2 resultados para Rita Mestokosho

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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本文以菩提树为培养对象,采用改进的液驱式间歇浸没培养技术进行组织培养实验。为了更准确,精密地控制培养条件我们研制了间歇浸没式植物培养箱;为了提高培养效率,降低成本,我们对现有培养系统和方法进行了改进。鉴于液驱式间歇浸没培养半开放等培养特点,容易造成雪崩式污染。通过调查污染来源,研究各种物理化学生物杀菌抑菌方法的特点,以及对各个培养环节的消毒能力,建立了一套适合菩提树间歇浸没无糖培养的污染控制操作规程,为进一步大规模的商业化生产打下坚实的基础。 研究结果表明, 1、利用液驱式间歇浸没系统装置(简写为TD-TIB)进行组织培养,效率更高,成本更低。 液驱式间歇浸没培养与RITA和TIB相比,简单易操作,能耗低,效率高,成本低。与间歇浸没式植物培养箱配合使用,能够精密控制环境培养参数,包括温湿度,二氧化碳氧气的全程自动化检测控制 ,能够实现远程实时控制。利用液驱式培养方法生根后的培养苗自养光合能力强,驯化时间短,成活率高,芽多,健壮。 2、操作规程能够控制半开放间歇浸没培养中的污染问题。 从对紫外,臭氧,酒精,抗生素,杀菌剂以及过滤等污染控制方法的研究我们得出,任何一种消毒杀菌方法都不能控制系统的污染,只有结合培养系统环节和消毒方法的特点,把各种方法有机组合起来才能控制半开放条件下的污染。预处理是整个控制过程的关键。按照该操作规程,在2min/2h的间歇频率下的无糖间歇培养中,30天内成功实现了菩提树的生根驯化实验,培养液无污染。 间歇浸没培养技术与污染控制技术相结合实现了小范围的规模化培养,为大规模自动化组织培养技术奠定了坚实的基础。

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Under strong ocean surface wind conditions, the normalized radar cross section of synthetic aperture radar (SAR) is dampened at certain incident angles, compared with the signals under moderate winds. This causes a wind speed ambiguity problem in wind speed retrievals from SAR, because two solutions may exist for each backscattered signal. This study shows that the problem is ubiquitous in the images acquired by operational space-borne SAR sensors. Moreover, the problem is more severe for the near range and range travelling winds. To remove this ambiguity, a method was developed based on characteristics of the hurricane wind structure. A SAR image of Hurricane Rita (2005) was analysed to demonstrate the wind speed ambiguity problem and the method to improve the wind speed retrievals. Our conclusions suggest that a speed ambiguity removal algorithm must be used for wind retrievals from SAR in intense storms and hurricanes.