12 resultados para Swap spreads
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
The SWAP operation in a two-qubit Heisenberg model in the presence of Dzyaloshinskii-Moriya (DM) anisotropic antisymmetric interaction is investigated. 1t is shown that the SWAP operation can be implemented for some kinds of DM coupling and the influence of DM couplings is divided into different cases. The conditions of the DM coupling under which the SWAP operation is feasible are established. (C) 2007 Elsevier B.V. All rights reserved.
Resumo:
In this paper we study the SWAP operation in a two-qubit anisotropic XXZ model in the presence of an inhomogeneous magnetic field. We establish the range of anisotropic parameter lambda within which the SWAP operation is feasible. The SWAP errors caused by the inhomogeneous field are evaluated.
Resumo:
The exhaust gases from industrial furnaces contain a huge amount of heat and chemical enthalpy. However, it is hard to recover this energy since exhaust gases invariably contain combustible components such as carbon monoxide (CC). If the CO is unexpectedly ignited during the heat recovery process, deflagration or even detonation could occur, with serious consequences such as complete destruction of the equipment. In order to safely utilize the heat energy contained in exhaust gas, danger of its explosion must be fully avoided. The mechanism of gas deflagration and its prevention must therefore be studied. In this paper, we describe a numerical and experimental investigation of the deflagration process in a semi-opened tube. The results show that, upon ignition, a low-pressure wave initially spreads within the tube and then deflagration begins. For the purpose of preventing deflagration, an appropriate amount of nitrogen was injected into the tube at a fixed position. Both simulation and experimental results have shown that the injection of inert gas can successfully interrupt the deflagration process. The peak value of the deflagration pressure can thereby be reduced by around 50%. (C) 2008 Elsevier Ltd. All rights reserved.
Resumo:
5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescence in-situ hybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp.
Resumo:
Two-dimensionally arranged gold rings were prepared by depositing a polymeric membrane bearing a dense array of uniform pores onto a mica substrate, filling the pores with a solution of a gold precursor, evaporation of the solvent and calcinations. The epitaxy of gold rings is confirmed by x-ray diffraction measurements, and the epitaxial relationship between gold rings and the mica was found to be Au(111)[1-10]parallel to mica(001)[010]. The polar and azimuthal angular spreads are 0.3 degrees and 1 degrees, respectively, which is at least equal to or better than the quality of the corresponding epitaxial gold-film on mica. (c) 2005 American Institute of Physics.
Resumo:
天然免疫分子TRIM5α(tripartite motif protein 5α)是近年来发现的一种重要的宿主细胞内逆转录病毒限制因子。在灵长类动物细胞中,TRIM5α蛋白可以在病毒进入细胞后、逆转录前的阶段抑制HIV-1、N-MLV和EIAV等逆转录病毒的复制。由于TRIM5α分子的作用,绝大部分旧大陆猴(Old World monkey)都不能感染HIV-1。而在新大陆猴(New World monkey)中,鹰猴是唯一不感染HIV-1的灵长类动物。研究证明,鹰猴细胞中存在的TRIM5-CypA融合蛋白(owl monkey TRIM5-CypA,omTRIMCyp)介导了抗HIV-1的作用,从而使鹰猴不能感染HIV-1。研究证明,平顶猴是旧大陆猴中唯一报道可以感染HIV-1的灵长类动物,但是其感染HIV-1的机制并不清楚。根据现行的灵长类动物分类学,原属平顶猴群体(M. nemestrina group)的三个亚种分为猕猴属的三个不同种:巽他平顶猴(Sunda pig-tailed macaque,M. nemestrina),北平顶猴(Northern pig-tailed macaque,M. leonina)和明打威猴(Mentawai macaque,M. pagensis)。本论文对中国云南境内北平顶猴TRIM5基因座和感染HIV-1的相关性进行了研究。通过PCR和测序对北平顶猴基因组TRIM5基因座进行分析,发现一个CypA假基因的cDNA通过逆转座机制插入至TRIM5基因座的3’-UTR区域,形成了一个不同于鹰猴TRIM5-CypA的新型融合基因npmTRIMCyp(northern pig-tailed macaque TRIM5-CypA)。通过RT-PCR对npmTRIMCyp融合基因的转录本进行分析,我们鉴定出npmTRIMCyp共有3种不同的选择性剪接产物,分别为npmTRIMCypV1-V3。进一步克隆和测序这3种不同选择性剪接体,通过丰度和序列分析证实:npmTRIMCypV2是优势剪接体,可能在该融合基因产物的功能中发挥作用。研究发现北平顶猴npmTRIMCyp融合基因主要转录本中外显子7和8均被剪切掉。外显子7剪接丢失机制源于TRIM5第6内含子内 3’剪接位点的G/T突变。我们克隆了npmTRIMCyp融合基因cDNA的蛋白编码区ORF,并构建了重组表达npmTRIMCyp的载体,转染HeLa和HeLa-T4细胞并获得稳定表达的细胞株。通过感染HIV-1证实,npmTRIMCyp融合蛋白不能够限制HIV-1的感染和复制,这可能是北平顶猴作为旧大陆猴中唯一对HIV-1易感的灵长类动物的重要分子机制之一。通过HIV-1感染灵长类动物PBMCs实验证实,北平顶猴可以感染HIV-1。npmTRIMCyp可以有效地限制HIV-2ROD的复制,但对SIVmac239只有十分微弱的限制活性。通过构建鹰猴omTRIMCyp和北平顶猴npmTRIMCyp的置换剪接体(SWAP-1和SWAP-2),转染融合基因及其置换剪接体的CRFK细胞激光共聚焦实验证明,npmTRIMCyp、SWAP1和SWAP2在细胞内主要存在于胞浆中。稳定表达融合蛋白和置换剪接体的CRFK细胞感染HIV-1-GFP-VSVG分析表明,含omTRIMCyp外显子7的SWAP-1和SWAP-2均具有限制HIV-1活性,但SWAP-1的活性更强一些,这表明TRIM5结构域的外显子7可能在介导对HIV-1的限制活性中发挥了协同辅助作用。免疫共沉淀研究表明,npmTRIMCyp不能识别和结合HIV-1的衣壳蛋白。对北平顶猴中介导识别逆转录病毒区域的基因组部分进行了测序,共鉴定出46个多态性位点,表明在北平顶猴识别逆转录病毒衣壳区域存在较高的多态性。
Resumo:
An augmented immersed interface method (IIM) is proposed for simulating one-phase moving contact line problems in which a liquid drop spreads or recoils on a solid substrate. While the present two-dimensional mathematical model is a free boundary problem, in our new numerical method, the fluid domain enclosed by the free boundary is embedded into a rectangular one so that the problem can be solved by a regular Cartesian grid method. We introduce an augmented variable along the free boundary so that the stress balancing boundary condition is satisfied. A hybrid time discretization is used in the projection method for better stability. The resultant Helmholtz/Poisson equations with interfaces then are solved by the IIM in an efficient way. Several numerical tests including an accuracy check, and the spreading and recoiling processes of a liquid drop are presented in detail. (C) 2010 Elsevier Ltd. All rights reserved.
Resumo:
This study provides a useful biodosimetry protocol for radiation accidents that involve high doses of heavy particle radiation. Human peripheral blood lymphocytes (PBLs) were irradiated in vitro with high doses (5–50 Gy) of charged heavy-ion particles (carbon ions, at an effective linear-energy-transfer (LET) of 34.6 keV/ m), and were then stimulated to obtain dividing cells. PBLs were treated with 100nMcalyculin A to force chromosomes to condense prematurely, and chromosome spreads were obtained and stained with Giemsa. The G2 prematurely condensed chromosome (G2-PCC) index and the number of G2-PCC including fragments (G2-PCC-Fs) per cell for each radiation dose point were scored. Dose-effect relationships were obtained by plotting the G2-PCC indices or G2-PCC-Fs numbers against radiation doses. The G2-PCC index was greater than 5% up to doses of 15 Gy; even after a 30Gy radiation dose, the index was 1 to 2%. At doses higher than 30 Gy, however, the G2-PCC indices were close to zero. The number of G2-PCC-Fs increased steeply for radiation doses up to 30 Gy at a rate of 1.07 Gy−1. At doses higher than 30 Gy, the numbers of G2-PCC-Fs could not be accurately indexed because of the limited numbers of cells for analysis. Therefore, the number of G2-PCC-Fs could be used to estimate radiation doses up to 30 Gy. In addition, a G2-PCC index close to zero could be used as an indicator for radiation doses greater than 40 Gy.
Resumo:
利用SWAP(Soil-Water-Atmosphere-Plant)模型对黄土高原水蚀风蚀交错区坡地土壤-植被-大气系统中的水循环进行数值模拟。结果显示,SWAP模型很好的模拟了不同土地利用方式条件下的土壤水循环过程。根据模拟结果,水蚀风蚀交错区的丰水年份,农地和种植第一年的紫花苜蓿地季末土壤水分稍有盈余,谷子和紫花苜蓿的日蒸散量分别为1.2~2.6 mm和1.2~2.5 mm。
Resumo:
To develop genetic and physical maps for shrimp, accurate information on the actual number of chromosomes and a large number of genetic markers is needed. Previous reports have shown two different chromosome numbers for the Pacific whiteleg shrimp, Penaeus vannamei, the most important penaeid shrimp species cultured in the Western hemisphere. Preliminary results obtained by direct sequencing of clones from a Sau3A-digested genomic library of P. vannamei ovary identified a large number of (TAACC/GGTTA)-containing SSRs. The objectives of this study were to (1) examine the frequency of (TAACC)(n) repeats in 662 P. vannamei genomic clones that were directly sequenced, and perform homology searches of these clones, (2) confirm the number of chromosomes in testis of P. vannamei, and (3) localize the TAACC repeats in P. vannamei chromosome spreads using fluorescence in situ hybridization (FISH). Results for objective I showed that 395 out of the 662 clones sequenced contained single or multiple SSRs with three or more repeat motifs, 199 of which contained variable tandem repeats of the pentanucleotide (TAACC/GGTTA),, with 3 to 14 copies per sequence. The frequency of (TAACC)n repeats in P. vannamei is 4.68 kb for SSRs with five or more repeat motifs. Sequence comparisons using the BLASTN nonredundant and expressed sequence tag (EST) databases indicated that most of the TAACC-containing clones were similar to either the core pentanucleotide repeat in PVPENTREP locus (GenBank accession no. X82619) or portions of 28S rRNA. Transposable elements (transposase for Tn1000 and reverse transcriptase family members), hypothetical or unnamed protein products, and genes of known function such as 18S and 28S rRNAs, heat shock protein 70, and thrombospondin were identified in non-TAACC-containing clones. For objective 2, the meiotic chromosome number of P. vannamei was confirmed as N = 44. For objective 3, four FISH probes (P1 to P4) containing different numbers of TAACC repeats produced positive signals on telomeres of P. vannamei chromosomes. A few chromosomes had positive signals interstitially. Probe signal strength and chromosome coverage differed in the general order of P1 > P2 > P3 > P4, which correlated with the length of TAACC repeats within the probes: 83, 66, 35, and 30 bp, respectively, suggesting that the TAACC repeats, and not the flanking sequences, produced the TAACC signals at chromosome ends and TAACC is likely the telomere sequence for P. vannamei.
Resumo:
With high-resolution conductivity-temperature-depth (CTD) observations conducted in Oct.-Nov. 2005, this study provides a detailed quasi-synoptic description of the North Pacific Tropic Water (NPTW), North Pacific Intermediate Water (NPIW) and Antarctic Intermediate Water (AAIW) in the western North Pacific. Some novel features are found. NPTW enters the western ocean with highest-salinity core off shore at 15 degrees-18 degrees N, and then splits to flow northward and southward along the western boundary. Its salinity decreases and density increases outside the core region. NPIW spreads westward north of 15 degrees N with lowest salinity off shore at 21 degrees N, but mainly hugs the Mindanao coast south of 12 degrees N. It shoals and thins toward the south, with salinity increasing and density decreasing. AAIW extends to higher latitude off shore than that in shore, and it is traced as a salinity minimum to only 10 degrees N at 130 degrees E. Most of the South Pacific waters turn northeastward rather than directly flow northward upon reaching to the Mindanao coast, indicating the eastward shift of the Mindanao Undercurrent (MUC).
Resumo:
South China Sea is located in the convergence of Eurasian plate, the Pacific Ocean plate and Indian Ocean-Australia plate. The total area is about 3,500,000 km2, the geologic structure is complicated, and the structure line cut off reciprocal is the marginal sea taking form by that the seafloor spreads during the middle Oligocene. South China Sea continental margin have developed more than 10 large oil-gas bearing basins and a number of medium-small sized basins. These basins contain abundant mineral resources such as oil & gas. The marginal deepwater area in the north part of South China Sea has become our country’s strategic energy prospecting frontier. The deepwater area of Zhujiangkou and Qiongdongnan basins is the research target in this thesis. The thesis studied deep structure and the earth dynamics of the north part of South China Sea margin, and these researches provide scientific basis for oil-gas resources strategic investigation and valuation in deepwater sea area of north part slope of South China Sea. In order to develop the research of rebuilding velocities and density architecture of earth shell in region of interest, in marginal deepwater area in the north part of South China, we adopted 14 long-cable seismic reflection profile data of 3556.41 kilometers in total, the gravity measurement data along profiles (3851.44 kilometers in total), the magnetic observation along profiles (3838.4 kilometers in total) and depth measurement along profile, the logging data of 11 wells in project, the interpreted fault parameter and preexisting geologic and geophysical research achievement. This thesis has carried out concretely studying research as follows: 1. Overlay-velocity data sampling and analysis, interval velocity calculation, time-depth conversion, model building of earth shell velocity and layering character of earth shell are studied on 14 deep sections. Velocity structure in region of interest has revealed: Changchang is the sag with thinnest crust in Qiongdongnan basin; the sedimentary thickness lowers gradually from north to south, and the thickness change from west to east is milder. The sags’ sedimentary velocities in Qiongdongnan basin have obvious demarcation. The velocity of the 8000 meters sedimentary rocks is 4700 m/s in Shunde sag and Baiyun sag, and is the lowest; at that depth, the velocity very different in Liwan sag and Baiyun sag, which is about 800m/s. 2. Extracting gravity data and building of initial crust density model along the section; With Bouguer gravity anomaly data as constraint, revising density distributes of initial model, and building the crust density model. 3. With crust velocity and density as constraint, correcting the effect of thermobaric field and constructing constitution structure of rock in region of interest. By this research, we known that rocks in Zhujiangkou upper crustal layer are chiefly granite-gneiss, quartzite, granodiorite and basalt, however, rocks in Qiongdongnan basin upper earth shell are chiefly composed of granite-gneiss, quartzite, granodiorite, diorite and basalt. 4. Synthetically crust velocity and density structure, gaining expanding factor on crust and entire crust along section. The result is indicated: the expanding factor in every sag rises from northwest to southeast, which have reflected thinning characteristic of crust from continent to ocean. Intra-crustal deformation degree in Changchang and Ledong-Lingshui sag is bigger than that in Songnan-Baodao sag. Entire crust extension factor in Changchang and Songnan-Baodao sag is greater than that in Ledong-Lingshui sag, which can make an explanation of frequently event and longer heating process in middle-east of Qiongdongnan basin. 5. Synthesize multidisciplinary information to discuss the earth dynamics significance of discordogenic seismic profile in deepwater area of Zhujiangkou and Qiongdongnan basins.