In vitro anti-HIV and -HSV activity and safety of sodium rutin sulfate as a microbicide candidate

Sodium rutin sulfate (SRS) is a sulfated rutin modified from the natural flavonol glycoside rutin. Here, we investigated its in vitro anti-HIV and -HSV activities and its cytotoxic profile. Fifty percent inhibitory concentration (IC50) values of SRS against HIV-1 X4 virus IIIB, HIV-1 R5 isolates Ada-M and Ba-L were 2.3 +/- 0.2, 4.5 +/- 2.0 and 8.5 +/- 3.8 mu M with a selectivity index (SI) of 563, 575 and 329, respectively. Its IC50 against primary R5 HIV-1 isolate from Yunnan province in China was 13.1 +/- 5.5 mu M, with a Sl of 197. In contrast, unsulfated rutin had no activity against any of the HIV-1 isolates tested. Further study indicated that SRS blocked viral entry and virus-cell fusion likely through interacting with the HIV- I envelope glycoprotein. SRS also demonstrated some activity against human herpes simplex virus (HSV) with an IC50 of 88.3 +/- 0.1 mu M and a Sl of 30. The 50% cytotoxicity concentration (CC50) of SRS was >3.0 mM, as determined in human genital ME 180, HeLa and primary human foreskin fibroblast cells. Minimum inhibitory concentration of SRS for vaginal lactobacilli was >3.0 mM. These results collectively indicate that SRS represents a novel candidate for anti-HIV-1/HSV microbicide development. (C) 2007 Elsevier B.V. All rights reserved.

Safety of tetanus toxoid in pregnant women: A hospital-based case-control study of congenital anomalies

Reported are the results of the Latin American Collaborative Study of Congenital Malformations (ECLAMC), a hospital-based case-control study of 34 293 malformed and 34 477 matched nonmalformed newborn controls. No statistical differences were found between the malformed and control groups, exposed or not exposed to tetanus toroid.

注意对驾驶场景客体-位置联系内隐学习的影响

The purpose of this study is to investigate the influence of attention resourse requirement and allocation on implicit memory and explicit memory for object-location associations in driving situation based on Adams theory on the function of implicit knowledge in the Situation Awareness(SA). This study adopted Musen’s implicit learning of object-location associations, sysmemtly manipulated the type and difficuty of the naming task. This research includes three studies and ten experiments. Their aim are separately to explore the influence of attention on implicit and explicit memory for object-loction assocaitons in simple stimulus and the driving situation. And it is needed to confirme the condition and the influencing factors of implicit memory for car-location association in different condition. It is also our aim to explore the feasibility of introduce of implicit learning methods in SA measurement. The results indicted that: ⑴ The influence of attention resourse allocation ,the difficulty of naming task , the deepness of processing on on implicit memory for object-location associations in driving situation are different . the dissociated results support the standpoint that there are two independent knowledge system; ⑵ The type of naming task more influenced the implicit and explicit memory for object-location associations than the difficulty of the naming task. The attention resourse requirement of the different type can not be compared; ⑶ The implicit memory seldom appears in the location naming task resulted from the defiency of processing on object-location association, and not as a results of the overtaxed; ⑷ The reaction time methods in the implicit learning could be used in SA measurement , it is a complementarity of the existing explicit SA measurement. These findings not only contribute to resolve ongoing debates about the process of cognition and mechanism of SA structure, but also have significant practical application in traffic safety.