Dynamics of RNA with A6 bulge loop

In order to understand the behavior of RNAs with large bulges In solution, molecular dynamics was performed on the RNA molecule in water with A6 bulge. The result of simulation showed that nonstacked conformation Is the main conformation in large bulges, and the backbone of large bulge is of great conformational flexibility, but bulges-induced bends are relatively rigid. The fluctuation in bulge has little influence on the bend angle of RNAs.

银鲫肌酸激酶M3-CK cDNA的克隆及其表达特征

用抑制性差减杂交结合SMARTcDNA合成和RACE PCR技术克隆到雌核发育银鲫 (Carassiusauratusgibelio)肌酸激酶M 3 CK基因的全长cDNA。银鲫M 3 CKcDNA全长 15 5 1bp ,编码 380个氨基酸 ,与普通鲤鱼(Cyprinuscarpio)M3 CK的氨基酸序列同源性高达 95 %。种系分析表明 ,银鲫M3 CK与其它脊椎动物的肌肉型肌酸激酶聚为较近的一支 ,与鲤鱼的M 3 CK聚在一起 ,与脑特异型肌酸激酶及线粒体型肌酸激酶分歧较大。虚拟Northern

Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents.

Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents.

海底管道沉降特性的数值模拟分析

本文给出了一种模拟海底管道沉降的有限元分析方法.在该方法中,海床土体采用弹塑性帽盖本构模型进行模拟;管土接触面采用‘接触对'(contact pair)算法进行处理,解决了管道嵌入海床的动边界接触问题.数值模拟显示,随着管道水下重量的增大,管道下方土体的塑性区域逐渐扩展,相应的管道沉降量也逐渐增大,并明显高于弹性模型的计算值.通过参量分析,还探讨了土体和管道特性对管道沉降量的影响.

Non-die explosive forming of spherical pressure-vessels

This paper presents a newly developed method of manufacturing spherical pressure vessels based on the technology of non-die explosive forming. Compared with the traditional method, this technology does not need any dies and pressing equipment, so that the cost of the production process can be greatly reduced, especially for vessels of less than 100 m3 capacity.

Magnetic properties of sintered sm2fe17ny magnets

Sintered magnets of Sm2Fe17Ny nitrides, with a density of 6.0-7.4 g/cm3, have been prepared by using an explosion technique. Both crystalline structure and the magnetic properties of Sm2Fe17Ny nitrides were retained in the process. The sintered magnet had a remanence B(r)=0.83 T, an intrinsic coercivity mu(0i)H(c)=0.57 T and an energy product (BH)max=88 kJ/m3. The temperature dependence of coercivity and remanence were also measured. The temperature coefficients alpha of remanence and beta of coercivity are -0.076%/degrees-C and -0.51%/degrees-C, respectively.

空气分级燃烧器稳焰器降低NO_x试验研究.

三次空气分级天然气燃烧器中稳焰器可以大幅度降低不完全燃烧热损失,但却提高了 NOx 的排放浓度,其最佳工况点为天然气管与一次风管、二次风管和三次风管的流通 面积之比为1 ∶5. 51 ∶1. 92 ∶3. 97 及天然气管与一次风管、二次风管的插深之比为 1 ∶0. 75 ∶0. 208 3 。试验表明,稳焰器的安装位置影响NOx 的排放量,烟气中NOx 最 低排放量为24. 26 mg/ m3 。

反射型体全息光栅对超短脉冲激光光束衍射的性质

在光栅记录介质色散效应的影响下，拓展Kogelnik的耦合波理论研究了反射型体全息光栅对偏振方向垂直和平行于入射面的超短脉冲激光光束衍射的性质。结果表明对于记录在LiNbO3光折变晶体中的体全息光栅，在色散效应、光栅参量和入射条件的共同影响下，光栅对偏振方向垂直于入射面的超短脉冲激光光束衍射的光谱带宽大于对偏振方向平行于入射面的超短脉冲激光光束衍射的光谱带宽，在不考虑光栅介质色散效应的影响时，它们都变大。进一步给出了衍射光的光谱带宽及光栅的衍射效率随入射脉冲的光谱带宽与光栅的光谱带宽比值的变化情况。

半导体激光直接倍频的488nm蓝光激光器

利用波导型准相位匹配周期极化反转铌酸锂（PPLN）晶体直接倍频波长为976nm的连续半导体激光二极管，在最佳晶体工作温度（28℃）下，获得了波长为488nm的连续蓝光输出，最大输出功率大于20mW。所用的晶体尺寸为8mm×1．4mm×1mm，波导截面为4．5μm×3．5μm，极化周期为5．2μm。研究了波导型周期极化反转铌酸锂晶体的倍频效率与温度的关系，与普通的周期极化反转铌酸锂相比，倍频效率与温度关系的敏感度较低。同时，由于晶体可以在室温下工作，简化了加温与温控部件，提高了整机的工作效率。在此实验的基础

Enhanced broadband near-infrared luminescence from transparent Yb 3+/Ni2+ codoped silicate glass ceramics

The near-infrared emission intensity of Ni2+ in Yb3+/Ni2+ codoped transparent MgO-Al2O3-Ga2O3-SiO2-TiO2 glass ceramics could be enhanced up to 4.4 times via energy transfer from Yb3+ to Ni2+ in nanocrystals. The best Yb2O3 concentration was about 1.00 mol%. For the Yb3+/Ni2+ codoped glass ceramic with 1.00 mol% Yb2O3, a broadband near-infrared emission centered at 1265 nm with full width at half maximum of about 300 nm and lifetime of about 220 mu s was observed. The energy transfer mechanism was also discussed. (C) 2008 Optical Society of America.

猕猴精浆纤溶酶原激活因子的来源及在精子获能中的作用

猕猴附睾、前列腺和精囊均表达tPA、uPA和PAI-1 mRNAs。加入uPA能维持精子的活力,使精子产生超激活运动,诱导顶体反应的发生,并使精子获得激活卵子的能力。这说明猕猴精浆PA除来源于睾丸外,可能主要来源于附睾及附性腺;在体外,uPA,而不是tPA,可能诱导精子获能。

Origin of rabbit (Oryctolagus cuniculus) in China: evidence from mitochondrial DNA control region sequence analysis

A fragment of mitochondrial DNA (mtDNA) control region (similar to700 bp) was sequenced in 104 individuals from 20 breeds (three Chinese domestic breeds, five recently derived breeds and 12 introduced breeds) of domestic rabbits, Oryctolagus cuniculus . Nineteen sites were polymorphic, with 18 transitions and one insertion/deletion, and eight haplotypes (A1, A2, A3, A4, A5, A6, A7 and A8) were identified. Haplotype A1 was the most common and occurred in 89 individuals. In the 25 Chinese rabbits, only haplotype A1 was observed, while four haplotypes (A1, A3, A5 and A6) were found in 26 recently derived individuals. Haplotype A2 was shared by seven individuals among three introduced strains. The other six haplotypes accounted for 0. 96-1. 92% of the animals. Combined with the published sequences of European rabbits, a reduced median-joining network was constructed. The Chinese rabbit mtDNAs were scattered into two clusters of European rabbits. These results suggest that the (so-called) Chinese rabbits were introduced from Europe. Genetic diversity in Chinese rabbits was very low.