A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling

A novel short neurotoxin, cobrotoxin c (CBT C) was isolated from the venom of monocellate cobra (Naja kaouthia) using a combination of ion-exchange chromatography and FPLC. Its primary structure was determined by Edman degradation. CBT C is composed of 61 amino acid residues. It differs from cobrotoxin b (CBT B) by only two amino acid substitutions, Thr/Ala11 and Arg/Thr56, which are not located on the functionally important regions by sequence similarity. However, the LD50 is 0.08 mg/g to mice, i.e. approximately five-fold higher than for CBT B. Strikingly, a structure-function relationship analysis suggests the existence of a functionally important domain on the outside of Loop III of CBT C. The functionally important basic residues on the outside of Loop III might have a pairwise interaction with alpha subunit, instead of gamma or delta subunits of the nicotinic acetylcholine receptor (nAChR). (C) 2002 Elsevier Science Inc. All rights reserved.

从酵母菌中获得眼镜蛇毒神经多肽菌株及其获得方法

发明为一种从酵母菌中获得眼镜蛇毒神经多肽菌株及其获得方法，主要是通过从天然眼镜蛇毒神经多肽所测定的一级结构及酵母偏爱的密码子设计的全基因序列，利用基因载体pPIczα，构建的重组质粒pPIczα－Cbt，并获得在该酵母菌中的高效表达产物。该菌株和天然眼镜蛇毒神经多肽有同样的结构和效果，可以替代天然眼镜蛇毒神经多肽，可以满足逐渐增加的市场需求，又能保护蛇资源，是一种有应用前景的菌株。

从大肠杆菌中获得眼镜蛇毒神经多肽菌株及其获得方法

本发明为一种从大肠杆菌中获得眼镜蛇毒神经多肽菌株及其获得方法，主要是通过从天然眼镜蛇毒神经多肽所测定的一级结构，利用基因载体pET21b，构建的重组质粒pET21b-Cbt，转化至大肠杆菌BL21中，而获得能高效表达眼镜蛇毒神经多肽的菌株。该方法具有直接表达、分离纯化工艺简便、活性高等特点。该菌株的表达产物和天然眼镜蛇毒神经多肽有同样的结构和活性，可以替代天然眼镜蛇毒神经多肽，可以满足逐渐增加的市场需求，又能保护蛇资源，是一种有应用前景的表达菌株。

Effects of tetrabrominated diphenyl ether and hexabromocyclododecanes in single and complex exposure to hepatoma HepG2 cells

This study was designed to determine cytotoxic effects of PBDE-47 and HBCDs individually or with a mixture of both compounds exposure to Hep G2 cells. The results showed PBDE-47 and HBCDs induced increase of nitric oxide synthase (NOS) activity, release of NO. dissipation of mitochondria membrane potential and cell apoptosis. Exposure to HBCDs induced ROS formation. Moreover, preincubation with PTIO (NO scavanger) and N-acetylcysteine (ROS scavanger) partially reversed cytotoxic effects of these compounds. The possible mechanism is that PBDE-47 and HBCDs could boost generation of NO and/or ROS, impact mitochondria, and result in start-ups of apoptosis program. Cells exposed to mixture of both compounds and each of them showed non-apoptotic rate significant difference, but the combination of them caused more adverse effects on cells. These results Suggest that PBDE-47 and HBCDs in single and complex exposure have the cytotoxic activity of anti-proliferation and induction of apoptosis in tumor cells in vitro. (C) 2008 Elsevier B.V. All rights reserved.

面向信息系统工程监理的软件工具IT-PM

在信息系统工程开发中引入监理机制已经成为业界的共识。监理组织以独立“第三方”身份加入项目,通过“控制、管理、协调”的手段,达到提高项目质量的目的。分析了信息系统工程监理自动化支持的研究现状,提出了面向信息系统工程监理的软件工具IT-PM。工具以信息系统工程监理的过程模型为基础,支持监理工作的流程和信息管理自动化。

基于PC的一种新型现场总线控制系统

PC性能的不断提高及实时操作系统内核的出现,促使了一种基于PC和现场总线的新型控制系统的诞生。由于该系统成本低,占用空间少,在联网、监控等方面有突出优点,并且PC机性能已经非常可靠,所以它可以取代传统的PLC来完成实时控制任务。为了验证系统的可行性,文章系统地介绍了这类新型控制系统的优点和整体软硬件结构,并对其中一个重要的性能指标——响应时间,进行了详细分析,然后通过一个实际系统——自动传输线系统,验证了这一控制系统的可行性。

虚拟现实辅助机器人遥操作技术研究

本文以水下机器人的遥操作作业为应用背景 ,提出并实现了虚拟现实技术和视觉感知信息辅助机器人遥操作实验系统 .该系统使用了 CAD模型和立体视觉信息完成遥操作机器人及其作业环境的几何建模和运动学建模 ,实现了虚拟作业环境的生成和实时动态图形显示 .采用了基于立体视觉的虚拟环境与真实环境的一致性校正、图形图像叠加、作业体与环境位姿关系建立、基于网络的监控通讯等关键技术 .在这个实验系统中 ,操作人员可利用所生成的虚拟环境 ,在多视点、多窗口作业状态图形和图像显示帮助下 ,实时动态地进行作业观测与机器人遥操作与运动规划 ,为先进遥操作机器人系统的实现提供了经验和关键技术 .

一种欠驱动AUV的三维实时避碰方法

针对以测距声纳为避碰传感器的一类欠驱动型AUV，提出了一种水平面和垂直面相结合的三维实时避碰方法。根据测距声纳和欠驱动AUV 的特殊性，首先从运动规划和路径规划2 个层次提出了AUV 混合型实时避碰结构，并分别设计了基于事件反馈监控的避碰自动机和基于免疫遗传的局部路径规划算法。多种典型障碍场景的半物理仿真实验表明，论文所提方法能够实现AUV 安全、稳定的三维避碰过程。

汽车变速器装配生产线监控系统的设计

生产监控系统在企业生产管理中具有重要的不可替代的地位。网络化生产监控系统适应了企业管控一体化发展的要求,它可以直接与企业上层管理信息系统相融合,使得管理人员可以直观的了解到生产现场细致的生产数据,从而可以在宏观和微观两个方面来把握企业生产、计划、调度、管理。本文以现场总线技术和OPC技术为理论基础,提出了一种网络化监控系统架构,并讲述了其功能,着重阐述了系统功能的设计和实现。

港西油田现代油藏管理研究与实践

Through years of practice, reservoir management has already become the basic mode of foreign oil companies to realize the high-efficient development of the oil field. From the view of reservoir development and technological economy, reservoir management regards the study of the reservoir engineering, designs of reservoir projects and the dynamic analysis of the reservoir's performance as a system. In the fields of reservoir description, the establishment of the geological models and development models, the dynamic simulations of reservoir exploitation and the design of the oil engineering, reservoir management emphasizes the cooperation of the geology and the engineering, the combination of the engineering technology and the economic evaluation. In order to provide the means and basis for the reservoir geology study, reservoir evaluation, reserves calculation, numerical simulation, development plan and risk analysis, it adopts the reservoir management activities(team work) to make and implement the optimized oil field development management strategies so that secientific and democratic decision making can be achieved. Under the planned economic system for a long time, the purpose of Chinese reservoir development has been to fulfill the" mandatory" production task. With the deepening of the reform, the management organization of Chinese petroleum enterprises has been gradually going through the transition and reforms to the operational entity and the establishment of the mode of oil companies under the socialist market economy system. This research aims at introducing the advanced reservoir management technique from foreign countries to further improve the reservoir development results and wholly raise the economic benefits of Chinese mature land facieses sandstone reservoirs in the later stage of the water flooding. We are going to set up a set of modern reservoir management modes according to the reservoir features, current situation and existing problems of GangXi oil field of DaGang oil company. Through the study and implementation of the reservoir description and numerical simulation technology effectively, we plan to work out integrated adjustment projects, to study the related technology of oil recovery; to set up the effective confirmable data procedure and data management system of the reservoir management, to establish the coordinated model and workbench related to geology, engineering and economy in order to realize the real time supervision and evaluation on the process of reservoir development. We hope to stipulate modernization management tools for GangXi oil fields to rationally utilize various kinds of existing technological methods and to realize the economic exploitation and achieve the maximum benefits from the reservoir. The project of the modem reservoir management will be carried out on the GangXi oil field of DaGang oil company for this oil field is typical and has integrated foundamental materials and perfect networks. Besides, it is located in the good geographical position enjoying very convenient traffic. Implementing modern reservoir management will raise the recovery ratio, reduce the production cost and improve the working efficiency. Moreover, the popularization of modern reservoir management will improve the comprehensive benefits of DaGang oil company and even the whole Petro China. Through the reserch of this project, the following technical indicators can be reached: Establishing the concept of modern reservoir management. Establishing a set of integrated data information management system adapt to the features of GangXi reservoir. 3. Forming technical research modes of modern reservoir management suitable for mature reservoirs in the later developing stage. 4. Advancing projects of GangXi reservoir which are maxium optimized in engineering technique and economic benefits of oil exploitation. Besides, this set of technology, research principle and method can guide the mature reservoir of DaGang oil field and even the whole PetroChina to develop the further research of reservoir adjustment and improve the reservoir recovery factor and developing level constantly.

中老年人工作记忆与血管性疾病及危险因素的相关分析

Background With the increasing of an elderly population, the number of both vascular disease and cognitive function is increasing. Recent study shown that there is close relationship between vascular disease or vascular risk factors and cognitive function, but many questions are unclear. Objective The aim of this study was to explore the status of cognitive function of midlife and elderly subjects and to evaluate association between vascular risk factors and cognitive function. Methods We selected 330(female,128,male,202) retire navy veterans aged between 55 and 87 years old in Beijing. The average age was 67 years old. Data about vascular risk factors, including: history of cerebral infarction, cardiovascular disease, hypertension, diabetes mellitus, carotid lesions, cigarette smoking, drinking, obesity, and so on. Cognitive functions were assessed by neuropsychological tests: the four tasks of working memory. Results The univariate analysis indicated that there were significant differences of working memory scores in aging, gender, education level, the history of stroke, hypertension with midlife and elderly subjects. Moderate levels of alcohol intake maybe better for cognition. Conclusion vascular disease and some vascular risk factors may lead to cognitive function decline, thus, intervention methods should be carried out in middle and elderly people with vascular risk fact should be under special supervision, with at least annual neuropsychological evaluation. Key words: cognitive function; vascular risk factor， working memory.

空间工作记忆与注意广度

The relationship between working memory (WM) and attention has attracted many researchers. In his embedded-processes model of WM, Cowan (1999, 2001) uses the term focus of attention to refer to the core component of WM, and proposes that the focus of attention of WM and that of perception have the same span, which is a fixed number. This hypothesis about the scope of attention has seldom been tested, although considerable studies have revealed that WM and attention have overlapping mechanisms. The present dissertation tests this hypothesis by examining the dual-task interference between Corsi Blocks Task (CBT) and Mutilple Object Tracking (MOT) and the findings demenstrate that Cowan’s hypothesis is not exactly true. The results of our first study show that the interference effect of MOT on CBT is a reliable indicator of whether and to which extent the attentional resoureces of WM and perception overlap. In the second study we find that the capacity of the common resources is not a fixed number but varies with the difficulty of control of attention. And the third study indicates that attentional resources used in WM and perception are partly independent, the overlapping part can attend to only one or two items or locations at a time. These findings can contribute to future studies on the capacity limitation of different cognitive functions, and to the development of relevant ability tests.