小麦春化相关基因元件克隆分析与小麦矮化突变体的研究

一、 春化相关基因全长cDNA序列及其启动子的克隆与分析研究 通过建立小麦（Triticum aestivum. L. cv Jingdong No.1）胚芽春化cDNA文库，以春化相关基因VER2的3’端序列为探针，筛选获得全长1195 bp cDNA序列，它编码300个氨基酸。在VER2中存在植物疾病抗性反应蛋白和茉莉酸诱导凝集素两种蛋白的结构域。另外在VER2蛋白中存在核定位信号和多种磷酸酶的作用位点，VER2可能参与了多种调控途径。 以VER2基因的cDNA为探针，利用改进的池式PCR以及高密度膜杂交筛选的方法，从小麦TAC基因组文库中获得41,788 bp的基因组克隆，该序列含有11个基因，其中VER2基因位于第三个基因。VER2基因组序列含有3个内含子，4个外显子与cDNA序列100%同源。通过对转录起始点和转录终止点的分析，进一步证明从cDNA文库筛选得到的VER2基因为全长序列。 对VER2基因的上游启动子区域进行分析，发现基因上游启动子区存在三个小的重复序列，每个片段有482 bp，另有两个较大的重复序列，每个片段有2,161 bp。对上游2.8 kb启动子区（不含重复序列）的响应元件分析，其包括ABA响应元件（ABRE）、茉莉酸甲酯响应元件（Me-JARE）、胚乳特异性表达元件、参与淀粉酶合成的元件以及存在类似GA响应元件（ATAACAAAC）如ATAACATAC等等。根据VER2基因上游6 kb序列结构特点，将VER2启动子区域进行缺失突变形成10个片段，分别以GUS和GFP为报告基因构建成瞬间表达载体和植物表达载体等四类质粒。通过基因枪方法将最大片段（6 kb）驱动GFP报告基因的瞬间表达载体转入经春化处理或未春化处理的小麦幼叶中，结果发现GFP在春化处理的幼叶中表达，而在未春化处理的幼叶中不表达，说明VER2基因的启动子驱动基因转录受春化处理调控。 二、 小麦矮化突变体的研究 通过对小麦矮化突变体gaid遗传生理分析发现该突变体为半显性阻断GA信号途径，由此发现在赤霉素信号途径中，α-淀粉酶的诱导一定程度上通过某些与株高相关的基因控制。突变体gaid呈现对高浓度的脱落酸更敏感，当ABA浓度达到10-6M时，突变体的生长几乎完全受到了抑制，而野生型的生长需要ABA浓度达到10-5M时才能完全受到抑制。通过突变体gaid对乙烯等抑制型生长调节剂的响应实验研究，首次提出GA调控植物伸长生长存在两条信号途径，即GA基础水平信号途径（GA basal level signaling pathway）和GA正常水平信号途径（GA normal level signaling pathway），而乙烯以及高浓的GA合成抑制剂（如PAC）是通过第一条途径（GA基础水平信号途径）起作用。光形态建成中对植株生长的抑制作用存在独立于GA的信号途径。 突变体gaid的根系在强光照（63.5 Es-1m-2）和培养基内（低氧）的生长条件下，表现出弯曲、变短、加粗等异常性状，而随光照强度的减弱，这种根系异常生长的表型也减弱，在暗培养中则完全消失，但无论在哪种环境条件下，相对野生型对照而言，突变体的种子根短、侧根少。低浓度的ABA（10-8M）可以恢复突变体gaid根系在强光低氧条件下的正常生长发育。然而利用IAA及其极性运输的抑制剂（TIBA）、乙烯生物合成前提物（ACC）及合成抑制剂（AOA）处理突变体gaid，并没有发现突变体根系的生长发育得到恢复。 突变体gaid可能是一个新的属于小麦GA信号途径中的负调控基因（GAID）发生了突变或超表达，导致其负调控作用增强，呈现半显性的矮化突变。在与另一已知小麦GA信号途径中的负调控基因RHT的关系研究上发现，GAID可能对RHT蛋白磷酸化后的降解途径起抑制作用。通过双向电泳发现突变体gaid与野生型对照（京冬1号）在生长过程中存在差异蛋白，这将有助于对GA信号途径分子机理的深入研究。

水稻OsGSR1调控赤霉素和油菜素内酯信号互作的分子机理研究

赤霉素（gibberellins，GAs）和油菜甾醇（brassinosteroids，BRs）在细胞伸长和植株形态建成等方面发挥重要的生理作用，但它们在分子水平上的相互作用仍然未知。在本实验室前期的芯片工作中筛选到受GA诱导表达的GAST家族基因OsGSR1(GA-stimulated gene in rice) (GenBank AY604180)。该基因全长cDNA为588 bp，编码110个氨基酸，OsGSR1具有GAST家族成员的共同特点。OsGSR1基因的表达受GA3诱导，同时受PAC抑制。基因表达模式分析表明OsGSR1在水稻的根、茎、幼穗和小花等多种组织和器官中表达。前期工作已获得转基因水稻。 本论文研究表明，OsGSR1 RNAi转基因水稻表现为初生根缩短、叶片直立、节间缩短和结实率降低等与GA和BR相关的表型。OsGSR1 RNAi转基因水稻对外源GA3敏感性降低，Real-time PCR分析表明在OsGSR1 RNAi转基因水稻中OsGA20ox2和SLR1的转录水平增高，GC-MS分析显示内源GA4含量增高，这些结果说明转基因材料中GA信号削弱。因此，OsGSR1是GA信号途径的正调控因子。另一方面，实验证据表明，外源BL处理可以抑制OsGSR1基因的表达，OsGSR1 RNAi转基因水稻不但可以响应外源BL处理，并且在叶夹角实验中显现出对外源BL更加敏感的特性。在OsGSR1 RNAi转基因水稻中，BR受体基因OsBRI1与合成基因OsDWARF表达量上调。外源添加BL可以恢复OsGSR1 RNAi转基因水稻矮化表型，上述结果说明OsGSR1可能作用于BR生物合成途径。酵母双杂交筛选、体外Pull-down结果和体内BiFC实验都证实OsGSR1可以与DIM/DWF1互作。在BR生物合成途径中，DIM/DWF1催化从24-亚甲基固醇（24-methylenecholesterol）到油菜甾醇（campesterol）的转化。GC-MS测定内源BRs含量结果进一步证实，转基因水稻中DIM/DWF1催化反应产物积累量减少，说明该反应受到明显抑制。所以，OsGSR1是通过直接作用于BR合成酶来调控BR生物合成。 综上所述，OsGSR1是GA信号途径的正调控因子，并且OsGSR1通过调节SLR1的表达参与到GA信号转导途径。OsGSR1和DIM/DWF1的互作说明OsGSR1直接参与了BR的生物合成过程。因此，我们的实验证明OsGSR1介导了GA和BR这两条激素信号转导途径的相互作用，从而调节了水稻植株的生长发育。

Phylogenetic analysis of snow sheep (Ovis nivicola) and closely related taxa

Based on mitochondrial cytochrome b gene sequence analysis, the history of true sheep ( Ovis) began approximately 3.12 million years ago ( MYA). The evolution of Ovis resulted in three generally accepted genetic groups: Argaliforms, Moufloniforms, and Pac

HIV-1 C亚型密码子优化gp120基因重组腺病毒载体的构建及表达

目的 构建含有HIV-1 C亚型gp120基因重组腺病毒载体,并在293细胞中表达gp120蛋白.方法 PCR扩增,获得HIV-1 C亚型gp120片段,定向克隆入腺病毒转移载体pTrack-CMV,线性化后转化至含有腺病毒骨架载体pAd-easy-1的大肠埃希菌BJ5183,获得重组子prAd-gp120,PacⅠ酶切纯化后转染293细胞,包装成复制缺陷型重组腺病毒vAd-gp120.结果 经PCR、酶切及DNA测序,插入片段大小、方向正确,获得了具有感染力的vAd-gp120重组腺病毒;通过Western 印迹检测,重组腺病毒在293细胞中表达出分子量为120 kD的蛋白.结论 成功构建了含有HIV-1 C亚型gp120基因重组腺病毒载体,并获得该基因的表达.

Effects of polyaluminum chloride and copper sulfate on phosphorus and UV254 under different anoxic levels

Experimental sediments and water from shallow, eutrophic Dianchi Lakes were treated in a controlled laboratory microcosm using different chemicals under different anoxic levels. This study revealed that the polyaluminum chloride (PAC) was able to inhibit the phosphorus release and decrease the UV254 value at any anoxic level. When the DO concentrations were between 0.76-0.95 mg(.) L-1, the UV(254)value, total phosphorus (TP), and total dissolved phosphorus (TDP) in the water column were decreased by 71.93%, 87.12% and 64.24% respectively. The UV254, TP, and TDP were also decreased by 72.94%, 70.87% and 50.76% respectively at the levels of 4.56-5.32mg(.)L(-1) of DO concentrations. The treatment effects of TP and TDP in the water column using copper sulfate however were not as efficient as the PAC treatment. The UV254 value was increased with the addition of copper sulfate at every anoxic level tested but the chlorophyll-a (Chl-a) content was decreased rapidly and efficiently by copper sulfate more than the treatment by PAC. When the DO concentrations were 0.76-0.86mg(.)L(-1) and 4.75-5.14mg(.)L(-1), the Chl-a concentrations were decreased by 84.87% and 75.07% respectively through copper sulfate treatment. With additions of PAC and copper sulfate, the phosphorus fractions in sediments were shifted forward to the favorable shapes that have little ability of release. The TP concentrations in sediments were increased after treatment via PAC and copper sulfate. Under anoxic conditions, most of the BD-P (Fe-P) to NaOH-P (Al-P) was converted using the recommended PAC dose in BD-P rich sediment. Similar to the PAC, the copper sulfate also could flocculate the exchange phosphorus from sediment to overlying water. Overall though, the effects of copper sulfate treatment were not better than that of the PAC.

Retention of the developmental pluripotency in medaka embryonic stem cells after gene transfer and long-term drug selection for gene targeting in fish

Embryonic stem (ES) cells provide a unique tool for introducing random or targeted genetic alterations, because it is possible that the desired, but extremely rare recombinant genotypes can be screened by drug selection. ES cell-mediated transgenesis has so far been limited to the mouse. In the fish medaka (Oryzias latipes) several ES cell lines have been made available. Here we report the optimized conditions for gene transfer and drug selection in the medaka ES cell line MES1 as a prelude for gene targeting in fish. MES1 cells gave rise to a moderate to high transfection efficiency by the calcium phosphate co-precipitation (5%), commercial reagents Fugene (11%), GeneJuice (21%) and electroporation (>30%). Transient gene transfer and CAT reporter assay revealed that several enhancers/promoters and their combinations including CMV, RSV and ST (the SV40 virus early gene enhancer linked to the thymidine kinase promoter) were suitable regulatory sequences to drive transgene expression in the MES1 cells. We show that neo, hyg or pac conferred resistance to G418, hygromycin or puromycin for positive selection, while the HSV-tk generated sensitivity to ganciclovir for negative selection. The positive-negative selection procedure that is widely used for gene targeting in mouse ES cells was found to be effective also in MES1 cells. Importantly, we demonstrate that MES1 cells after gene transfer and long-term drug selection retained the developmental pluripotency, as they were able to undergo induced differentiation in vitro and to contribute to various tissues and organs during chimeric embryogenesis.

Study on the hydrolysis/precipitation behavior of Keggin Al-13 and Al-30 polymers in polyaluminum solutions

The hydrolysis/precipitation behaviors of Al3+, Al-13 and Al-30 under conditions typical for flocculation in water treatment were investigated by studying the particulates' size development, charge characteristics, chemical species and speciation transformation of coagulant hydrolysis precipitates. The optimal pH conditions for hydrolysis precipitates formation for AlCl3, PAC(A113) and PAC(A130) were 6.5-7.5, 8.5-9.5, and 7.5-9.5, respectively. The precipitates' formation rate increased with the increase in dosage, and the relative rates were AlCl3 >> PAC(A130) > PACA113. The precipitates' size increased when the dosage increased from 50 mu M to 200 mu M, but it decreased when the dosage increased to 800 AM. The Zeta potential of coagulant hydrolysis precipitates decreased with the increase in pH for the three coagulants. The isoelectric points of the freshly formed precipitates for AlCl3, PAC(A113) and PAC(A130) were 7.3, 9.6 and 9.2, respectively. The Zeta potentials of AlCl3 hydrolysis precipitates were lower than those of PAC(A113) and PAC(A130) when pH > 5.0. The Zeta potential of PAC(A130) hydrolysis precipitates was higher than that of PACA113 at the acidic side, but lower at the alkaline side. The dosage had no obvious effect on the Zeta potential of hydrolysis precipitates under fixed pH conditions. The increase in Zeta potential with the increase in dosage under uncontrolled pH conditions was due to the pH depression caused by coagulant addition. Al-Ferron research indicated that the hydrolysis precipitates of AlCl3 were composed of amorphous AI(OH)3 precipitates, but those of PACA113 and PACA130 were composed of aggregates of Al-13 and Al-30, respectively. Al3+ was the most un-stable species in coagulants, and its hydrolysis was remarkably influenced by solution pH. Al-13 and Al-30 species were very stable, and solution pH and aging had little effect on the chemical species of their hydrolysis products. The research method involving coagulant hydrolysis precipitates based on Al-Ferron reaction kinetics was studied in detail. The Al species classification based on complex reaction kinetic of hydrolysis precipitates and Ferron reagent was different from that measured in a conventional coagulant assay using the Al--Ferron method. The chemical composition of Al-a, Al-b and Al-c depended on coagulant and solution pH. The Al-b measured in the current case was different from Keggin Al-13, and the high Alb content in the AlCl3 hydrolysis precipitates could not used as testimony that most of the Al3+ Was converted to highly charged Al-13 species during AlCl3 coagulation.

重组腺病毒载体vAd2tat 的构建及其在细胞中的表达

　目的　构建含HIV21 tat 基因重组腺病毒,观察在不同细胞中外源蛋白Tat 的表达,作为DC 抗HIV 疫苗的基 础。方法　通过PCR 扩增,获得HXB2 tat 的cDNA 片段,定向克隆入腺病毒转移载体pTrack2CMV ,线性化后转化含有腺病毒骨 架pAd2easy21 的大肠杆菌BJ5183 ,获得同源重组的质粒prAd2tat , Pac Ⅰ酶切纯化后转染293 细胞,包装成具有感染力的复制缺 陷型重组腺病毒vAd2tat 。结果　经PCR、酶切及DNA 序列测定,插入片段大小、方向正确,获得具有感染力的含有HIV21 tat 基 因的重组腺病毒;通过Western blot 方法检测,重组腺病毒在293 细胞中表达出Mr 为15 000 的蛋白。结论　成功构建了含有 HIV21 tat 基因的腺病毒,并观察到该基因在细胞中的表达。

钻井废水可生化性研究及处理技术探索

钻井废水是油气井开采钻探过程中产生的废水，钻井废水成分复杂，有机物浓度高、色度高、悬浮物浓度高，水质变化大，排放点分散，不经处理排放会污染环境，破坏生态。随着石油工业的不断发展和国家环保法律法规的日益严格，钻井废水的治理也越来越受到重视。如何采用经济有效的方法处理废弃钻井液，对油气井开采业的可持续发展具有重要意义。本论文以遂宁磨153 井的钻井废水为主要研究对象，在对废水进行絮凝沉降预处理和生物法处理探索的基础上，针对钻井废水可生化性差的特点，采用水解酸化和Fenton 试剂改善钻井废水的可生化性，对反应过程进行了比较详细的考察，对可生化性改善的机理进行了探索。主要研究结论如下：1 用PFS 和PAC 配制的混合混凝剂对钻井废水COD 的去除效果比较显著，在最佳条件下COD 的去除率可达75%，且絮体沉降速度较快，出水pH 保持中性；2 水解酸化法处理钻井废水可显著改善废水的可生化性。经48 小时水解酸化处理，钻井废水的理论BOD5可提高约22 倍，表观BOD5/COD值由0.004 提高到0.034。用接触氧化反应器处理经水解酸化处理后的废水，处理效果比较稳定，COD平均去除率达35.5%；3 研究了Fenton反应中各影响因子对废水COD去除率、BOD5/COD的影响并分析其作用机制，确定了最佳条件：初始pH为4.0，H2O2/Fe2+(摩尔浓度比)为20，H2O2/COD（质量浓度比）为1，反应时间为2 个小时。此条件下，废水的COD去除率约为40%，BOD5/COD值从0.002～0.003 提高至0.15～0.2，可生化性得到很大提高。本论文的主要创新点在于：1 以成分复杂、水质变化大的气井钻井废水为研究对象，从理论BOD 和表观BOD 两方面对水解酸化过程中废水可生化性的变化进行了分析；2 对Fenton 试剂改善钻井废水可生化性的过程、主要影响因素进行了比较详细的考察。本论文的研究成果，可为生物法处理钻井废水的深入研究提供理论依据。Drilling wastewater is produced in the process of oil-gas well drilling,because of its complicated composition, high concentrate of organic compound andsuspended solid, high chroma, levity of water quality and decentralization ofdischarge point, it pollutes environment seriously if discharged without treatment.With the development of petroleum industry and the issuing of more strict laws forenvironmental protection, it has been paid more and more attention on drillingwastewater treatment. It is of great importance for the sustainable development ofoil-gas well drilling to treat drilling wastewater by economical and effective methods.In this paper, drilling wastewater of Mo No.153 well in Suining was studied asthe main object. On the basis of research on pre-treatment with flocculant andbiological treatment, and according to the character of poor biodegradability, thedrilling wastewater was treated by hydrolytic acidification and Fenton’s reagent toimprove its biodegradability. The process and mechanism of biodegradabilitychanging were investigated. The primary conclusions are:1 It is effective to treat drilling wastewater with mixing PFS and PAC asflocculant. The removal rates of COD came up to 75% under optimal conditions, thesedimentation rate of flocculation is rapid, and the pH value of treated water remainedneutral;2 The biodegradability of drilling wastewater was highly improved afterhydrolytic acidification process. The theoretic BOD5 of drilling wastewater increasedby 22 times and its detected BOD5/COD ratio increased from 0.004 to 0.034 afterhydrolytic acidification for 48 hours. The wastewater after hydrolytic acidificationwas treated by biological contact oxidation reactor. Stable treatment performance was achieved, and the average removal rates of COD came up to 35.5%;3 The effects of various affection factors on the removal efficiency of COD andBOD5/COD radio in treating drilling wastewater by Fenton’s reagent wereinvestigated and the mechanism was analyzed. The optimal conditions were: initialpH of solution was 4.0, the molar ratio of H2O2 and Fe2+ was 20, the concentrationratio of H2O2 and COD was 1 and the reaction time was 120 min. Under the aboveconditions, the removal efficiency was about 40% and the ratio of BOD5 and CODincreased from 0.002 ¡« 0.003 to 0.15 ¡« 0.2. The biodegradability of drillingwastewater was greatly improved.The innovations of this thesis are:1 The drilling wastewater was taken as the research object which hascomplicated composition and variational water quality, and the changes ofbiodegradability were analyzed from theoretic BOD and detected BOD aspects duringhydrolytic acidification process;2 The biodegradability changing process and primary affection factors of drillingwastewater treating by Fenton’s reagent were investigated.The results of this study could provide theoretic foundation for further researchon biological treatment of drilling wastewater.

离子源分布式控制系统设计

将NI公司的可编程自动化控制器(PAC)CompactFieldPoint应用于离子源系统控制中,可充分发其易于使用、可扩展性强、可重用度高以及具有实时的运载软件的平台,结合稳定的I/O模块实现ECR离子源的分布式网络系统控制。使用PID控制算法控制定标参量;并通过远程面板技术,用户通过本地(Client端)计算机打开并操作位于远程(WebServer端)上的VI前面板,实现了测试数据的远程共享和用户对系统的远程操控。

基于OPC技术离子源控制系统研究

采用ICPDAS公司的可编程自动化控制器(PAC)Wincon8000结合稳定的I/O模块,实现基于OPC技术规范的离子源网络系统控制。研究了OPC技术在现场设备间的开放式数据交换和异构网间的数据共享,并实现大量数据源通信的标准机制。

Vortices in (Abelian) Chern-Simons gauge theory

The vortex solutions of various classical planar field theories with (Abelian) Chern-Simons term are reviewed. Relativistic vortices, put forward by Paul and Khare, arise when the Abelian Higgs model is augmented with the Chern-Simons term. Adding a suitable sixth-order potential and turning off the Maxwell term provides us with pure Chern-Simons theory, with both topological and non-topological self-dual vortices, as found by Hong-Kim-Pac, and by Jackiw-Lee-Weinberg. The non-relativistic limit of the latter leads to non-topological Jackiw-Pi vortices with a pure fourth-order potential. Explicit solutions are found by solving the Liouville equation. The scalar matter field can be replaced by spinors, leading to fermionic vortices. Alternatively, topological vortices in external field are constructed in the phenomenological model proposed by Zhang-Hansson-Kivelson. Non-relativistic Maxwell-Chern-Simons vortices are also studied. The Schrodinger symmetry of Jackiw-Pi vortices, as well as the construction of some time-dependent vortices, can be explained by the conformal properties of non-relativistic space-time, derived in a Kaluza-Klein-type framework. (c) 2009 Elsevier B.V. All rights reserved.

改性粘土治理有害赤潮对生态环境的影响初探

本文以室内实验为基础，研究了改性粘土治理有害赤潮的方法对生态环境的影响。研究结果表明：有机和无机改性粘土对溶解氧（DO）、化学耗氧量（COD）、pH等主要水质因子均有改善作用；对营养盐，尤其是磷酸盐有一定的吸附作用，在外加磷酸盐为0-0.3μmol/ml范围内，吸附量随水体中磷酸盐浓度的增加而增大，有机改性粘土对海水中磷酸盐的吸附能力为：有机改性粘土Ⅰ>有机改性粘土Ⅱ>有机改性粘土Ⅲ。通过有机改性粘土对磷酸盐的吸附－再释放情况，进一步探讨了其磷酸盐的释放对赤潮异弯藻（Heterosigma akashiwo）、东海原甲藻（Prorocentrum donghaiense）等赤潮生物生长的影响。实验结果显示，经过有机改性的粘土有利于提高其对磷酸盐的吸附能力，降低对磷酸盐的解吸率，利用有机改性粘土治理赤潮可以缓解海水富营养化程度，即使被吸附的部分磷酸盐能缓慢释放，但仍不足以维持赤潮生物的正常生长。无机改性粘土对磷酸盐和硝酸盐与有机改性粘土有相似的吸附性能。其添加剂PAC的重金属含量符合沉积物排放要求，不会对环境造成压力。 其次，以太平洋牡蛎(Crassostrea gigas)幼贝为对象，研究了有机改性粘土Ⅱ和无机改性粘土在对海洋底栖生物的影响。急性毒性试验中二者对牡蛎幼贝的半致死浓度（LC50）分别为4.62 g/L和2.67 g/L，均远远大于改性粘土去除赤潮藻的浓度。在能够有效去除赤潮微藻的粘土浓度条件下，经慢性毒性试验发现改性粘土对牡蛎幼贝成活率、生长和摄食略有降低，但无明显影响，在牡蛎幼贝鳃组织和消化道组织的超微结构中未发现机械损伤。 最后，研究了改性粘土在对去除有毒赤潮藻过程中对底栖生物的影响。以能产生麻痹性贝毒（PSP）的塔玛亚历山大藻（A. tamarense，ATHK）为例，研究了PSP在水体中和粘土沉积层中的分布，发现改性粘土去除塔玛亚历山大藻能有效降低水体中藻细胞浓度及其所携带的PSP毒素。由于藻细胞破裂, 虽然沉积层中藻细胞体内部分PSP毒素溢出，但通过塔玛亚历山大藻细胞不同组分对牡蛎的影响发现，细胞内容物和细胞碎片对牡蛎没有明显毒副作用，由此推断改性粘土对藻细胞絮凝沉降后,能降低对生物的不良影响。此外，室内实验模拟了去除赤潮生物塔玛亚历山大藻（A. tamarense，ATHK）、东海原甲藻 (P. donghaiense )、赤潮异弯藻 (H. akashiwo)过程中，比较改性粘土和赤潮微藻对生物体的影响发现改性粘土在去除赤潮藻过程中能提高牡蛎的存活率，进一步证明改性粘土在赤潮生物防控中，是一种有效的应急措施。

Determination of five pharmacologically active compounds extracted from Rhodiola for natural product drug discovery with HPLC-APCI-MS

A rapid and sensitive liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS) assay for the determination of five pharmacologically active compounds (PAC) extracted from the traditional Chinese medicine, Rhodiola , namely salidroside, tyrosol, rhodionin, gallic acid, and ethyl gallate has been developed. In this method, PAC could be baseline separated and detected with DAD at 275 nm. The validation of the method, including sensitivity, linearity, repeatability, and recovery, was examined. The linear calibration curves were acquired with correlation coefficient >0.999 and the limits of detection LOD (at a signal-to-noise ratio=3:1) were between 0.058 and 1.500 mu mol/L. It was found, that the amounts of PAC varied with different species of Rhodiola . The established method is rapid and reproducible for the separation of five natural pharmacologically active compounds from extracts of Rhodiola with satisfactory results.

一类新型的废水处理药剂—PSAFC的研制及对印染废水的处理

利用AI^3+、Fe^3+的共聚合特性和硅酸的盐效应机制，选择廉价而广泛的原料：工业用的AICI3、废铁屑或硫铁矿提取硫酸后的废渣、工业用水玻璃以及工业盐酸，在常温常压下聚合出的PSAFC废水处理药剂，对贵阳棉纺厂的几种印染废水在实验室条件下进行处理，处理效果比目前市场上普遍使用的碱式氯化铝（PAC）的效果要好。