Isolation and purification of phycoerythrin from red alga Garcilaria verrucosa by expanded-bed-adsorption and ion-exchange chromatogaphy

R-phycoerythrin was isolated and purified from Gracilaria verrucosa on an expanded-bed adsorption column combined with ion-exchange chromatography, which can effectively solve the problem of blockage of chromatographic columns due to polysaccharides during isolation and purification of phycobiliproteins. 0.1 M (NH4)(2)SO4 proved best to elute R-phycoerythrin from the expanded-bed column, and desalted 0.1 M (NH4)(2)SO4 eluate was used on an ion-exchange column to purify the R-phycoerythrin. Using this two-stage chromatography, the purity (OD565/OD280) of the R-phycoerythrin from G. verrucosa is increased to 4.4, and the yield of purified R-phycoerythrin can reach 0.141 mg . g(-1) of the frozen alga.

湖南省小型甲壳动物新种和新记录

本文记述了湖南省首次记录的63种小型甲壳动物。其中包括一新亚种密纹尖额溞Alona verrucosa lineolata和6个中国新记录种和亚种.将国内学者以前记载的钩足平直溞Pleuroxus hamulatus移入锐额溞属,即钩足锐额溞Alonella hamulatus。

粗疣类钱袋苔[Apomarsupellaverrucosa(Nichols.)Vana]及其新异名

对产自中国云南缺萼苔科(Gymnomitriaceae)粗疣类钱袋苔(Apomarsupella verrucosa(Nichols.)Vana)和产自尼泊尔(Gymnomitrion papillosumKitag.&S.Hatt.)的模式标本进行了对比研究,发现Gymnomitrion papillosum和粗疣类钱袋苔特征相一致,将其处理为粗疣类钱袋苔的异名。