fluidic and bio-molecule interaction cell, sampling unit and calibration for quantitative detection as well as its applications in biomedicine field are presented here.

Imaging and tracking single fluorescent nanobeads in solution

In single-particle tracking (SPT), fluorescence video microscopy is used to record the motion images of single particle or single molecule. Here, by using a total-internal-reflection microscope equipped with an argon ion laser and a charge-coupled device (CCD) camera with high-speed and high-sensitivity, video images of single nanobeads in solutions were obtained. From the trajectories, the diffusion coefficient of individual nanobead was determined by the mean square displacements as a function of time. The sizes of nanobeads were calculated by Stokes-Einstein equation, and the results were compared with the actual values.

Subwavelength imaging with two symmetrical interfaces by dielectric-tube photonic crystals

As distinct from coated photonic crystals, in this paper we propose a novel one that is made of dielectric tubes arranged in a close-packet square lattice. Without metallic cores, this structure is low-loss and convenient to fabricate. A left-handed frequency region is found in the second band by dispersion characteristic analysis. Without inactive modes for the transverse electric mode, negative refraction and subwavelength imaging are demonstrated by the finite-difference time-domain simulations with two symmetrical interfaces, i.e. Gamma X and Gamma M.

Range-Gated Laser Stroboscopic Imaging for Night Remote Surveillance

For night remote surveillance, we present a method, the range-gated laser stroboscopic imaging(RGLSI), which uses a new kind of time delay integration mode to integrate target signals so that night remote surveillance can be realized by a low-energy illuminated laser. The time delay integration in this method has no influence on the video frame rate. Compared with the traditional range-gated laser imaging, RGLSI can reduce scintillation and target speckle effects and significantly improve the image signal-to-noise ratio analyzed. Even under low light level and low visibility conditions, the RGLSI system can effectively work. In a preliminary experiment, we have detected and recognized a railway bridge one kilometer away under a visibility of six kilometers, when the effective illuminated energy is 29.5 mu J.