Polymerase chain reaction based C4AQ0 and C4BQ0 genotyping: association with systemic lupus erythematosus in southwest Han Chinese

Objective: To investigate the association of complement C4 null genes (C4QO, including C4AQO and C4BQO) and C2 gene with systemic lupus erythematosus (SLE) in southwest Han Chinese; 136 patients with SLE and 174 matched controls were genotyped. Methods: C4 null genes were determined by a polymerase chain reaction (PCR) procedure with sequence specific primers (PCR-SSP). The 2 bp insertion in exon 29, which was previously identified in non-Chinese populations and caused defective C4A genes, was directly typed by sequencing the whole exon 29 using exon specific primers. The exon 6 of complement C2 was also sequenced in both the patients and controls. Results: The frequency of homozygous C4AQO allele was 12.5% (17/136) in patients with SLE compared with 1.1% (2/174) in controls (p<0.001, odds ratio (OR)=12.286, 95% confidence interval (95% CI) 2.786 to 54.170). There was no significant difference for homozygous C4BQO allele between patients with SLE and controls (p=0.699). Patients with the C4AQO gene had an increased risk of acquiring renal disorder, serositis, and anti-dsDNA antibodies compared with those without C4AQO (for renal disorder, p=0.018, OR=8.951, 95% Cl 1.132 to 70.804; for serositis, p=0.011, OR 4.891, 95% CI 1.574 to 15.198; for anti-dsDNA, p=0.004, OR 7.630, 95%Cl 1.636 to 35.584). None of the patients or controls had the 2 bp insertion in exon 29 of the C4 gene. The type I C2 deficiency was not detected in the 3 10 samples. Conclusion: It is suggested that deficiency of C4A (not due to a 2 bp insertion in exon 29), but not C4B or C2, may be a risk factor for acquiring SLE in south west Han Chinese; this results in increased risk of renal disorder, serositis, and anti-dsDNA antibodies in patients with SLE. Racial differences seem to be relevant in susceptibility to SLE.

农民工群体偏爱的研究

The present paper studies the explicit, implicit and the neural mechanism of group bias on rural residents, off-farm workers and urban citizens in Off-farm workers. The relations between group-bias and these factors such as collective self-esteem, self-esteem, group identity and depression were also discussed. The main results of this paper can be summarized as follows： 1. There were dissociations between explicit and implicit group-bias of the Off-farm workers on all three groups. Off-farm workers favored urban citizens implicitly most, and the bias on rural residents was least;. However, the rural residents was the group they liked most, but the urban citizens was the least. 2. The more the implicit and the less the explicit in-group bias they had, the more they felt depressive. 3. Collecitve self-esteem was only related to the explicit in-group bias. 4. The undergraduates showed bias on urban citizens and prejudice to rural residents as Off-farm workers, but their attitude towards Off-farm worker was more positive than Off-farm worker themselves was. 5.The implicit group-bias occurs rapidly and automatically which includes two potential processes as activation and inhibition and it isn’t necessary for them coexist in the same object. Besides, N400 and LPC are sensitive to the two processes respectively.