黄土丘陵区纸坊沟流域农业生态经济安全评价

以近70年纸坊沟流域农业生态经济系统演变过程为基础,利用国家"九五"科技攻关项目中所提出的评价指标体系,对纸坊沟流域不同发展阶段标志年份的农业生态经济安全状况进行了评价,得出:农业生态经济系统在1938、2000、2005年处于亚安全状态,1958、1975年处于极端不安全状态,1985、19901、995年处于不安全状态。通过对农业生态经济安全变化驱动力的分析,预测了纸坊沟流域农业生态经济安全的发展趋势,提出了黄土丘陵区实现农业生态经济安全的策略:以培育主导产业为中心,完善农业系统循环链网,调整产业结构,实现农业资源的有效、永续利用。

Cytotoxicity evaluation of three pairs of hexabromocyclododecane (HBCD) enantiomers on Hep G2 cell

Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure alpha-, beta-, and gamma-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-P-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was gamma-HBCD >= beta-HBCD > alpha-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) alpha-, (+) beta- and (+) gamma-HBCD) than the corresponding (-)-forms ((-) alpha-, (-) beta- and (-) gamma-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers. (C) 2008 Published by Elsevier Ltd.